2013
DOI: 10.1021/jf402221m
|View full text |Cite
|
Sign up to set email alerts
|

Milk Whey Protein Modification by Coffee-Specific Phenolics: Effect on Structural and Functional Properties

Abstract: A suitable vehicle for integration of bioactive plant constituents is proposed. It involves modification of proteins using phenolics and applying these for protection of labile constituents. It dissects the noncovalent and covalent interactions of β-lactoglobulin with coffee-specific phenolics. Alkaline and polyphenol oxidase modulated covalent reactions were compared. Tryptic digestion combined with MALDI-TOF-MS provided tentative allocation of the modification type and site in the protein, and an in silico m… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

20
94
1

Year Published

2015
2015
2024
2024

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 124 publications
(115 citation statements)
references
References 33 publications
20
94
1
Order By: Relevance
“…Higher DH values reflect the more ordered structure as was evident in the XRD and CD results. Similar results were reported by Ali et al (2013) on whey protein upon modification with coffee specific phenolics.…”
Section: Differential Scanning Calorimetrysupporting
confidence: 90%
See 3 more Smart Citations
“…Higher DH values reflect the more ordered structure as was evident in the XRD and CD results. Similar results were reported by Ali et al (2013) on whey protein upon modification with coffee specific phenolics.…”
Section: Differential Scanning Calorimetrysupporting
confidence: 90%
“…Similar results were reported by Hasni et al (2011) on casein protein and it was revealed that conformation was changed by phenolic compound with major reduction of a-helix and b-sheet and increase of random coil. Ali et al (2013) used coffee specific phenolic to modify the whey proteins. They reported that there was a reduction in structured elements and increase in unordered structure upon incubation of protein with coffee specific phenolic compounds.…”
Section: Results and Discussion Circular Dichroism (Cd) Spectroscopymentioning
confidence: 99%
See 2 more Smart Citations
“…If two 60 nucleophiles from different protein molecules react with the same 61 phenolic compound (4), a phenol-mediated protein cross-link is 62 created (Scheme 1) [5][6][7]. 63 The formation of protein-phenol adducts or cross-links modi-64 fies the physicochemical properties and functionality of the protein 65 [8][9][10][11][12], and such adducts may generate hazardous toxicological 66 effects in vivo, including cytotoxicity, immunotoxicity, and car-67 cinogenesis [13]. For example, phenol adducts formed with cyto-68 chrome c have been found to interfere with its proapoptotic 69 function [3], and as reviewed by Bolton and coworkers [13], it 70 has been established that estrogen is metabolized into catechols 71 and quinones, which may be responsible for the development of 72 breast cancer in women.…”
mentioning
confidence: 99%