Regulated Drug Release Abilities of Calcium Carbonate–Gelatin Hybrid Nanocarriers Fabricated via a Self‐Organizational Process

Murai, Kazuki; Kurumisawa, Kazuya; Nomura, Yasuya; Matsumoto, Mutsuyoshi

doi:10.1002/cmdc.201700358

Cited by 8 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The calculation results showed that the loading of Exendin4 in PEGDA/HA-4x gel was about 4.17 times and 7.53 times the loading of PEGDA gel in 5 μg/mL and 10 μg/mL Exendin4 solutions respectively, indicating that the introduction of inorganic particles in mineralization process effectively improved the drug loading ability of this kind of biomaterial. 32,33 Then, due to the limited loading capacity of pure hydrogel, the PEGDA gel could only be loaded with limited content of Exendin4, which illustrated the drug loading ability of PEGDA/HA-4x gel in the realistic experimental conditions. From the release curve, the release characteristics of gel immersed in high concentration Exendin4 solution were not significantly different from that of low concentration, while the release of Exendin4 from PEGDA/HA-4x gel had a more stable and longer release cycle, indicating that its potential bone induction ability was better.…”

Section: Discussionmentioning

confidence: 97%

PEGDA/HA mineralized hydrogel loaded with Exendin4 promotes bone regeneration in rat models with bone defects by inducing osteogenesis

Liu

Jing

et al. 2021

J Biomater Appl

View full text Add to dashboard Cite

Scaffolds with osteogenic differentiation function play an important role in the healing process of bone defects. Here, we designed a high strength Poly(ethyleneglycol) diacrylate/Hydroxyapatite (PEGDA/HA) mineralized hydrogel loaded with Exendin4 for inducing osteogenic differentiation. In this study, PEGDA hydrogel was prepared by photo initiating method. PEGDA/HA mineralized hydrogel was prepared by in-situ precipitation method, and Exendin4 was loaded by gel adsorption. The effects of different calcium and phosphorus concentrations on the strength and Exendin4 release of PEGDA/HA hydrogels were investigated. Rat models of bone defect were made and randomly divided into 5 groups. The experimental group was implanted with PEGDA hydrogel, Exendin4-PEGDA hydrogel, PEGDA/HA mineralized hydrogel, Exendin4-PEGDA/HA mineralized hydrogel, and no materials were implanted in the blank control group. Computed tomography (CT) and histology were observed 4 and 8 weeks after operation. Our results revealed that the PEGDA/HA mineralized hydrogel had porous structure, high mechanical strength and good biocompatibility. In vitro release test showed that the mineralized hydrogel exhibited good sustained release profile within 20 d. The animal experiments showed that the mineralized hydrogel accelerated the formation of new bone after 4 and 8 weeks, and formed a seamless union on the defected bone area after 8 weeks. In conclusions, The Exendin4-PEGDA/HA mineralized hydrogel can effectively repair bone defects in rats, and it is expected to be used as a biomaterial for human bone tissue repair.

show abstract

Section: Discussionmentioning

confidence: 97%

PEGDA/HA mineralized hydrogel loaded with Exendin4 promotes bone regeneration in rat models with bone defects by inducing osteogenesis

Liu

Jing

et al. 2021

J Biomater Appl

View full text Add to dashboard Cite

show abstract

“…Murai et al used biomimetic mineralization to create a CaCO 3 -gelatin hybrid nanocarrier. Gelatin is used as an organic scaffold to facilitate the loading of anionic drugs and to control the morphology of CaCO 3 minerals (Murai et al, 2017). Drug release from the nanocarrier was inhibited at neutral pH and triggered in acidic microenvironment of the tumor.…”

