Minimizing &lt;em&gt;Pseudomonas aeruginosa&lt;/em&gt; adhesion to titanium surfaces by a plasma nitriding process

Aires, Michelle de Medeiros; Treter, Janine; Filho, A. Nunes; Nascimento, Igor Oliveira; Macêdo, Alexandre José; Júnior, Clodomiro Alves

doi:10.3934/biophy.2017.1.19

Cited by 5 publications

(9 citation statements)

References 25 publications

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“…Plasma nitriding promoted an increase in the roughness parameters, as well as in surface hydrophilicity (reduced the wetting contact angle), capable of influencing pre‐osteoblast responses. Therefore, the plasma nitriding process altered the roughness and hydrophilicity properties of titanium, thereby generating a surface with potential for osseointegration …”

Section: Discussionmentioning

confidence: 99%

“…CP‐Ti (grade II) discs with pre‐established dimensions (diameter: 9 mm; thickness: 3 mm) were produced, treated, and characterized in previous projects carried out by our group at the Plasma Processing Laboratory (LabPlasma) at the Federal University of Rio Grande do Norte, Brazil . Two surfaces were evaluated: one surface only polished (PS) and one surface polished and later submitted to plasma treatment to obtain a nitrided surface (NS).…”

Section: Methodsmentioning

confidence: 99%

“…All disks were previously sanded with a silicon carbide sandpaper 80 to 2000 MESH) and the polishing was performed using a solution composed of silica colloidal and hydrogen peroxide for 30 minutes. For NS preparation, previously polished disks were conditioned in a vacuum plasma reactor to receive the plasma treatment under the following conditions: nitrogen atmosphere at 8 sccm of hydrogen and 2 sccm of nitrogen (80% H 2 + 20% N 2 ), plasma pressure of 2.2 mbar, and constant temperature of 500°C for 1 hour …”

Section: Methodsmentioning

confidence: 99%

“…Surfaces were previously developed and characterized by Aires et al The surface wettability ( θ = 70.20°, PS; θ = 45.00°, NS) was determined by the sessile drop method by mean water contact angle. Surface nanotopography and roughness were observed using a Shimadzu SPM 9600 atomic force microscope (AFM Shimadzu Corporation, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…As the nitrided titanium (TiN) layer presents a negative charge that favors increasing calcium ion deposition (positive charges), it is favorable for bone‐coating applications . In addition, plasma TiN surfaces reduce bacteria adhesion due to biofilm formation, reducing the risk of infection and/or bone implant rejection …”

Section: Introductionmentioning

confidence: 99%

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Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

et al. 2019

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A titanium surface nitrided by plasma contains nitrogen ions that guarantee resistance to corrosion and biocompatibility. Despite this, no descriptions concerning the influence of the expression of cell adhesion proteins and their influence on osteogenic cell differentiation are available. Thus, the present study aimed to assess the response of murine pre‐osteoblastic cells (MC3T3‐E1) cultured on nitrided titanium surfaces. Pre‐osteoblastic cells were grown on polished titanium discs, used as controls, and on previously characterized plasma‐nitrided titanium discs. Cells from both groups were submitted to the MTT cell viability test. The expressions of α5, α2, and β1 integrin were assessed by flow cytometry and immunofluorescence, while osteocalcin expression was assessed by flow cytometry. The nitrided surface presented higher α2 and β1 integrin expressions, as well as osteocalcin expression, when compared to the polished surface, with no alterations in cell viability. These findings seem to suggest that the plasma nitriding treatment produces a titanium surface with the potential for effective in vitro osseointegration.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

et al. 2019

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Novel antibacterial silver coating on PET fabric assisted with hollow‐cathode glow discharge

Naeem

Felipe

Medeiros

et al. 2020

Polymers for Advanced Techs

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The silver-coated fabrics are of much importance because of their outstanding antibacterial features and are useable in several medical and hygienic applications. The silver deposition on fabrics by conventional techniques is not feasible because of their high processing cost, long processing duration, complex equipment, and multiple steps processing (nanoparticle synthesis and subsequent deposition on fabrics). In this novel study, the antibacterial silver coating is deposited by using a hollow cathode discharge (HCD) capable of generating high-density plasma, and thus it exhibits high-efficiency processing. The silver is deposited on woven and non-woven PET fabrics for various treatment times (10-60 minutes), and their antibacterial performance against E. coli and S. aureus bacterial is tested. The XRD results verified the deposition of silver with (111) preferred orientation, while SEM analysis depicted the uniform/ homogeneous deposition of silver particles. The interfacial free energy of adhesion depicts that after the silver deposition on both fabrics, the surface is actively unfavorable for bacterial adhesion. The antibacterial test revealed that the silver-coated woven and non-woven PET fabrics exhibit exceptional antibacterial activity against E. coli and S. aureus bacteria. As the HCD technique is relatively cost-effective, no need for specific sputtering targets, eco-friendly, and require single-step processing for silver deposition. Thus the results are expected to be of remarkable importance to prepare silver-coated antibacterial fabrics useable in hospitals and other appropriate applications.

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Enhancing Titanium Disk Performance through In-Pack Cold Atmospheric Plasma Treatment

Martins,

da Silva Braz,

de Macedo

et al. 2024

ACS Biomater. Sci. Eng.

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While titanium dental implants have already been clinically established, ongoing research is continuously being conducted to advance the fields of osseointegration and bacterial resistance, seeking further improvements in these areas. In this study, we introduce an innovative method for treating titanium surfaces within tightly sealed packaging. Specifically, titanium discs, enclosed in surgical-grade packaging, underwent treatment using cold atmospheric plasma (CAP). The surfaces were thoroughly characterized in terms of wettability, crystalline structure, and chemical composition. Hemocompatibility analyses were conducted using blood diluted in sodium citrate (1:9) exposed to titanium discs for 30 min inside a CO 2 incubator at 37 °C. Subsequently, various blood parameters were evaluated, including prothrombin time (PT), activated partial thromboplastin time (APTT), and platelet adhesion. Microbiological analyses were also performed using Pseudomonas aeruginosa (ATCC 27853) for 4 h at 37 °C. The treatment with CAP Jet resulted in a reduction in contact angle without causing any changes in the crystalline structure. No statistically significant differences were observed in the blood parameters. The plasma-treated samples exhibited lower PT and APTT values compared to those of the control group. The surfaces treated with CAP Jet showed increased platelet activation, platelet density, and thrombus formation when compared with the untreated samples. Moreover, the treated surfaces demonstrated lower bacterial colony formation compared with other surfaces.

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Minimizing <em>Pseudomonas aeruginosa</em> adhesion to titanium surfaces by a plasma nitriding process

Cited by 5 publications

References 25 publications

Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

Novel antibacterial silver coating on PET fabric assisted with hollow‐cathode glow discharge

Enhancing Titanium Disk Performance through In-Pack Cold Atmospheric Plasma Treatment

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