Minireview: Biophysical Mechanisms of Cell Membrane Sonopermeabilization. Knowns and Unknowns

Escoffre, Jean‐Michel; Bouakaz, Ayache

doi:10.1021/acs.langmuir.8b03538

Cited by 21 publications

(21 citation statements)

References 159 publications

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“…In Vivo Endothelial Cell Sonoporation without Tight Junction Disruption or Transcellular Transport. There are many mechanisms by which FUS and MBs stimulate cellular uptake of therapeutic agents (30,86). The oscillation of MBs close to the plasma membrane of cells has been shown to push and pull on the membrane to cause membrane deformation and pore formation (87).…”

Section: Sonoselective Transfection Of Endothelium Without Use Of Endmentioning

confidence: 99%

“…Gas-filled microbubbles (MBs) can be introduced to the circulation intravenously. These MBs expand and contract in response to the acoustic pressure waves [which, at certain frequencies, can pass through bone without excessive attenuation (25)], pushing and pulling on endothelial cells to disrupt tight junctions, enhance transcellular transport (26)(27)(28)(29), and induce transport of different molecules across the BBB (30)(31)(32)(33)(34)(35)(36). This method of using FUS in conjunction with MBs to transiently open the BBB has been used to deliver a wide range of therapeutic agents, including antibodies (37)(38)(39), proteins (40,41), nanoparticles (21,23,42), and even stem cells (43,44), to specific sites within the brain.…”

mentioning

confidence: 99%

“…The low-pressure FUS regimen results in enhanced gene delivery to endothelial cells, with none of the inflammatory or immune pathway up-regulation observed at higher FUS pressures. of central nervous system tumors (61,62), stimulation of neurogenic pathways that could permit regenerative therapies (58,63,64), and improving uptake of therapeutics from the bloodstream by increasing endocytosis and reducing small-molecule efflux (30,65). However, disruption of the BBB may not be desirable in all cases where gene therapy has potential benefits.…”

mentioning

confidence: 99%

“…These studies have demonstrated the formation of membrane pores on endothelial cells following MB oscillation-induced shear stress as well as the initiation of intercellular gaps between adjacent cells and induction of endocytosis, all of which could facilitate the delivery of therapeutic agents (36,(71)(72)(73)(74)(75)(76)(77)(78). The effects of acoustic sonoporation have been investigated in vivo as well (30,31,79), but to date no studies have utilized FUS to achieve targeted sonoporation of endothelial cells in vivo without disruption of tight junctions and/ or enhancement of transcellular transport that would allow for therapeutic delivery beyond the vasculature.…”

mentioning

confidence: 99%

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Sonoselective transfection of cerebral vasculature without blood–brain barrier disruption

Gorick

Mathew

Garrison

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Treatment of many pathologies of the brain could be improved markedly by the development of noninvasive therapeutic approaches that elicit robust, endothelial cell-selective gene expression in specific brain regions that are targeted under MR image guidance. While focused ultrasound (FUS) in conjunction with gasfilled microbubbles (MBs) has emerged as a noninvasive modality for MR image-guided gene delivery to the brain, it has been used exclusively to transiently disrupt the blood-brain barrier (BBB), which may induce a sterile inflammation response. Here, we introduce an MR image-guided FUS method that elicits endothelialselective transfection of the cerebral vasculature (i.e., "sonoselective" transfection), without opening the BBB. We first determined that activating circulating, cationic plasmid-bearing MBs with pulsed low-pressure (0.1 MPa) 1.1-MHz FUS facilitates sonoselective gene delivery to the endothelium without MRI-detectable disruption of the BBB. The degree of endothelial selectivity varied inversely with the FUS pressure, with higher pressures (i.e., 0.3-MPa and 0.4-MPa FUS) consistently inducing BBB opening and extravascular transfection. Bulk RNA sequencing analyses revealed that the sonoselective low-pressure regimen does not up-regulate inflammatory or immune responses. Single-cell RNA sequencing indicated that the transcriptome of sonoselectively transfected brain endothelium was unaffected by the treatment. The approach developed here permits targeted gene delivery to blood vessels and could be used to promote angiogenesis, release endothelial cellsecreted factors to stimulate nerve regrowth, or recruit neural stem cells.focused ultrasound | microbubbles | endothelium | gene delivery

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Section: Sonoselective Transfection Of Endothelium Without Use Of Endmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Sonoselective transfection of cerebral vasculature without blood–brain barrier disruption

