Minimal HER1 and HER2 expressions in CHO and HEK-293 cells cause them appropriate negative cells for HERs-related studies

Abstract

Background: Understanding of cellular and molecular mechanisms involved in cell/tumor growth and progression has led to molecular-targeted therapy. HER1 and HER2 are two main oncogenic components of HER-receptor family considered as targets for cancer research and therapy. Reliable results of HERrelated research and targeted therapies dependent upon cell line with known HER expression status. But there are no published studies focusing on determination of HER1 and HER2 receptors expression in CHO and HEK-293 cell lines. Materials and Methods: Absolute expressions of HER1 and HER2 in CHO, HEK-293, MDA-MB-468 and SKBR3 cells were evaluated using quantitative real-time PCR. Absolute expression quantification was carried out by serial dilution plasmids harboring HER1 and HER2 to extrapolate standard curve. Results: Real-time PCR amplification was successfully optimized by HER1 and HER2 plasmids with high efficiency and their copy numbers were calculated. The results showed that HER1 was not expressed in CHO and HEK-293 cells but these cells express HER2 up to 9.4 × 10 2 and 1

Development of a simple, sensitive and selective colorimetric aptasensor for the detection of cancer-derived exosomes

“…The HER2 ExoAptaSensor was found to be specific against HER2 expressing HCC1954 exosomes and the exosomes derived from PC cell line (P0403), epithelial cell line (HEK-293), lung cancer cell line (A549), colorectal cancer cell line (HCT116), fetal bovine serum (FBS), and healthy human plasma caused minimal inference (Figure 5B). This data agrees with reported HER2 expression level in P0403 (no), HEK-293 (minimal), A549 (low), HCT116 (low) cell line, and FBS (no) (LaBonte et al, 2011), (Hosseinzadeh Colagar et al, 2013, (Bunn et al, 2001), (LaBonte et al, 2011, (Barok et al, 2018). The calibration curve for HER2 detection was established by testing a range of HER2 + exosomes (y=0.2094x + 0.5857, R 2 =0.97948) (Figure 5C).…”

Water-Soluble Chlorin/Arylaminoquinazoline Conjugate for Photodynamic and Targeted Therapy

“…Cellular Uptake Study. The ability of compounds 1 and 6 to penetrate into and be accumulated by living cells was studied on cell lines with different expression levels of epidermal growth factor receptor (EGFR): Chinese hamster ovary (CHO) cells (EGFR-negative) 54 and A431 (human epidermoid carcinoma, high levels of EGFR expression). The level of the EGFR expression was experimentally proved by flow cytometry with immunofluorescence staining (Figure S19).…”

Endogenous Cell-Surface Receptor Modification by Metal Chelation-Assisted Pyridinium Oxime Catalyst

“…20,21 Finally, we performed selective visualization of endogenous HER2 in a mixed cell culture model containing N87 cells and HER2-negative HEK293T cells. 27 In this experiment, the cell surface of HEK293T cells was biotinylated with a biotin-NHS reagent and fluorescently marked with an Alexa647-streptavidin conjugate (SAv-647) to distinguish them from N87 cells. After the mixed cells were labeled with 2-scFv (1 μM) and 3-Ni (1 μM), confocal laser scanning microscopy (CLSM) imaging was conducted.…”