Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

Abstract

A titanium surface nitrided by plasma contains nitrogen ions that guarantee resistance to corrosion and biocompatibility. Despite this, no descriptions concerning the influence of the expression of cell adhesion proteins and their influence on osteogenic cell differentiation are available. Thus, the present study aimed to assess the response of murine pre‐osteoblastic cells (MC3T3‐E1) cultured on nitrided titanium surfaces. Pre‐osteoblastic cells were grown on polished titanium discs, used as controls, and on previously characterized plasma‐nitrided titanium discs. Cells from both groups were submitted to the MTT cell viability test. The expressions of α5, α2, and β1 integrin were assessed by flow cytometry and immunofluorescence, while osteocalcin expression was assessed by flow cytometry. The nitrided surface presented higher α2 and β1 integrin expressions, as well as osteocalcin expression, when compared to the polished surface, with no alterations in cell viability. These findings seem to suggest that the plasma nitriding treatment produces a titanium surface with the potential for effective in vitro osseointegration.

Live endothelial cells on plasma-nitrided and oxidized titanium: An approach for evaluating biocompatibility

“…In this context, surface roughness and wettability are important parameters concerning cell integration processes, such as osseointegration [7]. However, to promote tissue repair in addition to bone integration, angiogenesis using endothelial cells (EC) is required [8].This process can trigger the activation of cell adhesion proteins (integrins), as well as rapid adhesion, proliferation and development of normal endothelial function [9,10]. Interestingly, titanium surface modifications, such as by argon plasma and Cl 2 /Ar plasma, allow for improvements in cellular response and the prevention of restenosis of intra-stents comprising this metal [11,12].…”

Biocompatible Gas Plasma Treatment Affects Secretion Profiles but Not Osteogenic Differentiation in Patient-Derived Mesenchymal Stromal Cells

“…Surfaces of biomaterials can be oxidatively modified by CPP treatment. This modification (dependent on plasma conditions) can positively affect cell behavior in terms of osteogenic capacity [36][37][38].…”

Two Gingival Cell Lines Response to Different Dental Implant Abutment Materials: An In Vitro Study

“…The materials investigated in this work were selected either as they are currently in use as abutments (Ti, ZrO 2 , and CoCr) [ 55 , 56 , 57 ] or were materials currently under investigation (TiN, and m-PEEK) [ 42 , 58 ] for providing better aesthetic alternatives, but with (potentially) comparable biological influence on the peri-implant hard and soft tissues.…”