“…After optimizations of nitrogen and minerals content in the media, as well as some operational parameters (pH 6.5, 30°C, and 72 h), this strain reached 34.20 U/mL cellulase activity using carboxymethyl cellulose (CMC) as substrate and 25.49 U/mL using Napier grass biomass waste powdered. After 5 days of saccharification, 72.51% of cellulose content in Napier biomass was converted to glucose 91 . C. tropicalis W2 isolated from sites with a high level of plant cells decomposition was also a cellulase producer able to grow in several substrates such as CMC, filter paper, rice straw, and wheat bagasse 92 .…”
Section: Biotechnological Applications Of C Tropicalismentioning
confidence: 99%
“…After 5 days of saccharification, 72.51% of cellulose content in Napier biomass was converted to glucose. 91 C. tropicalis W2 isolated from sites with a high level of plant cells decomposition was also a cellulase producer able to grow in several substrates such as CMC, filter paper, rice straw, and wheat bagasse. 92 This strain produced cellulase with an activity of 75 U/mg after 120 h fermentation of saw dust hydrolysate 1% supplemented with mineral salts at pH 7.0 and 37 • C. The yeast produced both intra and extracellular cellulases, with the extracellular form being predominant.…”
Candida tropicalis is a nonconventional yeast with medical and industrial significance, belonging to the CTG clade. Recent advancements in whole‐genome sequencing and genetic analysis revealed its close relation to other unconventional yeasts of biotechnological importance. C. tropicalis is known for its immense potential in synthesizing various valuable biomolecules such as ethanol, xylitol, biosurfactants, lipids, enzymes, α,ω‐dicarboxylic acids, single‐cell proteins, and more, making it an attractive target for biotechnological applications. This review provides an update on C. tropicalis biological characteristics and its efficiency in producing a diverse range of biomolecules with industrial significance from various feedstocks. The information presented in this review contributes to a better understanding of C. tropicalis and highlights its potential for biotechnological applications and market viability.
“…After optimizations of nitrogen and minerals content in the media, as well as some operational parameters (pH 6.5, 30°C, and 72 h), this strain reached 34.20 U/mL cellulase activity using carboxymethyl cellulose (CMC) as substrate and 25.49 U/mL using Napier grass biomass waste powdered. After 5 days of saccharification, 72.51% of cellulose content in Napier biomass was converted to glucose 91 . C. tropicalis W2 isolated from sites with a high level of plant cells decomposition was also a cellulase producer able to grow in several substrates such as CMC, filter paper, rice straw, and wheat bagasse 92 .…”
Section: Biotechnological Applications Of C Tropicalismentioning
confidence: 99%
“…After 5 days of saccharification, 72.51% of cellulose content in Napier biomass was converted to glucose. 91 C. tropicalis W2 isolated from sites with a high level of plant cells decomposition was also a cellulase producer able to grow in several substrates such as CMC, filter paper, rice straw, and wheat bagasse. 92 This strain produced cellulase with an activity of 75 U/mg after 120 h fermentation of saw dust hydrolysate 1% supplemented with mineral salts at pH 7.0 and 37 • C. The yeast produced both intra and extracellular cellulases, with the extracellular form being predominant.…”
Candida tropicalis is a nonconventional yeast with medical and industrial significance, belonging to the CTG clade. Recent advancements in whole‐genome sequencing and genetic analysis revealed its close relation to other unconventional yeasts of biotechnological importance. C. tropicalis is known for its immense potential in synthesizing various valuable biomolecules such as ethanol, xylitol, biosurfactants, lipids, enzymes, α,ω‐dicarboxylic acids, single‐cell proteins, and more, making it an attractive target for biotechnological applications. This review provides an update on C. tropicalis biological characteristics and its efficiency in producing a diverse range of biomolecules with industrial significance from various feedstocks. The information presented in this review contributes to a better understanding of C. tropicalis and highlights its potential for biotechnological applications and market viability.
“…Cellulase activity in microbes can be improved by nutritional and process parameters during fermentation (Rasul et al, 2015). Response surface methodology is an efficient technique utilized for optimization of fermentation processes (Valliammai et al, 2020). Mathematical models are proven tools in the optimisation of process parameters during fermentation (Imamoglu and Sukan, 2013).…”
Section: O N L I N E C O P ÿmentioning
confidence: 99%
“…Y = +23.87+ +1.26 A + 0.8132 B + 0.0277 C -0.1295 D -0.0195E -0.0135F +0.3186 AB -0.0902AC +0.0392AD - water. The decrease in enzymatic activity with increasing incubation time might be due to depletion of nutrients and production of other inhibitors during the fermentation medium (Ikram-ul-Haq et al, 2005 (Valliammai et al, 2020). RSM provided the individual and interactive effects of components in the medium during fermentation.…”
mentioning
confidence: 99%
“…Yeasts like Pichiastipitis was utilized in the saccharification study of sugarcane bagasse (Lau et al, 2010). The saccharification potential of Candida tropicalis isolate YES3 in Napier grass rd biomass was maximum on 3 day (31.93%) (Valliammai et al, 2020).…”
Aim: This study aimed to search for novel cellulolytic isolates with high cellulase titre for the production of fuels and chemicals. Methodology: The yeast isolate YES5 isolated from the forest soil was screened for cellulase production. The cellulase activity of YES5 was optimized via RSM. The saccharification potential of YES5 using Napier biomass as substrate was evalauted. Results: The maximum cellulase activity obtained after optimizing pH, temperature, and incubation period was 35.70 U. A reliable statistical model was developed for maximizing the cellulase activity in YES5 Trichosporon asahii. The cellulase activity was 23.87U, when carbon source in CMC medium was replaced by Napier biomass. The maximum saccharification potential of 33.15% was observed on 3rd day. Interpretation: The study of optimizing the media composition of Trichosporon asahii cellulase using Napier biomass, a natural source of carbon for maximizing the cellulase production via RSM, is first of its kind.
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