Semen cryopreservation of yellow croaker Larimichthys polyactis

Abstract

The effects of various extenders and cryoprotectants on movable spermatozoa ratio (MSR), spermatozoa velocity (SV) and duration of spermatozoa motility (DSM) of post-thawed spermatozoa were examined. The MSR, SV and DSM of post-thawed sperm in artificial seminal plasma (ASP) extender were higher than those in marine fish Ringer's solution (MFRS) extender (P \ 0.01) and was not significantly different from that of fresh sperm. No significant differences were observed in the motility parameters between fresh spermatozoa and frozen-thawed spermatozoa cryopreserved with ASP extender supplement 10% EG (ethylene glycol) cryoprotectant. Using the above method, yellow croaker semen was cryopreserved with extender ASP and 10% EG. As a result, at the spermatozoa/egg ratio of 100,000:1, the fertilization rate and hatching rate of the frozen-thawed spermatozoa cryopreserved for 1 week or 1 year in liquid nitrogen (45.7 ± 3.2% and 27.2 ± 5.0% or 37.5 ± 4.4% and 27.2 ± 5.0%) were similar to that of fresh spermatozoa (51.0 ± 3.1% and 36.7 ± 2.2%).There was a small alternation of shape in cryopreserved spermatozoa compared with fresh spermatozoa. In conclusion, the optimal conditions for yellow croaker semen cryopreservation are ASP extender supplement 10% EG cryoprotectant. This is the first report on semen cryopreservation of yellow croaker Larimichthys polyactis.

Sperm Characteristics of Wild European Flounder (Platichthys flesus luscus)

“…The relationship between semen volume and fish weight was similar to the relationships in yellow croaker (r = 0.975, p<0.05; Le et al, 2011) and cultured European flounder (r = 0.990, p<0.01; Aydın et al, 2011). In contrast, the relationship between semen volume and fish weight was insignificant in scaly carp (r = 0.2580, p>0.05; Bozkurt, 2006) and brown trout (r = 0.4310, p>0.05; Bozkurt et al, 2006).…”

Evaluation of different extenders for the cold storage of meagre (Argyrosomus regius ) semen

“…Our results indicate that both 0.9% NaCl and 0.9% NaCl with glucose and glycine are good options. Different options should be tested, such as the addition of nutrients, antioxidants (Ubilla & Valdebenito, ) or antibacterial substances (Le, Lim, Min, Park, & Chang, ). Nonetheless, the extenders tested in this study can easily be incorporated in a hatchery environment due to their simplicity.…”

Seasonal Changes in the Milt Quality of Waigieu Seaperch, Psammoperca waigiensis: Implications for Artificial Propagation

“…The milt volume of Waigieu seaperch was similar to that of other marine fishes (e.g., turbot, Psetta maxima [Suquet et al ]; yellow tail flounder, Limanda ferruginea [Clearwater and Crim ]; filefish, Thamnaconus modestus [Le et al ]; Brazilian flounder, Paralichthys orbignyanus [Lanes et al ]; yellow croaker, Larimichthys polyactis [Le et al ]; and rabbit fish, Siganus guttatus [Vo et al ]), which produced <1.5 mL. The milt volume in these species was also less than that in other marine fish species such as Asian seabass, Lates calcarifer (Hilomen‐Garcia et al ); European seabass, Dicentrarchus labrax (Öğretmen et al ); black porgy, Acanthopagrus schlegeli (Chang et al ); and tiger grouper, Epinephelus fuscoguttatus (Le et al ), which reached more than 1.5 mL.…”

“…The sperm concentration in milt for Waigieu seaperch was higher than that of yellow croaker (Le et al ) and lower than that of turbot (Suquet et al ). Understanding how the sperm concentration in milt changes throughout the spawning season is necessary for the determination of optimal sperm : egg ratios essential to maximize fertilization success (Butts et al ; Johnson et al ).…”

“…The head of sperm (<2 μm in width) in Waigieu seaperch was bigger than that of yellow croaker (<1.6 μm in width) (Le et al ) and filefish (<1.4 μm in width) (Le et al ), but smaller than that in other teleost fishes (from 2 to 4 μm in width) (Jamieson ). Additionally, the sperm has the kidney shape that is similar to Mediterranean rainbow wrasse, Coris julis (Jamieson ), and yellow croaker (Le et al ), but is different from other teleost fish such as ball‐shaped in northern pike, Esox lucius; ovoid‐shaped in cardinal fish, Apgon imberbis ; banana‐shaped in Atlantic eel, Anguilla anguilla (Jamieson ); horse‐shoe‐shaped in filefish (Le et al ); and elongated in sturgeon (Golpour et al ). Alavi et al () reported the changes in sperm morphological and ultrastructural parameters in the freshwater species Barbus barbus during the spawning season.…”