Mineralized human primary osteoblast matrices as a model system to analyse interactions of prostate cancer cells with the bone microenvironment

Reichert, Johannes C.; Quent, Verena M.C.; Burke, Les J.; Stansfield, Scott H.; Clements, Judith A.; Hutmacher, Dietmar W.

doi:10.1016/j.biomaterials.2010.06.055

Cited by 101 publications

(91 citation statements)

References 48 publications

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“…These results were consistent with the findings of Reichert et al (2010), who also identified growth factors in their decellularized matrices formed by primary human osteoblasts on a polymeric substrate. Notably, the growth factor concentrations obtained in our study were approximately 10 times higher than those reported by Reichert et al (2010). This may be due to differences in methodology, since Reichert et al (2010) used a different cell type and culture method, and did not use perfusion or DNAse.…”

Section: Discussionsupporting

confidence: 92%

“…Notably, the growth factor concentrations obtained in our study were approximately 10 times higher than those reported by Reichert et al (2010). This may be due to differences in methodology, since Reichert et al (2010) used a different cell type and culture method, and did not use perfusion or DNAse. The amounts of growth factors remaining in the cell sheet (range, 10-100 pg/mL for FGF-2 and VEGF, and 100-1,000 pg/mL for HGF) are likely to be of physiological relevance, since they are consistent with the reported serum concentrations of these growth factors (Veselý et al, 2004;Etto et al, 2008).…”

Section: Discussioncontrasting

confidence: 62%

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Decellularized Periodontal Ligament Cell Sheets with Recellularization Potential

et al. 2014

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The periodontal ligament is the key tissue facilitating periodontal regeneration. This study aimed to fabricate decellularized human periodontal ligament cell sheets for subsequent periodontal tissue engineering applications. The decellularization protocol involved the transfer of intact human periodontal ligament cell sheets onto melt electrospun polycaprolactone membranes and subsequent bi-directional perfusion with NH 4 OH/Triton X-100 and DNase solutions. The protocol was shown to remove 92% of DNA content. The structural integrity of the decellularized cell sheets was confirmed by a collagen quantification assay, immunostaining of human collagen type I and fibronectin, and scanning electron microscopy. ELISA was used to demonstrate the presence of residual basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and hepatocyte growth factor (HGF) in the decellularized cell sheet constructs. The decellularized cell sheets were shown to have the ability to support recellularization by allogenic human periodontal ligament cells. This study describes the fabrication of decellularized periodontal ligament cell sheets that retain an intact extracellular matrix and resident growth factors and can support repopulation by allogenic cells. The decellularized hPDL cell sheet concept has the potential to be utilized in future "off-theshelf " periodontal tissue engineering strategies.

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Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 62%

Decellularized Periodontal Ligament Cell Sheets with Recellularization Potential

et al. 2014

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“…For instance, before effective vascularization, the tumor growth appears to be closely reproduced in 3D culture systems, and the proliferation of tumor cells is typically slower and hence more indicative of physiological growth than that in monolayer cultures. To bridge the gap between 2D studies and in vivo animal models, the 3D culture of cancer cells in vitro has been presented in several recent studies [2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

3D polylactide-based scaffolds for studying human hepatocarcinoma processesin vitro

Scaffaro

Rigogliuso

et al. 2012

Science and Technology of Advanced Materials

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We evaluated the combination of leaching techniques and melt blending of polymers and particles for the preparation of highly interconnected three-dimensional polymeric porous scaffolds for in vitro studies of human hepatocarcinoma processes. More specifically, sodium chloride and poly(ethylene glycol) (PEG) were used as water-soluble porogens to form porous and solvent-free poly(L,D-lactide) (PLA)-based scaffolds. Several characterization techniques, including porosimetry, image analysis and thermogravimetry, were combined to improve the reliability of measurements and mapping of the size, distribution and microarchitecture of pores. We also investigated the effect of processing, in PLA-based blends, on the simultaneous bulk/surface modifications and pore architectures in the scaffolds, and assessed the effects on human hepatocarcinoma viability and cell adhesion. The influence of PEG molecular weight on the scaffold morphology and cell viability and adhesion were also investigated. Morphological studies indicated that it was possible to obtain scaffolds with well-interconnected pores of assorted sizes. The analysis confirmed that SK-Hep1 cells adhered well to the polymeric support and emitted surface protrusions necessary to grow and differentiate three-dimensional systems. PEGs with higher molecular weight showed the best results in terms of cell adhesion and viability.

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“…Primary hOBs were isolated from 3 individual donors (2 males and 1 female) undergoing knee or hip replacement as described previously [26] and approved by the Human Research Ethics Committees (HREC/14/QPCH/186, QUT/1400001024). HOBs were grown in αMEM (Life Technologies, Mulgrave, VIC, Australia), supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and used at passages 2-7.…”

Section: Cell Culturementioning

confidence: 99%

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

et al. 2017

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Endosteal-like extracellular matrix expression on melt electrospun written scaffolds, Acta Biomaterialia (2016), doi: http://dx.doi.org/10. 1016/j.actbio.2016.12.040 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. models that mimic the endosteal microenvironment enable researchers to discover the causes and improve treatments for blood and immune-related diseases. The aim of this study was to establish a physiologically relevant in vitro model using 3D printed scaffolds to assess the contribution of human cells to the formation of a construct that mimics human endosteum. Melt electrospun written scaffolds were used to compare the suitability of primary human osteoblasts (hOBs) and placenta-derived mesenchymal stem cells (plMSCs) in ( when compared to HSCs maintained using tissue culture plastic. This 3D testing platform represents an endosteal bone-like platform and warrants future investigation for the maintenance and expansion of human HSCs.

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Mineralized human primary osteoblast matrices as a model system to analyse interactions of prostate cancer cells with the bone microenvironment

Cited by 101 publications

References 48 publications

Decellularized Periodontal Ligament Cell Sheets with Recellularization Potential

Decellularized Periodontal Ligament Cell Sheets with Recellularization Potential

3D polylactide-based scaffolds for studying human hepatocarcinoma processesin vitro

Endosteal-like extracellular matrix expression on melt electrospun written scaffolds

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