Milt Properties of Four Flatfish Species and Fine Structure of Their Cryopreserved Spermatozoa

Jeong, Chang Yun; Jin, Chang Young

doi:10.5657/fas.2002.5.2.087

Cited by 8 publications

(9 citation statements)

References 33 publications

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“…Similarly, the osmolality value of Striped jewfish seminal plasma was lower than in Grey mullet ( Mugil cephalus) ; 370.0 mOsm/kg (Chang et al., ) and in Black porgy ( Acanthopagrus schlegeli ); 382.0 mOsm/kg (Chang, Lim, & Kho, ). The pH value of seminal plasma in the present study was similar to values obtained in other studies (Chang & Chang, ; Chang et al., ; Dziewulska et al., ), and higher than what was reported by Bozkurt, Secer, Bukan, Akcay and Tekin () and lower than values in a report by Chang et al. (), Lahnsteiner et al.…”

Section: Discussionsupporting

confidence: 90%

“…The spermatocrit (79 ± 3%) of striped jewfish was higher than reported in other species (Aas, Refstie, & Gjerde, ; Cruz‐Casallas, Lombo‐Rodríguez, & Velasco‐Santamaría, ; Dziewulska, Rzemieniecki, & Domagala, ; Le, Lim, Min, Kim, & Chang, ; Lim et al., , ; Suquet, Omnes, Normant, & Fauvel, ) and lower than that of Grey mullet Mugil cephalus (Chang, Choi, Lim, & Kho, ) and other flat fish species (Chang & Chang, ). The spermatocrit of semen also varied between conspecific males, species and across the reproductive season (Rakitin, Ferguson, & Trippel, ; Rurangwa et al., ).…”

Section: Discussionmentioning

confidence: 54%

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Properties and activities of blood- or seawater-contaminated wild-caught Striped Jewfish (Stereolepis doederleini) sperm

Chang

Arukwe

2017

Aquac Res

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Understanding the effects of environmental factors in sperm qualities will be helpful in the development of optimal artificial reproduction methods and contributes towards the knowledge base of better short-and long-term fish semen preservation conditionsThe objectives of this study were to determine properties and activities of wild-caught striped jewfish Stereolepis doederleini sperm contaminated with blood or seawater and compare them with data reported in the literature on other freshwater and marine fish species, for effective short-and long-term storage of fish semen. Overall, we observed that the sodium, chloride, glucose, total protein concentrations of normal sperm were not significantly different from blood-or seawater-contaminated sperm. The salinity and osmolality concentration of sperm contaminated with blood were lower than sperm contaminated with seawater and were not significantly different from normal sperm. In addition, the spermatozoa motility (SM) and duration of spermatozoa motility (DSM) in blood-contaminated sperm were higher than seawater-contaminated sperm and also not significantly different from normal sperm. The best condition for SM and DSM in normal sperm was dilution rate of 1:50. Sperm was immotile in distilled water, and cationic factors were shown to stimulate the initiation of spermatozoa activation.The maximum SM and DSM were observed in solution containing 0.4 M NaCl, 0.6 M KCl, 0.6 M CaCl 2 and 0.4 M MgCl 2 . This study provides some basic and important knowledge about striped jewfish sperm sensitivity to a cationic condition. In this regard, Na + is the major inhibitory factor of spermatozoa motility in this fish species.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 54%

Properties and activities of blood- or seawater-contaminated wild-caught Striped Jewfish (Stereolepis doederleini) sperm

Chang

Arukwe

2017

Aquac Res

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“…The changes might adversely affect the function of mitochondria and tails, thus reducing the sperm flagellate movement. Similar changes have also been reported in post-thawed sperm of the Atlantic croaker Micropogonias undulatus (Gwo and Arnold 1992), the founder Paralichthys olivaceus (Zhang et al 2003), the grayling Thymallus thymallus (Lahnsteiner et al 1992); striped bass Morone saxatilis (He and Wood III 2004); ocean pout Macrozoarces americanus (Yao et al 2000); black porgy Acanthopagrus schlegeli (Lim and Chang 1998;); four flatfish species (Chang 2001;Chang and Chang 2002); pointhead flounder Cleisthenes pinetorum herzensteini (Kho and Kang 2003) and filefish (Le 2008). Sperm was damaged after freezing due to the components of extender, cryoprotectant concentration or type, freezing rate, thawing temperature, etc.…”

