Miniaturisation in pressure and electroendosmotically driven liquid chromatography: Some theoretical considerations

Knox, John H.; GRANT, I

doi:10.1007/bf02688476

Cited by 568 publications

(276 citation statements)

References 25 publications

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“…As would be expected, the increase of pH resulted in an increase in EOF velocity. The relationship between the EOF velocity and pH is consistent with the theory of EOF generation [23,24]. If pH decreases, the protonation of the silanol groups on the capillary wall and packing materials increases and the EOF velocity is reduced.…”

Section: Effect Of Ph In the Mobile Phasesupporting

confidence: 84%

Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection

Liu¹,

Zhang²,

Lin³

et al. 2007

Electrophoresis

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A new analytical method, pressurized CEC (pCEC) with amperometric detection (AD) using 1.5 mm RP nonporous silica packed columns has been developed for the rapid separation and determination of four Sudan dyes in hot chilli. The influence of several experimental parameters on the retention behavior has been investigated. The electrochemical oxidation of Sudans I-IV separated by pCEC can be reliably monitored with a carbon electrode at 10.95 V (vs. Ag/AgCl). Fast and efficient separation of the analytes was achieved within 7 min by pCEC under the optimum conditions with an ACN/water (95:5%) mobile phase containing formic acid (pH 4.3), 5% acetone and 0.002% triethylamine using a separation voltage of 12 kV. The detection limits for four Sudan dyes ranged from 8.0 6 10 27 to 1.2 6 10 26 mol/L. To evaluate the feasibility and reliability of this method, the proposed pCEC-AD method was further demonstrated with hot chilli samples spiked with Sudan dyes.

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Section: Effect Of Ph In the Mobile Phasesupporting

confidence: 84%

Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection

Liu¹,

Zhang²,

Lin³

et al. 2007

Electrophoresis

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show abstract

“…In order to obtain the equations that relate the separation efficiency to the thermal effect for both, cylindrical and rectangular columns, we have employed the equation by Virtanen [22] as also derived by Knox and Grant [23]. For cylindrical capillaries they applied a modification of the Taylor …”

Section: Efficiencymentioning

confidence: 99%

Rectangular capillary electrophoresis: Some theoretical considerations

Cifuentes

Poppe

1994

Chromatographia

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SummaryA theoretical study of the use of rectangular columns in capillary electrophoresis (CE) is presented. It was done employing some of the most important parameters that normally define the performance of CE separations, i.e. thermal effect, analysis speed, efficiency and sample capacity. Theoretical results from rectangular and cylindrical capillaries are compared in terms of the aforementionated parameters. Also, an estimate of the additional zone broadening that arises from the noninfinite dimension in the y-direction, in which the channel has its largest dimension, is presented.

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“…A pressurized capillary electrochromatography (pCEC) is a relatively new microcolumn separation technique, which combines the advantages of HPLC and capillary electrochromatography (CE) (Jorgenson & Lukacs, 1981;Pretorius & Hopkins, 1974;Knox & Grant, 1987). By introducing high pressure on the electrochromatographic separation, pCEC combines the benefit of an electroosmotic flow (EOF) which could increase peak capacity and shorten analysis time and the supplementary which could suppress bubble formation (Tsuda, 1988).…”

Section: Guanhuangbaimentioning

confidence: 99%

A comparative study on analytical method of total alkaloids from cortex Phellodendri amurens by reversed phase high performance liquid chromatography (RP-HPLC) and pressurized capillary electrochromatography (pCEC)

Yang¹,

Zhang²,

Zhang³

2011

Rev. bras. farmacogn.

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Abstract:A pressurized capillary electrochromatography (pCEC) method with post-column detection cell has been developed for the analysis of total alkaloids of cortex Phellodendron amurense Rupr., Rutaceae. The separation of total alkaloids (berberine, palmatine, oatrorrhizine, magnoflorine, phellodendrine, candicine, menisperine) was optimized by compositions of the mobile phase, ionic strength of buffers, pH value, and applied voltage. Separation of total alkaloids was achieved within 11 min by using a mobile phase of Na 2 HPO 4 -citric acid solution-acetonitrile (pH 4.00; 3 mM) (60:40, v/v) and applying a voltage of -10 kV. This method showed satisfactory retention times and peak shapes. Meanwhile, a reversed phase high performance liquid chromatography (RP-HPLC) has also been established for the separation of total alkaloids extracted from cortex Phellodendri amurens. Baseline separation of total alkaloids was achieved within 25 min by using a mobile phase of acetonitrile-0.1% phosphoric acid with 0

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Miniaturisation in pressure and electroendosmotically driven liquid chromatography: Some theoretical considerations

Cited by 568 publications

References 25 publications

Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection

Development of a new method for analysis of Sudan dyes by pressurized CEC with amperometric detection

Rectangular capillary electrophoresis: Some theoretical considerations

A comparative study on analytical method of total alkaloids from cortex Phellodendri amurens by reversed phase high performance liquid chromatography (RP-HPLC) and pressurized capillary electrochromatography (pCEC)

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