Millisecond lifetime imaging with a europium complex using a commercial confocal microscope under one or two-photon excitation

Grichine, Alexeï; Haefelé, Alexandre; Pascal, Simon; Duperray, Alain; Michel, Richard; Andraud, Chantal; Maury, Olivier

doi:10.1039/c4sc00473f

Cited by 86 publications

(101 citation statements)

References 62 publications

(67 reference statements)

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“…Very recently, Grichine et al demonstrated how live cell lifetime microscopy in the μs-ms range can be conducted, in the time domain, on a commercial microscope without further modification, under both one-and two-photon excitation [94]. The authors made use of a Eu 3+ complex (τ ¼ 1.06 ms in water) to demonstrate the capability of the technique, which they referred to as Temporal Sampling Lifetime Imaging Microscopy (TSLIM), a complementary technique to Pinhole Scanning Lifetime Imaging Microscopy (PSLIM) typically used for long lived time-gated Reprinted from [87], with kind permission from Springer Science + Business Media.…”

Section: Confocal Lifetime Imaging For Millisecond Emittersmentioning

confidence: 98%

Time-Resolved Emission Imaging Microscopy Using Phosphorescent Metal Complexes: Taking FLIM and PLIM to New Lengths

Baggaley

Weinstein

Williams

2014

Luminescent and Photoactive Transition Metal Complexes as Biomolecular Probes and Cellular Reagents

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Luminescent metal complexes are increasingly being investigated as emissive probes and sensors for cell imaging using what is traditionally termed fluorescence microscopy. The nature of the emission in the case of second-and third-row metal complexes is phosphorescence rather than fluorescence, as it emanates from triplet rather than singlet excited states, but the usual terminology overlooks the distinction between the quantum mechanical origins of the processes. In steady-state imaging, such metal complexes may be alternatives to widely used fluorescent organic molecules, used in exactly the same way but offering advantages such as ease of synthesis and colour tuning. However, there is a striking difference compared to fluorescent organic molecules, namely the much longer lifetime of phosphorescence compared to fluorescence. Phosphorescence lifetimes of metal complexes are typically around a microsecond compared to the nanosecond values found for fluorescence of organic molecules. In this contribution, we will discuss how these long lifetimes can be put to practical use. Applications such as time-gated imaging allow discrimination from background fluorescence in cells and tissues, while increased sensitivity to quenchers provides a means of designing more responsive probes, for example, for oxygen. We also describe how the technique of fluorescence lifetime imaging microscopy (FLIM) -which provides images based on lifetimes at different points in the image -can be extended from the usual nanosecond range to microseconds. Key developments in instrumentation as well as the properties of complexes suitable for the purpose are discussed, including the use of two-photon excitation methods. A number of different research groups have made pioneering contributions to the instrumental set-ups, but the E. Baggaley and J.A. Weinstein Department of Chemistry, University of Sheffield, Sheffield S3 7HF, UK e-mail: liz.baggaley@sheffield.ac.uk; julia.weinstein@sheffield.ac.uk J.A.G. Williams (*) Department of Chemistry, Durham University, Durham DH1 3LE, UK e-mail: j.a.g.williams@durham.ac.uk terminology and acronyms have not developed in a systematic way. We review the distinction between time-gating (to eliminate background emission) and true timeresolved imaging (whereby decay kinetics at each point in an image are monitored). For instance, terms such as PLIM (phosphorescence lifetime imaging microscopy) and TRLM (time-resolved luminescence microscopy) refer essentially to the same technique, whilst TREM (time-resolved emission imaging microscopy) embraces these long timescale methods as well as the more well-established technique of FLIM.Keywords Cell imaging Á Fluorescence Á Fluorescence lifetime imaging microscopy Á Imaging Á Iridium Á Metal complex Á Microscopy Á Oxygen sensing Á Phosphorescence Á Phosphorescence lifetime imaging microscopy Á Platinum Á Ruthenium Á Time-resolved emission imaging microscopy Á Time-resolved fluorescence Á Time-resolved luminescence microscopy Á Tissue imaging

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Section: Confocal Lifetime Imaging For Millisecond Emittersmentioning

confidence: 98%

Time-Resolved Emission Imaging Microscopy Using Phosphorescent Metal Complexes: Taking FLIM and PLIM to New Lengths

Baggaley

Weinstein

Williams

2014

Luminescent and Photoactive Transition Metal Complexes as Biomolecular Probes and Cellular Reagents

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“…[20] In the molecular-magnetism field, the luminescence was recently exploited to provide a high level of comprehension of the magnetic properties. [21][22][23][24][25][26][27][28][29] Clearly, the coexistence of magnetic and optical properties enriches the family of multiproperty magnetic objects, and luminescent SMMs have emerged. A certain number of prerequisites are needed to favour the observation of both SMM behaviour and lanthanide luminescence.…”

Section: Introductionmentioning

confidence: 99%

Luminescence and Single‐Molecule‐Magnet Behaviour in Lanthanide Coordination Complexes Involving Benzothiazole‐Based Tetrathiafulvalene Ligands

et al. 2017

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“…This technique is the best suited for the images formed by lanthanide chelates and metal-ligand complexes, possessing long lifetimes (Grichine et al 2014 ). The time-resolved imaging allows more fl exibility, and a combination of intensity and lifetime imaging allows improving the application of FRET (Hanley et al 2002 ), which is a key approach to elucidating the proximity relations and dynamics inside the cell.…”

Section: Time-resolved and Time-gated Imagingmentioning

confidence: 99%

Sensing Inside the Living Cells

Demchenko

2015

Introduction to Fluorescence Sensing

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Millisecond lifetime imaging with a europium complex using a commercial confocal microscope under one or two-photon excitation

Abstract: International audienc

Cited by 86 publications

References 62 publications

Time-Resolved Emission Imaging Microscopy Using Phosphorescent Metal Complexes: Taking FLIM and PLIM to New Lengths

Time-Resolved Emission Imaging Microscopy Using Phosphorescent Metal Complexes: Taking FLIM and PLIM to New Lengths

Luminescence and Single‐Molecule‐Magnet Behaviour in Lanthanide Coordination Complexes Involving Benzothiazole‐Based Tetrathiafulvalene Ligands

Sensing Inside the Living Cells

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