High-Temperature Separations on a Polymer-Coated Fibrous Stationary Phase in Microcolumn Liquid Chromatography

Abstract

Novel polymer-coated fiber-packed microcolumns in liquid chromatography (LC) have been developed. Typical polymeric materials, such as polydimethylsiloxane and polyethyleneglycol, which are conventional stationary phases of capillary columns in gas chromatography (GC), have been employed as coating materials onto the surface of fine filaments. Packed longitudinally with a bundle of polymer-coated filaments into a stainless-steel capillary of 0.8 mm i.d., 150 mm length, several types of polymer-coated fiber-packed columns were prepared, and the retention behavior of aromatic compounds on these columns has been studied. A good linear relationship was obtained for van't Hoff plots over the temperature range between 0 and 200 C, clearly indicating an excellent heat-resistant property of these polymer-coated fibrous stationary phases. Taking advantage of the heat-resistant feature of the fibrous stationary phases, the separation of several test mixtures with temperature-programmed elution was studied, where a solvent gradient program was additionally introduced if needed. Separation was also carried out with pure water as the mobile phase using an appropriate temperature program.

Separation of Triglycerides in Oils and Fats by Comprehensive Two-Dimensional Liquid Chromatography and the Determination of the Fatty Acid Composition in Gas Chromatography

“…Further applications of the developed 2D-LC system are expected, especially for a high performance separation of other complex samples such as sardine and salmon oils, and also for a more effective separation when introducing appropriate sample preparation techniques [38][39][40][41][42][43][44] before the separations.…”

Miniaturization for the Development of High Performance Separation Systems

“…Miniaturized sample preparation with fiber-packed capillary and the effective coupling to microscale separation methods will suggest a future possibility to other related applications, such as an interface between two-dimensional chromatographic separations [101][102][103][104][105]. The applications of polymer-coated and surface-derivatized fibrous materials in miniaturized sample preparation process also demonstrate a possibility to the employment of similar fibrous separation media as the new format of the stationary phase materials in various chromatographic techniques.…”

Preconcentration of Aromatic Compounds in Aqueous Samples with a Polymer-coated Fiber-packed Capillary and Subsequent Temperature-programmed Elution with Water for Pseudo-2D LC Separations

“…The bundle of Zylon originally consisted of 166 fine filaments, and the number of the filaments per bundle was adjusted as described below. On the basis of preliminary results, 25,26 an HR-17 (50%-phenyl-50%-methylpolysiloxane, Shinwa Chemical Industries, Kyoto, Japan) was employed as the material of polymer-coating onto Zylon filaments.…”