Development of a simple, sensitive and selective colorimetric aptasensor for the detection of cancer-derived exosomes

Xu, Lizhou; Chopdat, Raheemah; Li, Danyang; Al‐Jamal, Khuloud T.

doi:10.1016/j.bios.2020.112576

Cited by 75 publications

(71 citation statements)

References 35 publications

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“…In accordance with this finding, Xu et al (Fig. 3A) fabricated a colorimetric biosensor by introducing HRP to the surface of the exosomes and realized the detection within 10 min [111]. The sensitivity was further enhanced because the formed PDA could deposit around exosomes via the outstanding reactivity between PDA and amine, sulfhydryl, and the phenol groups of proteins [115].…”

Section: Colorimetric Biosensorsmentioning

confidence: 68%

“…Colorimetry exhibits attractive potential in biosensor construction due to its cost effectiveness, visual output, and simplicity. Colorimetric biosensors could transform the detection events into apparent color changes through two main approaches: distances or dispersion status change in nanomaterials and oxidation reactions mediated by enzymes [110,111]. Thus, nanomaterials and enzymes are commonly used as signal tags in colorimetric assay [112].…”

Section: Colorimetric Biosensorsmentioning

confidence: 99%

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Biosensor-based assay of exosome biomarker for early diagnosis of cancer

et al. 2021

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Cancer imposes a severe threat to people’s health and lives, thus pressing a huge medical and economic burden on individuals and communities. Therefore, early diagnosis of cancer is indispensable in the timely prevention and effective treatment for patients. Exosome has recently become an attractive cancer biomarker in noninvasive early diagnosis because of the unique physiology and pathology functions, which reflects remarkable information regarding the cancer microenvironment, and plays an important role in the occurrence and evolution of cancer. Meanwhile, biosensors have gained great attention for the detection of exosomes due to their superior properties, such as convenient operation, real-time readout, high sensitivity, and remarkable specificity, suggesting promising biomedical applications in the early diagnosis of cancer. In this review, the latest advances of biosensors regarding the assay of exosomes were summarized, and the superiorities of exosomes as markers for the early diagnosis of cancer were evaluated. Moreover, the recent challenges and further opportunities of developing effective biosensors for the early diagnosis of cancer were discussed.

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Section: Colorimetric Biosensorsmentioning

confidence: 68%

Section: Colorimetric Biosensorsmentioning

confidence: 99%

Biosensor-based assay of exosome biomarker for early diagnosis of cancer

et al. 2021

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“…Xu et al developed a highly sensitive colorimetric-based aptasensor for the detection of exosomes obtained from breast and pancreatic cancer cells. In this novel approach, the specific detection was accelerated by horseradish peroxidase (HRP) accelerated dopamine polymerization, and sensitivity was enhanced by in situ deposition of polydopamine around exosomes particles (26). Shayesteh et al developed a labelfree colorimetric aptasensor, using poly-adenine aptamer and gold nanoparticles for sensitive detection of prostate-specific antigen (PSA) tumor marker.…”

Section: Colorimetric Aptasensorsmentioning

confidence: 99%

Advances in Optical Aptasensors for Early Detection and Diagnosis of Various Cancer Types

Zahra

Khan

2021

Front. Oncol.

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Cancer is a life-threatening concern worldwide. Sensitive and early-stage diagnostics of different cancer types can make it possible for patients to get through the best available treatment options to combat this menace. Among several new detection methods, aptamer-based biosensors (aptasensors) have recently shown promising results in terms of sensitivity, identification, or detection of either cancerous cells or the associated biomarkers. In this mini-review, we have summarized the most recent (2016–2020) developments in different approaches belonging to optical aptasensor technologies being widely employed for their simple operation, sensitivity, and early cancer diagnostics. Finally, we shed some light on limitations, advantages, and current challenges of aptasensors in clinical diagnostics, and we elaborated on some future perspectives.

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“…A sensitive and selective colorimetric aptasensor was successfully implemented for the detection of cancer-derived exosomes. The chromogenic signal was produced by the polymerization of horseradish peroxidase (HRP)-accelerated dopamine (DA) and the in situ deposition of polydopamine (PDA) [77]. The target exosomes were first isolated by latex beads followed by bio-recognition using a specific CD63 aptamer, which was conjugated to horseradish peroxidase (HRP) through biotin-streptavidin binding [77] (Figure 4).…”

Section: Colorimetric Sensorsmentioning

confidence: 99%

“…The chromogenic signal was produced by the polymerization of horseradish peroxidase (HRP)-accelerated dopamine (DA) and the in situ deposition of polydopamine (PDA) [77]. The target exosomes were first isolated by latex beads followed by bio-recognition using a specific CD63 aptamer, which was conjugated to horseradish peroxidase (HRP) through biotin-streptavidin binding [77] (Figure 4). Colorimetric detection was completed in 10 min via enzymatic catalysis, which produced dark-colored polydopamine (PDA) from colorless dopamine (DA).…”

Section: Colorimetric Sensorsmentioning

confidence: 99%

Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles

Bari

Hossain

Nestorova

2021

Sensors

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Exosomes are extracellular vehicles (EVs) that encapsulate genomic and proteomic material from the cell of origin that can be used as biomarkers for non-invasive disease diagnostics in point of care settings. The efficient and accurate detection, quantification, and molecular profiling of exosomes are crucial for the accurate identification of disease biomarkers. Conventional isolation methods, while well-established, provide the co-purification of proteins and other types of EVs. Exosome purification, characterization, and OMICS analysis are performed separately, which increases the complexity, duration, and cost of the process. Due to these constraints, the point-of-care and personalized analysis of exosomes are limited in clinical settings. Lab-on-a-chip biosensing has enabled the integration of isolation and characterization processes in a single platform. The presented review discusses recent advancements in biosensing technology for the separation and detection of exosomes. Fluorescent, colorimetric, electrochemical, magnetic, and surface plasmon resonance technologies have been developed for the quantification of exosomes in biological fluids. Size-exclusion filtration, immunoaffinity, electroactive, and acoustic-fluid-based technologies were successfully applied for the on-chip isolation of exosomes. The advancement of biosensing technology for the detection of exosomes provides better sensitivity and a reduced signal-to-noise ratio. The key challenge for the integration of clinical settings remains the lack of capabilities for on-chip genomic and proteomic analysis.

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Development of a simple, sensitive and selective colorimetric aptasensor for the detection of cancer-derived exosomes

Cited by 75 publications

References 35 publications

Biosensor-based assay of exosome biomarker for early diagnosis of cancer

Biosensor-based assay of exosome biomarker for early diagnosis of cancer

Advances in Optical Aptasensors for Early Detection and Diagnosis of Various Cancer Types

Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles

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