Aim: This study aimed to search for novel cellulolytic isolates with high cellulase titre for the production of fuels and chemicals. Methodology: The yeast isolate YES5 isolated from the forest soil was screened for cellulase production. The cellulase activity of YES5 was optimized via RSM. The saccharification potential of YES5 using Napier biomass as substrate was evalauted. Results: The maximum cellulase activity obtained after optimizing pH, temperature, and incubation period was 35.70 U. A reliable statistical model was developed for maximizing the cellulase activity in YES5 Trichosporon asahii. The cellulase activity was 23.87U, when carbon source in CMC medium was replaced by Napier biomass. The maximum saccharification potential of 33.15% was observed on 3rd day. Interpretation: The study of optimizing the media composition of Trichosporon asahii cellulase using Napier biomass, a natural source of carbon for maximizing the cellulase production via RSM, is first of its kind.
Termite soils provide a very distinct ecological environment which harbors and promotes very specialized cellulolytic and hemicellulolytic microorganisms. This specialized flora enables the termites to feed on hardly degradable polymers such as lignocellulose. The cellulase enzymes synthesized by the microorganisms have a crucial role in recycling C and nutrients and maintaining soil fertility in nature. Hence in the present study, termite mound soils were collected from different locations of Tamil Nadu and their lignocellulosic enzyme activity viz., Cellulase, Saccharase and βglucosidase were assessed to know fertility status of the particular habitats.
The biological treatment of lignocellulosic biomass through enzymatic hydrolysis serves as an ecofriendly and cost efficient method. As these biomass is the most abundant renewable resource which lacks proper utilization and disposal. This study was conducted to isolate and screen the presence of cellulosic enzymes from the microbes of natural ecosystem under laboratory condition. The microbes were isolated from elephant dung, forest soil, termites (gut) collected from Anaikatty forest range of Coimbatore district. Microbes were screened qualitatively and quantitatively for the presence of endoglucanase, exoglucanase, β-glucosidase enzymes. Among the total microbes recovered, one bacteria (BT4), one action bacteria (AS3)and two fungal isolates(FE5,FE6)showed maximum cellulolytic efficiency during qualitative screening, those were further subjected for quantitative estimation of endoglucanase, exoglucanase, β-glucosidase activity.
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