Minor Changes in Erythrocyte Osmotic Fragility in Trace Amine-Associated Receptor 5 (TAAR5) Knockout Mice

Zhukov, Ilya S.; Kubarskaya, L. G.; Karpova, I. V.; Vaganova, Anastasia N.; Karpenko, Marina N.; Gainetdinov, Raul R.

doi:10.3390/ijms22147307

Cited by 6 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As is known to all, the biconcave shape and corresponding deformability of the RBCs is an essential feature of its biological function [ 42 ]. This feature of RBCs may be affected during the incubation with liposomes.…”

Section: Resultsmentioning

confidence: 99%

Hybrid liposome–erythrocyte drug delivery system for tumor therapy with enhanced targeting and blood circulation

Zhu

Zhong

et al. 2023

Regenerative Biomaterials

View full text Add to dashboard Cite

Liposome, a widely used drug delivery system (DDS), still shows several disadvantages such as dominant clearance by liver and poor target organ deposition. To overcome the drawbacks of liposomes, we developed a novel red blood cell (RBC)-liposome combined DDS to modulate the tumor accumulation and extend the blood circulation life of the existing liposomal DDS. Here, RBCs, an ideal natural carrier DDS, were utilized to carry liposomes and avoid them undergo the fast clearance in the blood. In this study, liposomes could either absorbed onto RBCs’ surface or fuse with RBCs’ membrane by merely altering the interaction time at 37 °C, while the interaction between liposome and RBCs would not affect RBCs’ characteristics. In the in-vivo anti-tumor therapeutic efficacy study, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes attached onto RBCs’ surfaces exhibited lung targeting effect (via RBC-hitchhiking approach) and reduced clearance in the liver, while DPPC liposomes fused with RBCs had prolong blood circulation up to 48 h and no enrichment in any organ. Furthermore, 20 mol% of DPPC liposomes were replaced with pH-sensitive phospholipid 1,2-Dioleoyl-Sn-glycero-3-phosphoethanolamine (DOPE) as it could respond to the low pH tumor microenvironment and then accumulate in the tumor. The DOPE attached/fusion RBCs showed partial enrichment in lung and about 5-8% tumor accumulation, which were significantly higher than (about 0.7%) of the conventional liposomal DDS. Thus, RBC-liposome composite DDS is able to improve the liposomal tumor accumulation and blood circulation, and shows the clinical application promises of using autologous RBCs for antitumor therapy.

show abstract

Section: Resultsmentioning

confidence: 99%

Hybrid liposome–erythrocyte drug delivery system for tumor therapy with enhanced targeting and blood circulation

Zhu

Zhong

et al. 2023

Regenerative Biomaterials

View full text Add to dashboard Cite

show abstract

“…The erythrocytes (hematocrit 0.4%) incubated with 0-50-100-200-400-800 mg l −1 D-PAA/ZnONPs(SO 4 2-) nanocomplex for 24 h were centrifuged (500 g) for 5 min and the pellets were resuspended in 1 ml 0.2% sodium chloride solution for 10 min at 37 °C [52]. Following the incubation, the samples were centrifuged (500 g) for 5 min and the obtained supernatant was analyzed spectrophotometrically (ULAB 102UV, China) at 541 nm.…”

Section: Osmotic Fragility Assaymentioning

confidence: 99%

Hemocompatibility of dextran-graft-polyacrylamide/zinc oxide nanosystems: hemolysis or eryptosis?

