Minimising plasma irradiation area by micronozzle device towards single-cell treatment

Shimane, R.; Kumagai, Shinya; Hori, Masaru; Sasaki, Minoru

doi:10.1049/mnl.2012.0555

Cited by 9 publications

(5 citation statements)

References 11 publications

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“…There are several methods to generate low-temperature plasma at atmosphere. In our previous reports using the MEMS nozzle, 23,24) the plasma source was driven by inductive coupling. The plasma source is one of the popular sources for generating high-density plasma.…”

Section: Microplasma Sourcementioning

confidence: 99%

“…23,24) The nozzle tips are attached to outlets of atmospheric pressure plasma sources. The size of nozzle holes, which is defined by the resolution of MEMS fabrication technology, determines the size of irradiation area.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, micro electromechanical systems (MEMS) technology has been applied to fabricate nozzle tips for plasma irradiation. 23,24) The nozzle tips are attached to outlets of atmospheric pressure plasma sources. The size of nozzle holes, which is defined by the resolution of MEMS fabrication technology, determines the size of irradiation area.…”

Section: Introductionmentioning

confidence: 99%

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Development of plasma-on-chip: Plasma treatment for individual cells cultured in media

Kumagai

Chang

Jeong

et al. 2015

Jpn. J. Appl. Phys.

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A device consisting of Si microwells and microplasma sources has been fabricated for plasma treatment of individual cells cultured in media. We named the device plasma-on-chip. The microwells have through-holes at the bottom where gas–liquid interfaces form when they are filled with media containing biological samples. The microplasma sources, which supply reactive species, are located on the back of each microwell. Through the gas–liquid interface, the reactive species are supplied to the cells. Chlorella cells were used to demonstrate the feasibility of the device and after three minutes of plasma treatment, the fluorescence intensity of Chlorella cells appeared to be decreased. Optical emission spectroscopy identified O and OH radicals in the plasma, which can affect the cells. In the analysis of biological samples such as human cells or tissues, this device raises the possibility of revealing the mechanisms of plasma medicine in more detail.

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Section: Microplasma Sourcementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of plasma-on-chip: Plasma treatment for individual cells cultured in media

Kumagai

Chang

Jeong

et al. 2015

Jpn. J. Appl. Phys.

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“…Thus far, we have fabricated a MEMS nozzle device to provide localized plasma irradiation onto a cell. 20) The device consists of nozzle holes and gas exhaust trenches. The cross-sectional area of the gas exhaust trench (136000 µm 2 ) was much larger than the total areas of the nozzle holes (19.6 µm 2 ).…”

Section: Introductionmentioning

confidence: 99%

Localized plasma irradiation through a micronozzle for individual cell treatment

Shimane

Kumagai

Hashizume

et al. 2014

Jpn. J. Appl. Phys.

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A micronozzle device was fabricated for the localized plasma treatment of a cell. The device was attached to the tips of two ϕ1.5 mm capillary tubes injecting and evacuating the discharging plasma gas. At the bottom of the channel where the discharging gas flows, nozzle holes (ϕ2%30 µm) were prepared. Controlling the injecting and evacuating gas flows made the pressure in the channel negative or positive relative to the atmosphere. The cells were trapped or released through the nozzle holes. When the cells were trapped, the nozzle hole also defined the area of plasma treatment. An atmospheric-pressure microplasma was generated (He: 0.3 L/min, power: 30 W) for localized treatment. The test specimen was a plant cell, lily pollen (length: 100-140 µm). No burning of the pollen was observed during the 10 min plasma treatment. Only part of the surface reacted with the plasma irradiation. The depth of removal was about 1.5 µm.

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“…It is readily understood that the plasma affects cells in the vicinity of the treated area. Figure 1 illustrates what occurs when the plasma irradiation area is reduced to a size as small as a cell (plasma ≒ cell) or smaller (plasma < cell) 30 31 32 33 34 . In these cases, we expect that the plasma treatment activates or inactivates biological reactions at the single-cell level.…”

mentioning

confidence: 99%

Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells

Kojima

Sasaki

et al. 2017

Sci Rep

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We have developed a plasma cell treatment device called Plasma-on-Chip that enables the real-time monitoring of a single cell culture during plasma treatment. The device consists of three parts: 1) microwells for cell culture, 2) a microplasma device for generating reactive oxygen and nitrogen species (RONS) for use in cell treatment, and 3) through-holes (microchannels) that connect each microwell with the microplasma region for RONS delivery. Here, we analysed the delivery of the RONS to the liquid culture medium stored in the microwells. We developed a simple experimental set-up using a microdevice and applied in situ ultraviolet absorption spectroscopy with high sensitivity for detecting RONS in liquid. The plasma-generated RONS were delivered into the liquid culture medium via the through-holes fabricated into the microdevice. The RONS concentrations were on the order of 10–100 μM depending on the size of the through-holes. In contrast, we found that the amount of dissolved oxygen was almost constant. To investigate the process of RONS generation, we numerically analysed the gas flow in the through-holes. We suggest that the circulating gas flow in the through-holes promotes the interaction between the plasma (ionised gas) and the liquid, resulting in enhanced RONS concentrations.

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Minimising plasma irradiation area by micronozzle device towards single-cell treatment

Cited by 9 publications

References 11 publications

Development of plasma-on-chip: Plasma treatment for individual cells cultured in media

Development of plasma-on-chip: Plasma treatment for individual cells cultured in media

Localized plasma irradiation through a micronozzle for individual cell treatment

Plasma cell treatment device Plasma-on-Chip: Monitoring plasma-generated reactive species in microwells

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