Gorick

Mathew

Garrison

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…When UCAs and the targeted drug are intravenously coinjected and both exposed to ultrasound, it provides unprecedented possibilities for a selective therapeutic action known as sonoporation (Geers et al, 2012). Sonoporation denotes a process in which ultrasonically-activated microbubbles (USMB) pulsations induce a transient permeabilization of nearby endothelial barriers (e.g., blood-tumor barrier, blood-brain barrier) (Escoffre and Bouakaz, 2018). This increases vascular permeability and so extravasation of drugs from blood into surrounding tissues (Carpentier et al, 2016;Dimcevski et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Amikacin Diffusion With Ultrasound and Microbubbles in a Mechanically Ventilated Condensed Lung Rabbit Model

et al. 2020

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The poor diffusion of intravenous antibiotics in lung tissue makes nosocomial pneumonia challenging to treat, notably in critical patients under mechanical ventilation. The combination of ultrasound and microbubbles (USMB) is an emerging method for noninvasive and targeted enhancement of uptake of various drugs in several organs. This study aims to evaluate if USMB may increase amikacin concentration in condensed lung tissues in a mechanically ventilated rabbit model. When applied 60 or 160 min after the beginning of an intravenous amikacin infusion, USMB increased amikacin concentration in the condensed lung tissue by 1.33 (p = 0.025) or 1.56-fold (p = 0.028) respectively. When applied 70 min after the beginning of an intravenous amikacin infusion, USMB increased amikacin concentration in the muscle tissue by 2.52 (p = 0.025). In conclusion, this study demonstrates that USMB is a promising method for the targeted delivery of amikacin in mechanically ventilated condensed lung, thus opening new therapeutic fields against lung infections.

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Sonoporation: Past, Present, and Future

Rich

Tian

Huang

2021

Adv Materials Technologies

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only contribute to fundamental studies in areas of biology, bioengineering, and medicine, but also benefit several life-changing technologies, such as cell-based therapies, protein therapeutics, vaccines, as well as the reprogramming of cells. [22] Among the current delivery approaches, the viral vector-based approach is often considered the gold standard for the delivery of nucleic acids. [22] However, due to notable weaknesses such as biohazards, inconsistent results, and labor-intensive manufacturing, [22][23][24] physical permeabilization approaches are being pursued, [1][2][3][4][5][6][7][8][9][10][11][12]22] including electroporation, [25,26] hydroporation, [27][28][29][30][31][32] mechanoporation, [25,[32][33][34][35][36][37][38][39][40] and sonoporation. [41,42] These approaches are noted as advanced due to their high throughputs, cost-effectiveness, and universal nature to deliver a variety of cargos regardless of cell type. [3] Of the physical permeabilization approaches, sonoporation wields great potential and has been demonstrated as an efficacious technology for delivering a variety of functional cargos, such as biomolecular drugs, proteins, and plasmids, to different types of cells, such as cancer, immune, and stem cells. [43][44][45][46][47][48] Sonoporation was discovered in the 1980s, a golden age of intracellular delivery, in which quite a few physical permeabilization methods were invented. [49][50][51][52][53][54][55][56][57][58] Sonoporation enhances the intracellular delivery of cargos into cells by employing acoustic waves to disrupt their membranes. [22,[59][60][61][62] Traditional sonoporation approaches typically rely on ultrasound-induced bubble dynamic behaviors, such as inertial cavitation, noninertial (stable) cavitation, and bubble translation. [49][50][51][52] However, the bubble-based sonoporation approaches usually require special contrast agents, [63][64][65][66][67][68] which adds a new dimension of complexity and cost to the use of sonoporation. Moreover, bubble-based approaches have a high chance of inducing irreversible cell damage, lowering the cell viability, as well as reducing the effectiveness of delivered cargos. [69][70][71][72][73] Therefore, several novel non-bubble-based sonoporation approaches, such as those based on acoustic radiation force and acoustic streaming-induced shear force, are being developed to overcome the limitations of bubble-based sonoporation approaches. Although there are several review papers on the mechanisms and applications of bubble-based sonoporation, [61,62,[74][75][76][77][78][79][80][81][82][83][84][85][86][87][88] they have neglected some of the newly developed mechanisms. Therefore, this review article covers recent innovations in bubble-based and especially in non-bubble-based sonoporation. The following sections review the sonoporation mechanisms, A surge of research in intracellular delivery technologies is underway with the increased innovations in cell-based therapies and cell reprogramming. Particularly, physical cell membrane p...

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Minireview: Biophysical Mechanisms of Cell Membrane Sonopermeabilization. Knowns and Unknowns

Cited by 21 publications

References 159 publications

Sonoselective transfection of cerebral vasculature without blood–brain barrier disruption

Sonoselective transfection of cerebral vasculature without blood–brain barrier disruption

Enhanced Amikacin Diffusion With Ultrasound and Microbubbles in a Mechanically Ventilated Condensed Lung Rabbit Model

Sonoporation: Past, Present, and Future

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