Section: Discussionmentioning

confidence: 56%

Semen cryopreservation of yellow croaker Larimichthys polyactis

Lim

Min

et al. 2011

Rev Fish Biol Fisheries

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The effects of various extenders and cryoprotectants on movable spermatozoa ratio (MSR), spermatozoa velocity (SV) and duration of spermatozoa motility (DSM) of post-thawed spermatozoa were examined. The MSR, SV and DSM of post-thawed sperm in artificial seminal plasma (ASP) extender were higher than those in marine fish Ringer's solution (MFRS) extender (P \ 0.01) and was not significantly different from that of fresh sperm. No significant differences were observed in the motility parameters between fresh spermatozoa and frozen-thawed spermatozoa cryopreserved with ASP extender supplement 10% EG (ethylene glycol) cryoprotectant. Using the above method, yellow croaker semen was cryopreserved with extender ASP and 10% EG. As a result, at the spermatozoa/egg ratio of 100,000:1, the fertilization rate and hatching rate of the frozen-thawed spermatozoa cryopreserved for 1 week or 1 year in liquid nitrogen (45.7 ± 3.2% and 27.2 ± 5.0% or 37.5 ± 4.4% and 27.2 ± 5.0%) were similar to that of fresh spermatozoa (51.0 ± 3.1% and 36.7 ± 2.2%).There was a small alternation of shape in cryopreserved spermatozoa compared with fresh spermatozoa. In conclusion, the optimal conditions for yellow croaker semen cryopreservation are ASP extender supplement 10% EG cryoprotectant. This is the first report on semen cryopreservation of yellow croaker Larimichthys polyactis.

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“…Paired lateral fins along the flagellum are observed in M. barbatus, but a single-side fin was reported in B. boops and D. sargus (Lahnsteiner and Patzner 1998). In the present study, no fin was observed in sperm of Atlantic halibut, similar to those of Pagrus major, R. sabra, Parupeneus spilurus, S. fuscescens, and T. mediterraneus, but the axoneme was surrounded by a wide layer of vacuolated cytoplasm similar to those of Paralichthys olivaceus, Platichthys stellatus, Pseudopleuronectes yokohamae, and Pseudopleuronectes herzensteini (Gwo et al 2004a(Gwo et al , 2004bChang and Chang 2002). In the present study, we also observed vacuolated cytoplasm around the nucleus and in the midpiece.…”

Section: Discussionsupporting

confidence: 37%

“…Previous studies demonstrated interspecies and family differences for head shape, number of mitochondria, location and positions of proximal and distal centrioles, presence of a fin structure, and dimensions of head, midpiece, and flagellum. For example, spermatozoa of starry flounder (Platichthys stellatus (Pallas, 1788)), marbled flounder (Pseudopleuronectes yokohamae (Günther, 1877)), little mouth flounder (Pseudopleuronectes herzensteini (Jordan and Snyder, 1901)), Mediterranean horse mackerel (Trachurus mediterraneus (Steindachner, 1868)), boga (Boops boops (L., 1758)), and white seabream (Diplodus sargus (L., 1758)) have a head of ovoid or ellipsoidal shape, but spermatozoa of dusky spinefoot (Siganus fuscescens (Houttuyn, 1782)), Atlantic halibut (present study), olive flounder (Paralichthys olivaceus (Temminck and Schlegel, 1846)), red seabream (Pagrus major (Temminck and Schlegel, 1843)), and goldline seabream (Rhabdosargus sarba (Forsskål, 1775)) have a spherical-shaped head (Lahnsteiner and Patzner 1998;Chang and Chang 2002;Gwo et al 2004aGwo et al , 2004b. Spermatozoa of bluntsnouted mullet (Mullus barbatus L., 1758) and Japanese goatfish (Parupeneus spilurus) have long heads that are flattened on one side (Lahnsteiner and Patzner 1998;Gwo et al 2004b).…”

