In this study, the effect of mono-and diglycerides (M-DGs) on milk fat globule membrane properties after heating was studied. The samples with M-DGs exhibited higher numbers of milk fat globule membrane (MFGM) particles (6-7%) compared to the other samples without M-DGs (5.5%). The samples with low concentrations of M-DG were more stable than the samples without M-DG. Sodium dodecyl sulphate polyacrylamide gel electrophoresis results showed that the bonds of MFGM proteins were strongly stained in the samples with M-DGs compared to heated samples without M-DG. M-DGs are promising emulsifiers as they may protect MFGM during heating and therefore improve the stability of MFGM emulsions.