Onishchenko,

Prokopiuk,

Chumachenko

et al. 2023

Nanotechnology

View full text Add to dashboard Cite

Aim. In this study, blood compatibility of ZnO nanoparticles-nanocomplex (D-PAA/ZnONPs(SO42-)) synthesized in situ into dextran-graft-polyacrylamide (D-PAA) using zinc oxide nanoparticles produced from zinc sulphate (D-PAA/ZnONPs(SO42-)) as a precursor was tested using hemolysis, osmotic fragility and eryptosis assays.Materials and methods. Dose-dependent ability to induce eryptosis was assessed following 24 h incubation at concentrations of 0-800 mg / L analyzing hallmarks of eryptosis (cell shrinkage and phosphatidylserine externalization), as well as reactive oxygen species (ROS) generation. Hemolysis was detected spectrophotometrically based on hemoglobin release following exposure to the D-PAA/ZnONPs(SO42-) nanocomplex. Osmotic fragility test (OFT) involved detection of hemolysis of red blood cells exposed to 0.2% saline solution following incubation with the D-PAA/ZnONPs(SO42-) nanocomplex. Additional incubation of the nanocomplex in the presence or absence of either ascorbic acid or EGTA was used to reveal the implication of oxidative stress- or Ca2+-mediated mechanisms in D-PAA/ZnONPs(SO42-) nanocomplex-induced erythrotoxicity.Results. Hemocompatibility assessment of the D-PAA/ZnONPs(SO42-) nanocomplex revealed that it induced hemolysis and reduced resistance of erythrocytes to osmotic stress at concentrations of above 400 and 200 mg / L, respectively. Oxidative stress- or Ca2+-mediated mechanisms were not involved in D-PAA/ZnONPs(SO42-) nanocomplex-induced hemolysis. Strikingly, the D-PAA/ZnONPs(SO42-) nanocomplex did not promote cell membrane scrambling, cell shrinkage and oxidative stress in red blood cells following the direct exposure for 24 h. Thus, the D-PAA/ZnONPs(SO42-) nanocomplex did not induce eryptosis in vitro. Eryptosis is generally considered to occur earlier than hemolysis in response to stress in order to prevent hemolytic cell death. Counterintuitively, our data suggest that hemolysis can be triggered by nanomaterials prior to eryptosis indicating that eryptosis and hemolysis assays should be used in combination for testing blood compatibility of nanomaterials. Conclusions. The D-PAA/ZnONPs(SO42-) nanocomplex has a good hemocompatibility profile at low concentrations. Hemocompatibility testing in nanotoxicology should include both eryptosis and hemolysis assays. 

show abstract

“…The latest hematological and biochemical studies demonstrate the importance of the TAAR receptor family in the periphery. It was found that deletion of the TAAR5 receptor gene leads to erythrocyte fragility changes [ 53 ]. Furthermore, TAAR9 knockout rats demonstrated decreased low-density lipoprotein cholesterol levels in comparison to control rats [ 54 ].…”

Section: Introductionmentioning

confidence: 99%

Protein Metabolism Changes and Alterations in Behavior of Trace Amine-Associated Receptor 1 Knockout Mice Fed a High-Fructose Diet

Apryatin

Zhukov

Zolotoverkhaya³

et al. 2023

Neurology International

Self Cite

View full text Add to dashboard Cite

Trace amines and their receptors are a family of G protein-coupled receptors widely distributed in the central nervous system and periphery. The trace amine-associated receptor 1 (TAAR1) plays a significant role as a therapeutic target for schizophrenia, depression, diabetes, and obesity. In this study, TAAR1 knockout mice and WT groups were tested in conditions of a high-fructose diet. The consumption of a high-fructose diet may be due to the influence on the metabolism processes by dopamine in the brain, neuromotor function, and level of anxiety of TAAR1 knockout mice. During a comparative analysis of behavioral, biochemical, and morphological parameters, significant differences were found between liver and biochemical parameters, the regulation of protein metabolism (AST/ALT ratio, creatine kinase activity, urea), and alterations in behavior. An elevated plus maze analysis showed the influence of fructose and genetic factors on the level of anxiety. A new marker of the grooming microstructure (depression ratio) was tested, which showed high efficiency as a marker of depression-like behavioral changes and a possible association with dopamine-dependent regulation of protein metabolism. These results confirm a possible association of the TAAR1 gene knockout with an increase in catabolic reaction levels by AST/ALT-dependent and possible dopamine-mediated protein metabolism regulation and depression-like behavior.

show abstract

Minor Changes in Erythrocyte Osmotic Fragility in Trace Amine-Associated Receptor 5 (TAAR5) Knockout Mice

Cited by 6 publications

References 30 publications

Hybrid liposome–erythrocyte drug delivery system for tumor therapy with enhanced targeting and blood circulation

Hybrid liposome–erythrocyte drug delivery system for tumor therapy with enhanced targeting and blood circulation

Hemocompatibility of dextran-graft-polyacrylamide/zinc oxide nanosystems: hemolysis or eryptosis?

Protein Metabolism Changes and Alterations in Behavior of Trace Amine-Associated Receptor 1 Knockout Mice Fed a High-Fructose Diet

Contact Info

Product

Resources

About