Miniature Swine Breeds used Worldwide in Research

Panepinto, L M

doi:10.1007/978-1-4615-5885-9_24

Cited by 9 publications

(16 citation statements)

References 11 publications

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“…Ten different haplotypes were detected, three from SNU miniature pigs, five from KNP, and two from NIH miniature pigs (Table 4). Haplotype DQB1*0201/DRB1*0201 was present in both SNU and NIH breeds, which is consistent with the fact that both of these inbred pigs originated from Minnesota miniature pigs (25, 36). When we compared the haplotypes of SLA class II with those reported in the IPD, three new haplotypes, 0701/0404, 040101/kn04, and 0601/1001, were found.…”

Section: Resultssupporting

confidence: 81%

Systematic analysis of swine leukocyte antigen‐DRB1 nucleotide polymorphisms using genomic DNA‐based high‐resolution genotyping and identification of new alleles

et al. 2011

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In an attempt to enable comprehensive high-resolution genotyping of the swine leukocyte antigen (SLA) gene, we performed a systemic analysis of nucleotide polymorphisms at introns 1 and 2 and exon 2 from diverse alleles of SLA-DRB1 and DRB1 pseudogenes. We amplified and cloned 16 partial sequences of SLA-DRB1 and DRB2 introns 1 and 2 from different alleles, and analyzed them together with sequences of four reported SLA-DRB pseudogenes, DRB2, 3, 4, and 5. The results showed the presence of extreme nucleotide variations within introns 1 and 2 of SLA-DRB-related genes including substitutions and deletions. On the basis of these results, we developed a comprehensive genotyping method for SLA-DRB1 by genomic polymerase chain reaction (PCR) and subsequent direct sequencing. A total of 415 animals were genotyped and 67 allelic combinations from 18 DRB1 alleles were identified. Among them, two alleles, SLA-DRB1*kn04 and *kn05, were previously unreported. SLA-DRB1 genotyping results from this study combined with those of SLA-DQB1 from our previous study presented 10 SLA class II haplotypes, three of which were previously unreported. Population analysis using seven different pig breeds showed differences in the allele frequency of SLA-DRB1 among breeds. Our results should benefit biological experiments requiring sequence-level genotyping results of SLA-DRB1 and further study of the complete genetic diversity of SLA-DRB1 using field samples.

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Section: Resultssupporting

confidence: 81%

Systematic analysis of swine leukocyte antigen‐DRB1 nucleotide polymorphisms using genomic DNA‐based high‐resolution genotyping and identification of new alleles

et al. 2011

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“…A Yorkshire domestic pig was later introduced into the strain. Significant characterization and development took place later at the Sinclair Comparative Medicine Research Farm at the University of Missouri (Panepinto 1996). Researchers at the University of Missouri have used alloxan (175 mg/kg intravenously)-induced diabetic Sinclair minipigs to examine the relationship of diabetes to vascular dysfunction.…”

Section: Sinclair Minipigsmentioning

confidence: 99%

“…Its early development and characterization for laboratory use took place at Colorado State University (Panepinto 1996). Selective breeding has been reported to be a successful strategy for producing pigs with impaired glucose tolerance.…”

Section: Yucatan Minipigsmentioning

confidence: 99%

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Swine Models of Type 2 Diabetes Mellitus: Insulin Resistance, Glucose Tolerance, and Cardiovascular Complications

Bellinger¹,

Merricks²,

Nichols³

2006

ILAR Journal

146

110

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In humans, diabetes mellitus (DM) is considered a heterogeneous metabolic disorder. Swine have been used as a model for many human conditions including type 1 (insulindeficient) and type 2 (insulin-resistant) DM research because of their phenotypic similarities to humans including: cardiovascular anatomy and function, metabolism, lipoprotein profile, size, tendency to obesity, and omnivorous habits. There is phenotypic overlap between the two types of DM and pig models show characteristics and complications of both. Streptozotocin and alloxan have been used to create insulin deficient diabetes in pigs. One of the most unique and useful phenotypes is that these insulin-deficient pigs develop more severe coronary atherosclerosis than nondiabetic controls. It is not fully understood why patients with either type 1 or type 2 DM have increased severity and diffuseness of atherosclerosis compared with nondiabetic patients. The current human epidemic of type 2 DM and its attendant cardiovascular complications underscore the unmet need for creating a useful, readily available animal model of type 2 insulin resistant DM that also develops coronary artery atherosclerosis. The phenotypic susceptibility to coronary atherosclerosis makes pigs an attractive species to identify causative mechanisms. Suggested criteria for validation of an animal model of humanoid type 2 DM are described. The goal would be to develop a useful animal model for mechanistic studies as well as to develop and test novel therapeutics both for type 2 DM as well as its cardiovascular complications.

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“…5 The number of breeds actually used in toxicity testing is even smaller ( Table 1). Requirement for large homogenous groups, availability of extensive background data, and good genetic management of breeding stock are key reasons for this; furthermore, Association for Assessment and Accreditation of Laboratory Animal Care International accreditation is also oftentimes demanded, and only few breeds meet all these criteria.…”

Section: Minipigs Breedsmentioning

confidence: 98%

The Minipig as Nonrodent Species in Toxicology—Where Are We Now?

Ganderup¹,

Harvey

Mortensen³

et al. 2012

Int J Toxicol

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Over the past 3 decades minipigs have moved from being an obscure alternative to dogs and nonhuman primates to being a standard animal model in regulatory toxicity studies. This article covers the use of minipigs as a model in the context of nonclinical drug safety and provides an overview of the minipig's developmental history and relates minipigs to other animal species commonly used in toxicology; and the minipig's translational power is supported by 43 case studies of marketed drug products covered. Special focus is given to criteria for selecting minipigs in nonclinical programs supporting the development of new medicines; the use of swine in the assessment of food additives, agrochemicals, and pesticides; as well as a regulatory perspective on the use of minipigs in Food and Drug Administration (FDA)-regulated products. This article presents the main points conveyed at a symposium held at the 2010 American College of Toxicology meeting in Baltimore, Maryland.

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Miniature Swine Breeds used Worldwide in Research

Cited by 9 publications

References 11 publications

Systematic analysis of swine leukocyte antigen‐DRB1 nucleotide polymorphisms using genomic DNA‐based high‐resolution genotyping and identification of new alleles

Systematic analysis of swine leukocyte antigen‐DRB1 nucleotide polymorphisms using genomic DNA‐based high‐resolution genotyping and identification of new alleles

Swine Models of Type 2 Diabetes Mellitus: Insulin Resistance, Glucose Tolerance, and Cardiovascular Complications

The Minipig as Nonrodent Species in Toxicology—Where Are We Now?

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