“…Moreover, because of its adverse effects on water breeding products, several methods have been developed to detect MG. These include techniques such as adsorptive stripping voltammetry [ 4 ], high-performance liquid chromatography (HPLC) [ 5 ], immunoassays [ 6 ], mini-column extraction [ 7 ], molecular imprinting [ 8 , 9 ], and surface-enhanced Raman spectroscopy (SERS) [ 10 ]. In a study by Sun [ 11 ], a PMMA (polymethyl methacrylate)/Ag/graphene/Ag/graphene substrate was used to detect MG with a sensitivity of 10 −7 M. Moreover, Kumar et al [ 10 ] prepared a Ag-coated PDMS SERS substrate using taro leaf as a template, achieving a very low MG detection limit of 10 −11 M, which could be attributed to the high local field produced by the Ag granular film and nanovoids on the PDMS surface.…”