Section: Discussionmentioning

confidence: 96%

Sperm morphology, ATP content, and analysis of motility in Atlantic halibut (Hippoglossus hippoglossus)

et al. 2011

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Spermatozoon of Atlantic halibut (Hippoglossus hippoglossus (L., 1758)) is uniflagellated, lacks an acrosome, and is differentiated into a head, midpiece, and flagellum. There are two to five mitochondria in the midpiece, as well as proximal and distal centrioles. The flagellum consisted of 9 + 2 microtubules surrounded by plasma membrane, which is extended at the proximal part of the flagellum owing to the presence of vacuoles. After sperm activation in seawater, sperm motility and velocity decreased from 98.4% ± 3.4% and 170.3 ± 8.9 mmÁs -1 at 15 s after sperm activation to 4.8% ± 4.7% and 9.2 ± 8.9 mmÁs -1 at 120 s after sperm activation, respectively. ATP content (nmolÁL -1 ATP per 10 8 spermatozoa) significantly decreased at 60 s after sperm activation (5.9 ± 1.5) compared with at 0 and 30 s after sperm activation (14.9 ± 1.5 and 14.5 ± 1.5, respectively). Beating waves propagated along the full length of the flagellum after sperm activation, whereas waves were restricted to the proximal section during the latter motility period. Wave amplitude significantly decreased at 45 s after sperm activation, but wavelength did not differ. The present study showed associations among sperm morphology, ATP content, flagellar wave parameters, and sperm velocity, which could be used in comparative spermatology.Résumé : Le spermatozoïde du flétan atlantique (Hippoglossus hippoglossus (L., 1758)) est uniflagellé, sans acrosome et différencié en tête, corps médian et flagelle. Il y a deux à cinq mitochondries dans le corps médian, ainsi que des centrioles proximaux et distaux. Le flagelle consiste en 9 + 2 microtubules entourés d'une membrane plasmatique qui se prolonge dans la partie proximale du flagelle à cause de la présence de vacuoles. Après l'activation des spermatozoïdes dans l'eau de mer, la motilité et la vitesse des spermatozoïdes diminuent respectivement de 98,4 % ± 3,4 % et de 170,3 ± 8,9 mmÁs -1 à 15 s après l'activation à 4,8 % ± 4,7 % et 9,2 ± 8,9 mmÁs -1 à 120 s après l'activation. Le contenu en ATP (nmolÁL -1 ATP par 10 8 spermatozoïdes) diminue significativement 60 s après l'activation (5,9 ± 1,5) par comparaison à 0 et 30 s après l'activation (respectivement 14,9 ± 1,5 et 14,5 ± 1,5). Les ondulations de battement se propagent sur toute la longueur du flagelle après l'activation, mais sont restreintes à la partie proximale durant la période de motilité subséquente. L'amplitude des ondulations diminue significativement à 45 s après l'activation, mais leur longueur d'onde ne change pas. Notre étude met en lumière des associations entre la morphologie des spermatozoïdes, leur contenu en ATP, les variables ondulatoires des flagelles et la vitesse des spermatozoïdes qui pourraient servir en spermatologie comparée.[Traduit par la Rédaction]

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Milt Properties of Four Flatfish Species and Fine Structure of Their Cryopreserved Spermatozoa

Cited by 8 publications

References 33 publications

Properties and activities of blood- or seawater-contaminated wild-caught Striped Jewfish (Stereolepis doederleini) sperm

Properties and activities of blood- or seawater-contaminated wild-caught Striped Jewfish (Stereolepis doederleini) sperm

Semen cryopreservation of yellow croaker Larimichthys polyactis

Sperm morphology, ATP content, and analysis of motility in Atlantic halibut (Hippoglossus hippoglossus)

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