Mineralization of flagella for nanotube formation

Hesse, William R.; Luo, Langli; Zhang, Guangneng; Mulero, Rafael; Cho, Junghyun; Kim, Min Jun

doi:10.1016/j.msec.2009.05.018

Cited by 21 publications

(25 citation statements)

References 28 publications

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“…It has also been shown that translocation behavior of proteins through solid‐state nanopores changes with the solution pH . We isolated the bacterial flagella and depolymerized the long filamentous structure into small seed particles of globular shape . The zeta potential of these protein subunits was −7.15 mV, suggesting that their presence can alter the electrokinetic behavior of bacteria.…”

Section: Resultsmentioning

confidence: 99%

Low aspect ratio micropores for single‐particle and single‐cell analysis

et al. 2015

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This paper describes microparticle and bacterial translocation studies using low aspect ratio solid-state micropores. Micropores, 5 μm in diameter, were fabricated in 200 nm thick free-standing silicon nitride membranes, resulting in pores with an extremely low aspect ratio, nominally 0.04. For microparticle translocation experiments, sulfonated polystyrene microparticles and magnetic microbeads in size range of 1-4 μm were used. Using the microparticle translocation characteristics, we find that particle translocations result in a change only in the pore's geometrical resistance while the access resistance remains constant. Furthermore, we demonstrate the ability of our micropore to probe high-resolution shape information of translocating analytes using concatenated magnetic microspheres. Distinct current drop peaks were observed for each microsphere of the multibead architecture. For bacterial translocation experiments, nonflagellated Escherichia coli (strain HCB 5) and wild type flagellated Salmonella typhimurium (strain SJW1103) were used. Distinct current signatures for the two bacteria were obtained and this difference in translocation behavior was attributed to different surface protein distributions on the bacteria. Our findings may help in developing low aspect ratio pores for high-resolution microparticle characterization and single-cell analysis.

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Section: Resultsmentioning

confidence: 99%

Low aspect ratio micropores for single‐particle and single‐cell analysis

et al. 2015

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“…Unlike nanopores, the use of tubular bacterial nanostructures in engineered systems is still largely in its incipient form. Much of the work that has been reported on bacterial nanotubes involves the modification of the structure via genetic means13–17 and with materials that are of engineering interest 8–12. One distinct advantage of protein‐based nanotubes is that they self‐assemble into geometries with highly repeatable dimensions.…”

Section: Nanotubesmentioning

confidence: 99%

“…As stated above, bacteria are useful to humanity in a plethora of ways but, relatively recently, proteins synthesized by bacteria play a unique role in nanofluidics and biotechnology. Such proteins include α ‐hemolysin,5, 6 flagellin,7–14 and many others 15–17. Nanoscale science and biology have a mutually beneficial effect on each other, where nanotechnology allows for the interrogation of biological phenomena with precision that was not previously possible, while biology gives inspiration and materials for future nanotechnologies.…”

Section: Introductionmentioning

confidence: 99%

“…Each of these structures are polymers of thousands of protein monomers that extend away from the cell body in vivo. The ability for genetic modification and the use of novel chemical methods have led to the use of these bacterial nanotubes as scaffolds for a wide variety of materials such as oxides,8, 9 conducting polymers,9 quantum dots,11 and metals 12, 14. In addition, bacterial proteins that are not natively filament forming, such as Hcp116 and trp‐RNA binding protein from Bacillus stearothermophilus ,17 have been engineered to form tubular filaments that are highly reproducible and amenable to modification 16, 17.…”

Section: Introductionmentioning

confidence: 99%

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Structural Requirements for the Antiproliferative Activity of Pre‐mRNA Splicing Inhibitor FR901464

Osman

Albert

Wang

et al. 2010

Chemistry A European J

104

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FR901464 is a natural product isolated from a bacterium source that activates a reporter gene, inhibits pre-mRNA splicing, and shows antitumor activity. We previously reported the development of a more potent analogue, meayamycin, through the total synthesis of FR901464. Herein, we report detailed structure-activity relationships of FR901464 that revealed the significance of the epoxide, carbon atoms in the left tetrahydropyran ring, the Z-geometry of the side chain, the 1,3-diene moiety, the C-4 hydroxy group, and the C2"-carbonyl group. Importantly, the methyl group of the acetyl substituent was found to be inessential, leading to a new potent analogue. Additionally, partially based on in vivo data, we synthesized and evaluated potentially more metabolically stable analogues for their antiproliferative activity. These structural insights into FR901464 may contribute to the simplification of the natural product for further drug development.

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“…Biologically derived materials are claimed to be more advantageous over other artificial materials due to the reputed simplicity of chemically manipulating the synthesis and mass production of such materials. Recently, we demonstrated that bacterial flagella can be used as potential bio-templates because of their unique features such as small diameter (inner diameter is 2 nm and outer diameter is approximately 20 nm) and long length, their unique tubular structure, and their robustness in harsh conditions [1,2]. The flagella-template allowed the generation of silica-mineralized flagella nanotubes (SMFNs) in an efficient manner by a well-controlled hydrolysis and condensation reaction.…”

Section: Introductionmentioning

confidence: 99%