Section: Cell Deliverymentioning

confidence: 99%

Novel nanomaterial–organism hybrids with biomedical potential

Cui

Wang

et al. 2021

WIREs Nanomed Nanobiotechnol

View full text Add to dashboard Cite

Instinctive hierarchically biomineralized structures of various organisms, such as eggs, algae, and magnetotactic bacteria, afford extra protection and distinct performance, which endow fragile organisms with a tenacious ability to adapt and survive. However, spontaneous formation of hybrid materials is difficult for most organisms in nature. Rapid development of chemistry and materials science successfully obtained the combinations of organisms with nanomaterials by biomimetic mineralization thus demonstrating the reproduction of the structures and functions and generation of novel functions that organisms do not possess. The rational design of biomaterial–organism hybridization can control biological recognition, interactions, and metabolism of the organisms. Thus, nanomaterial–organism hybrids represent a next generation of organism engineering with great potential biomedical applications. This review summarizes recent advances in material‐directed organism engineering and is mainly focused on biomimetic mineralization technologies and their outstanding biomedical applications. Three representative types of biomimetic mineralization are systematically introduced, including external mineralization, internal mineralization, and genetic engineering mineralization. The methods involving hybridization of nanomaterials and organisms based on biomimetic mineralization strategies are described. These strategies resulted in applications of various nanomaterial–organism hybrids with multiplex functions in cell engineering, cancer treatment, and vaccine improvement. Unlike classical biological approaches, this material‐based bioregulation is universal, effective, and inexpensive. In particular, instead of traditional medical solutions, the integration of nanomaterials and organisms may exploit novel strategies to solve current biomedical problems. This article is categorized under: Implantable Materials and Surgical Technologies > Nanomaterials and Implants Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease

show abstract

“…DDS is a system that delivers the drugs “in a minimum amount”, “at the right time”, and “to the right place”. Polymer micelles [ 5 , 6 ], dendrimers [ 7 , 8 , 9 ], hydrogels [ 10 , 11 , 12 , 13 ], and mesoporous nanoparticles [ 14 , 15 , 16 ] have been reported as DDS carriers. In addition, vesicles have been used for DDS carriers.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of CaCO3-Coated Vesicles by Biomineralization and Their Application as Carriers of Drug Delivery Systems

Miyamaru

Koide

Kato

et al. 2022

IJMS

View full text Add to dashboard Cite

We fabricated CaCO3-coated vesicles as drug carriers that release their cargo under a weakly acidic condition. We designed and synthesized a peptide lipid containing the Val-His-Val-Glu-Val-Ser sequence as the hydrophilic part, and with two palmitoyl groups at the N-terminal as the anchor groups of the lipid bilayer membrane. Vesicles embedded with the peptide lipids were prepared. The CaCO3 coating of the vesicle surface was performed by the mineralization induced by the embedded peptide lipid. The peptide lipid produced the mineral source, CO32−, for CaCO3 mineralization through the hydrolysis of urea. We investigated the structure of the obtained CaCO3-coated vesicles using transmission electron microscopy (TEM). The vesicles retained the spherical shapes, even in vacuo. Furthermore, the vesicles had inner spaces that acted as the drug cargo, as observed by the TEM tomographic analysis. The thickness of the CaCO3 shell was estimated as ca. 20 nm. CaCO3-coated vesicles containing hydrophobic or hydrophilic drugs were prepared, and the drug release properties were examined under various pH conditions. The mineralized CaCO3 shell of the vesicle surface was dissolved under a weakly acidic condition, pH 6.0, such as in the neighborhood of cancer tissues. The degradation of the CaCO3 shell induced an effective release of the drugs. Such behavior suggests potential of the CaCO3-coated vesicles as carriers for cancer therapies.

show abstract

Regulated Drug Release Abilities of Calcium Carbonate–Gelatin Hybrid Nanocarriers Fabricated via a Self‐Organizational Process

Cited by 8 publications

References 29 publications

PEGDA/HA mineralized hydrogel loaded with Exendin4 promotes bone regeneration in rat models with bone defects by inducing osteogenesis

PEGDA/HA mineralized hydrogel loaded with Exendin4 promotes bone regeneration in rat models with bone defects by inducing osteogenesis

Novel nanomaterial–organism hybrids with biomedical potential

Fabrication of CaCO3-Coated Vesicles by Biomineralization and Their Application as Carriers of Drug Delivery Systems

Contact Info

Product

Resources

About