Mineralization of Calcium Carbonate on Multifunctional Peptide Assembly Acting as Mineral Source Supplier and Template

Abstract

Crystal phase and morphology of biominerals may be precisely regulated by controlled nucleation and selective crystal growth through biomineralization on organic templates such as a protein. We herein propose new control factors of selective crystal growth by the biomineralization process. In this study, a designed β-sheet Ac-VHVEVS-CONH2 peptide was used as a multifunctional template that acted as mineral source supplier and having crystal phase control ability of calcium carbonate (CaCO3) during a self-supplied mineralization. The peptides formed three-dimensional nanofiber networks composed of assembled bilayer β-sheets. The assembly hydrolyzed urea molecules to one carbonate anion and two ammonium cations owing to a charge relay effect between His and Ser residues under mild conditions. CaCO3 was selectively mineralized on the peptide assembly using the generated carbonate anions on the template. Morphology of the obtained CaCO3 was fiber-like structure, similar to that of the peptide template. The mineralized CaCO3 on the peptide template had aragonite phase. This implies that CaCO3 nuclei, generated using the carbonate anions produced by the hydrolysis of urea on the surface of the peptide assembly, preferentially grew into aragonite phase, the growth axis of which aligned parallel to the direction of the β-sheet fiber axis.

Design, fabrication, and biomedical applications of bioinspired peptide–inorganic nanomaterial hybrids

“…31 As shown in Fig. 3b, the peptide motif KLVFF (Aβ [16][17][18][19][20] self-assembled into 2D membranes under the influence of hydrated electrons coming from argon glow discharge. Meanwhile, the metal ions in solution could be simultaneously reduced to MNPs (Au, Pt, Ag and Pd) by these high-energy electrons.…”

“…Generally, peptide serve as mineral source supplier and a multifunctional template with crystal phase control ability. 19 In our group, related works have already been carried out. At the beginning, Wang et al designed specific peptide sequence with two functional motifs.…”

Amyloid‐Mediated Fabrication of Organic–Inorganic Hybrid Materials and Their Biomedical Applications

“…Moreover, mineralization of calcium carbonate or silica has been carried out using the amyloid nanofibril as template as well. [87,88]…”

Regulated Drug Release Abilities of Calcium Carbonate–Gelatin Hybrid Nanocarriers Fabricated via a Self‐Organizational Process

“…However, this involves many steps in the process of their fabrication. With this in mind, in our previous study, we focused on biomineralization, which involves the fabrication of functional materials via a single process . Biomineralization controls the morphology, crystal phase, and functionality of mineralized inorganic matters (e.g., calcium carbonate, calcium phosphate, and silica) via the nanostructure of the organic template surface (e.g., protein or polysaccharide) .…”

“…With this in mind, in our previous study,w ef ocused on biomineralization, which involves the fabrication of functional materials via as ingle process. [13][14][15][16][17][18][19][20][21] Biomineralization controlst he morphology,c rystal phase, and functionality of mineralized inorganic matters( e.g.,c alcium carbonate, calcium phosphate, and silica) via the nanostructure of the organic template surface (e.g.,p rotein or polysaccharide). [22] Collagen is at ypical organic template that has gained interesti ns everal fields of research asafunctional material with aw ide variety of functions.…”