Miniaturized fiber-in-tube solid-phase extraction as the sample preconcentration method for microcolumn liquid-phase separations

Abstract

Miniaturized fiber-in-tube solid-phase extraction (fiber-in-tube SPE) has been developed as a solventless sample preconcentration technique for microcolumn liquid-phase separation methods. Short capillaries packed with polymer filaments were employed as the extraction tube and the preconcentration power for phthalates in aqueous solutions was studied. On the basis of the successful on-line coupling of this preconcentration method with liquid chromatography (LC), a more miniaturized extraction cartridge, which is installed in the rotor of the micro-injector, has been developed. With a modified commercially available valve, on-line coupling of this sample preconcentration method to capillary electrochromatography (CEC) was also investigated.

Miniaturized Sample Preparation and Separation Methods for Environmental and Drug Analyses

“…Figure 6 shows the illustration of three different types of the extraction tube: (a) in-tube, (b) wirein-tube, 17 and (c) fiber-in-tube. [18][19][20][21][22] A typical chromatogram for the analysis of the phthalates with in-tube ( Fig. 6 (a)) SPME preconcentration is shown in Fig.…”

“…As an extension of the studies described above, a miniaturized fiber-in-tube extraction cartridge is introduced as a solventless sample preconcentration technique for microcolumn liquid-phase separations, such as micro-LC, CE and CEC. 20 To prepare the extraction tube (cartridge), a short capillary of PEEK or polytetrafluoroethylene (PTFE) was packed longitudinally with fine polymer filaments (Zylon ® ) having both solvent resistance and mechanical strength.…”

“…In this review, the authors described some recent results, especially for microscale sample preparation techniques with novel fibrous materials, and the coupling of these methods to microcolumn separation methods. The authors believe that these fiber-packed media [18][19][20][21][22][34][35][36][37][38][39] must be used routinely along with the precisely controlled analytical devices and/or systems in the near future.…”

High-Temperature Separations on a Polymer-Coated Fibrous Stationary Phase in Microcolumn Liquid Chromatography

“…[9][10][11][12][13][14] By introducing fine fibrous materials as the extraction medium, several novel microscale sample preparation techniques were developed based on excellent resistance to typical organic solvents. [15][16][17][18][19][20][21][22] These fibrous materials were also employed as a stationary phase in GC, because of their excellent thermal stability over the temperature range typically used in most of GC separations. [23][24][25][26][27] Coated with typical polysiloxane-based materials onto the surface of the fibrous materials, the retentivity was significantly improved, and also the selectivity could be designed on the basis of the type of polymeric coatings.…”

Rapid Temperature-Programmed Separation and Retention Prediction on a Novel Packed-Capillary Column in Gas Chromatography

“…[8][9][10] Packed with a bundle of filaments having a polymeric coating material thereon, a parallel alignment of these filaments was formed in a short capillary, and the resulting polymer-coated fiber-packed columns showed a satisfactory separation performance with a fast temperature-programming rate in GC. 11,12 It has been demonstrated that the use of a narrower capillary allows these columns to be operated at a fast temperature-programming rate, as compared with conventional particle-packed columns.…”