Separation of Triglycerides in Oils and Fats by Comprehensive Two-Dimensional Liquid Chromatography and the Determination of the Fatty Acid Composition in Gas Chromatography

Abstract

An on-line coupled comprehensive two-dimensional liquid chromatography (2D-LC) system was developed for the separation of triglycerides (TGs) in this work. Taking advantage of two-dimensional separation on the basis of two separation columns with different selectivities, where an silver-ion column and an octylsilica column were employed as the columns in the 1st and 2nd dimensions, respectively, all the TGs in perilla and tuna oils were well separated. Introducing the systematic qualitative analysis in gas chromatography (GC), it was possible to make the assignments for all the fatty acid compositions in the TGs that were fractionated after the comprehensive 2D-LC separations. In the subsequent GC analysis, 23 and 76 types of TGs were determined for perilla and tuna oils, respectively. The results clearly showed an excellent performance of the two-dimensional separation system for the separation of TGs in oils and fats, suggesting a future applications to the high performance separation of other types of fish oils with the on-line coupled comprehensive 2D-LC system developed in this work.

Retention Behavior of Various Aromatic Compounds on Poly(butylene terephthalate) Stationary Phase in Liquid Chromatography

“…Liquid chromatography (LC) is one of the most widely used methods for the effective separation and isolation of various complex mixtures, because of the powerful separation capability and the applicability to wide variety of sample matrices [1][2][3][4]. Therefore, LC has been employed in various scientific fields, such as pharmaceutical, environmental and biomedical analyses as well as routine analytical situations [4][5][6][7][8].…”

Fibrous Polyimide Stationary Phase for the Separation of Aromatic Compounds in Reversed-Phase Liquid Chromatography

“…The chromatographic method enables the purification of a target analyte from a complex sample matrix and qualitative and quantitative analyses. Therefore, LC separation technique has been conventionally employed in a wide range of fields such as biomedical, pharmaceutical, and environmental science [1][2][3][4][5][6][7]. In order to achieve effective separation for various types of complex sample mixtures in LC measurements, appropriate separation conditions must be chosen.…”

Molecular Shape Selectivity for Polycyclic Aromatic Compounds on a Poly(benzoguanamine-<i>co</i>-melamine-<i>co</i>-formaldehyde) Stationary Phase in Reversed-Phase Liquid Chromatography

“…Liquid chromatography (LC) is one of the most powerful and convenient separation methods, especially for the separation of non-volatile sample matrix in separation science [1][2][3][4]. In order to obtain an effective separation of the complex sample mixtures commonly encountered in actual separation problems, the stationary phase plays an essential role [5][6][7][8]. Development of novel stationary phases has been regarded as a key process to create more efficient and cost-effective separations.…”