Milk Fat Globule Membrane Material

Effects of mono‐ and diglycerides of fatty acids on the milk fat globule membrane after heat treatment

“…Heating and isolation of MFGM material from the samples CH, MD1, MD2 and MD3 were kept at 65°C for 30 min in a water bath. The isolation procedure was divided into four stages: fat separation, cream washing, release of the MFGM from the globules and the collection of the MFGM material (Le et al 2014) as shown in Figure 1. The heated samples were centrifuged after cooling at 10 000 g for 20 min (4°C) (H1850R, Cence Company, Beijing, China).…”

“…The MFGM consists of a lipid core and three layers. The major proteins in MFGM include the glycoprotein mucin 1 (MUC1), the redox enzyme xanthine dehydrogenase/oxidase (XDH/ XO), periodic acid Schiff 3 (PAS3), cluster of differentiation 36 (CD 36), periodic acid Schiff 6/7 (PAS6/7), butyrophilin (BTN) and adipophilin (ADPH; Le et al 2014;Holzmuller et al 2016). Milk fat globule membrane proteins are located in different parts of the membrane.…”

“…Due to its affinity for triglycerides, adipophilin (ADPH) is located in the inner polar lipid monolayer of the membrane. Xanthine dehydrogenase/oxidase (XDH/XD) is located at the outer part of the milk fat globule membrane (Le et al 2014). Correding and Dalgleish (1998) used MFGM material isolated from untreated cream as an emulsifier to form stable soya oil-in-water emulsions.…”

“…phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylinositol (PI)] and sphingolipids [mainly milk sphingomyelin (MSM)]. Glycerophospholipids consist of a glycerol backbone on which two fatty acids (FAs) are esterified (Dewettinck et al 2008;Gallier et al 2012;Le et al 2014).…”

The effects of sodium hexametaphosphate addition on the physicochemical properties and microstructure of the milk fat globule membrane (MFGM) in liquid milk after freezing

“…The isolation procedure was divided into four stages: fat separation, cream washing, release of the MFGM from the globules and the collection of the MFGM material ( Figure 1; Le et al 2014).…”