Mineral crystals are smaller and less oriented in osteogenesis imperfecta mouse model, as revealed by small-angle X-ray scattering

Pratzl, P.; Paris, Oskar; Landis, W J; Klaushofer, Klaus

doi:10.1016/8756-3282(95)90481-6

Cited by 1 publication

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“…Marked alterations in the substructure and organization of the collagen fibrils in oim tail tendon were shown, including kinking and lack of banding. Although these alterations are expected in oim from bulk analyses, which take average measurements, ,, we were able to show, using high resolution TEM, that some regions of the oim tissues were still able to form collagen fibrils with the characteristic periodic banding pattern of collagen type I fibrils, identical to that in WT bone and tendon. The mature oim collagen fibrils, however, had smaller diameters than fibrils seen in the WT tendon, and crystals within oim fibrils were less organized.…”

Section: Discussionmentioning

confidence: 77%

“…Previous studies have demonstrated a strong relationship between the overall strength of the material and the orientation and morphology of the mineral crystals. In OI tissues, the long axes of crystals exhibit a deviation from their alignment parallel to collagen fibrils, , and a great variation in the shape and size of the apatite crystals is observed . The mineral crystals are smaller, more randomly aligned and distributed in OI bone compared to that in healthy bone. ,− There also have been reports about the formation of large bulky crystals and the aggregation of small crystals seen in oim . ,, These reports are not contradictory.…”

Section: Introductionmentioning

confidence: 99%

“…In OI tissues, the long axes of crystals exhibit a deviation from their alignment parallel to collagen fibrils, , and a great variation in the shape and size of the apatite crystals is observed . The mineral crystals are smaller, more randomly aligned and distributed in OI bone compared to that in healthy bone. ,− There also have been reports about the formation of large bulky crystals and the aggregation of small crystals seen in oim . ,, These reports are not contradictory. The presence and organization of small crystals was assessed mainly by small-angle X-ray scattering (SAXS), which is not optimal for detection of structures larger than 50 nm .…”

Section: Introductionmentioning

confidence: 99%

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Electron Microscopy Reveals Structural and Chemical Changes at the Nanometer Scale in the Osteogenesis Imperfecta Murine Pathology

Kłosowski

Carzaniga

Abellán

et al. 2016

ACS Biomater. Sci. Eng.

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Alternations of collagen and mineral at the molecular level may have a significant impact on the strength and toughness of bone. In this study, scanning transmission electron microscopy (STEM) and electron energy-loss spectroscopy (EELS) were employed to study structural and compositional changes in bone pathology at nanometer spatial resolution. Tail tendon and femoral bone of osteogenesis imperfecta murine (oimbrittle bone disease) and wild type (WT) mice were compared to reveal defects in the architecture and chemistry of the collagen and collagen-mineral composite in the oim tissue at the molecular level. There were marked differences in the sub-structure and organization of the collagen fibrils in oim tail tendon; some regions have clear fibril banding and organization, while in other regions fibrils are disorganized. Malformed collagen fibrils were loosely packed, often bent and devoid of banding pattern. In bone, differences were detected in the chemical composition of mineral in oim and WT. While mineral present in WT and oim bone exhibited the major characteristics of apatite, examination in EELS of the fine structure of the carbon K ionisation edge revealed a significant variation in the presence of carbonate in different regions of bone. Variations have been also observed in the fine structure and peak intensities of the nitrogen K-edge.These alterations are suggestive of differences in the maturation of collagen nucleation sites or crosslinks. Future studies will aim to establish the scale and impact of the modifications observed in oim tissues. The compositional and structural alterations at the molecular level cause deficiencies at larger length scales. Understanding the effect of molecular alterations to pathologic bone is critical to the design of effective therapeutics.

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Section: Discussionmentioning

confidence: 77%