Minimum Contact Time and Concentration of Sodium Hypochlorite Required to Eliminate Enterococcus faecalis

Biofilm Dissolution and Cleaning Ability of Different Irrigant Solutions on Intraorally Infected Dentin

“…This restriction could explain the results of previous studies that required 40-60 minutes of 5.25% NaOCl to get a negative culture in the dentin block model (31,32). In addition, the present work was restricted to 30-to 50-mm biofilm thickness; a higher biomass or anatomical irregularities might increase the time necessary to dissolve bacterial biofilms in vivo.…”

Evaluation of Microbial Reduction in Root Canals Instrumented with Reciprocating and Rotary Systems

“…Whereas Radcliffe et al (23) verified that the same concentration of irrigant required 10 minutes to promote negative cultures of suspensions of E. faecalis. However, Retamozo et al (24) observed that 1.3% NaOCl was not able to disinfect dentin cylinders contaminated with E. faecalis even after 40 minutes of irrigation. On the other hand, Câmara et al (17) demonstrated that even after 4.2 ± 1.5 minutes of preparation time, 1% NaOCl was effective in disinfecting root canals infected with E. faecalis, P. aeruginosa, S. aureus e C. albicans.…”

Comparison of the Effect of Various Irrigants on Apically Extruded Debris after Root Canal Preparation

“…It has been reported that 0.5% NaOCl has the same antibacterial activity compared with 5.25%, but its tissue-dissolving ability is less (9,26,32). In contrast, one study has reported that only 5.25% NaOCl was able to render root canals free of Enterococcus faecalis when compared with 1.3% and 2.5% of the same solution (33). Surveys of dentists and endodontists in different countries have shown that in the United States the most popular root canal irrigant was 6% NaOCl for teeth with closed apical foramina (30,31), whereas in Lithuania 2%-3% NaOCl was the most popular irrigant (34).…”