Milk exosomes-mediated miR-31-5p delivery accelerates diabetic wound healing through promoting angiogenesis

Yan, Chengqi; Chen, Jing; Wang, Cheng; Yuan, Meng; Kang, Yu; Wu, Zihan; Li, Wenqing; Zhang, Guolei; Machens, Hans‐Günther; Rinkevich, Yuval; Chen, Zhenbing; Yang, Xiaofan; Xu, Xiang Xi

doi:10.1080/10717544.2021.2023699

Cited by 119 publications

(108 citation statements)

References 98 publications

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“…[49][50][51] Shi et al 52 showed that TGF-β-loaded exosomes could accelerate wound healing both in vitro and in vivo. Yan et al 53 4034 milk-derived exosomes achieved higher cell uptake and was able to resist degradation, and also dramatically improved endothelial cell functions in vitro, together with the promotion of angiogenesis and enhanced diabetic wound healing in vivo, which showed the feasibility of milk-derived exosomes as a scalable, biocompatible, and costeffective delivery system to enhance the bioavailability and efficacy of miRNAs.…”

Section: Discussionmentioning

confidence: 99%

Exosome Derived from Mesenchymal Stem Cells Alleviates Pathological Scars by Inhibiting the Proliferation, Migration and Protein Expression of Fibroblasts via Delivering miR-138-5p to Target SIRT1

Zhao¹,

Zhang²,

Zang³

et al. 2022

IJN

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Introduction The therapies of using exosomes derived from mesenchymal stem cells (MSC-Exo) for wound healing and scar attenuation and micro RNAs (miRNAs) for regulation of genes by translational inhibition and mRNA destabilization obtained great achievements. Silent information regulator 1 (SIRT1) is the silent information, which has an intricate role in many biological processes. However, the effects of SIRT1 and miR-138-5p loaded in MSC-Exo on pathological scars remain unclear. Methods MSC-Exo was isolated and identified by ultracentrifugation, transmission electron microscopy, nanoparticle size measuring instrument and Western blot assays. The relationship between SIRT1 and miR-138-5p was verified by a double-luciferase reporter assay. Cell Counting Kit-8, Τranswell, scratch, and Western blot assays were used to evaluate the proliferation and migration of human skin fibroblasts (HSFs), and the protein expression of SIRT1, NF-κB, α-SMA and TGF-β1 in HSFs, respectively. Flow cytometry was used to assess the apoptosis and cell cycle of HSFs affected by SIRT1. Results Our study demonstrated that miR-138-5p loaded in MSC-Exo could attenuate proliferation, migration and protein expression of HSFs-derived NF-κB, α-SMA, and TGF-β1 by targeting to SIRT1 gene, which confirmed the potential effects of MSC-Exo in alleviating pathological scars by performing as a miRNA’s delivery vehicle. Conclusion Exosomes derived from MSCs acting as a delivery vehicle to deliver miR-138-5p can downregulate SIRT1 to inhibit the growth and protein expression of HSFs and attenuate pathological scars.

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Section: Discussionmentioning

confidence: 99%

Exosome Derived from Mesenchymal Stem Cells Alleviates Pathological Scars by Inhibiting the Proliferation, Migration and Protein Expression of Fibroblasts via Delivering miR-138-5p to Target SIRT1

Zhao¹,

Zhang²,

Zang³

et al. 2022

IJN

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“…For the cluster analysis of references and distribution in a timeline ( Figure 4D ), researchers were particularly interested in EVs for intercellular communication and organizational repair research in 2016, and then researchers seemed to focus more on the microRNA repair mechanism of EVs and the clinical transformation of engineered exosomes. For instance, Yan et al ( 22 ) used milk-derived exosomes as a delivery medium for miR-31-5p, which could effectively promote the healing of diabetic wounds. In terms of the cluster analysis of publications and their distribution in a timeline ( Figures 5B,E ), it is not difficult to find that SC-derived exosomes can improve DM and its complications (especially diabetic wounds and diabetic nephropathy) through various mechanisms such as improving insulin resistance, promoting vascularization, and regulating inflammation, which is a current research hotspot.…”

Section: Discussionmentioning

confidence: 99%

A Bibliometric Analysis of the Hotspots Concerning Stem Cell Extracellular Vesicles for Diabetes in the Last 5 Years

Qiu¹,

Guo

Zhang³

et al. 2022

Front. Public Health

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Background:Diabetes mellitus (DM) is a metabolic disease that endangers human health, and its prevalence is exploding and younger. Stem cell-derived extracellular vesicles (SC-EVs) have a repair function similar to SCs and no risk of tumor formation, which have been widely used in the repair of DM and its complications. We aim to map the hot trends of SC-EVs for the treatment of DM and providing directions for future research.MethodsWe screened all relevant publications on SC-EVs for DM from the Web of Science (Wos) during 2017–2021, and research trends in this field were analyzed by VOSviewer and CiteSpace.ResultsA total of 255 articles related to SC-EVs for DM were screened out according to the search strategy. China (122 publications and 2,759 citations) was the most productive country, followed by the USA (50 publications and 1,167 citations) and Italy (16 publications and 366 citations). The top five institutions with the most publications were located in Italy and China, with Turin University being the most productive. The journals Stem Cell Research and Therapy and International Journal of Molecular Sciences published most of the studies on SC-EVs for DM. ASHOK KUMAR published the majority of articles in this field, while QING LI was the most cited. Cluster analysis indicated that the current research trend is more focused on the repair mechanism and clinical translation of exosomes and their related preparations in promoting DM and its complications.ConclusionIn this study, a comprehensive summary and analysis of the global research trends of SC-EVs used in DM and its complications was performed. In the past 5 years, relevant high-quality publications in this field have increased significantly, and SC-EVs have a good prospect for development in the treatment of DM and its complications.

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“…Extracellular vesicles (EVs) are secreted from almost all cell types ( 1 ), and widely distributed in various body fluids, such as urine ( 2 ), blood ( 3 ), milk ( 4 ), saliva ( 5 ), cerebrospinal fluid ( 6 ), amniotic fluid ( 7 ) and semen ( 8 ), can transmit information between cells and participate in many physiological and pathological processes. It is known that the extraction and isolation of exosomes from different body fluids are mainly achieved by ultracentrifugation, ultrafiltration, sedimentation, density gradient centrifugation, immune-capture, precipitation and commercial reagents( Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

Current perspectives on clinical use of exosomes as novel biomarkers for cancer diagnosis

Chen

Huang

et al. 2022

Front. Oncol.

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Exosomes are a heterogeneous subset of extracellular vesicles (EVs) that biogenesis from endosomes. Besides, exosomes contain a variety of molecular cargoes including proteins, lipids and nucleic acids, which play a key role in the mechanism of exosome formation. Meanwhile, exosomes are involved with physiological and pathological conditions. The molecular profile of exosomes reflects the type and pathophysiological status of the originating cells so could potentially be exploited for diagnostic of cancer. This review aims to describe important molecular cargoes involved in exosome biogenesis. In addition, we highlight exogenous factors, especially autophagy, hypoxia and pharmacology, that regulate the release of exosomes and their corresponding cargoes. Particularly, we also emphasize exosome molecular cargoes as potential biomarkers in liquid biopsy for diagnosis of cancer.

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Milk exosomes-mediated miR-31-5p delivery accelerates diabetic wound healing through promoting angiogenesis

Cited by 119 publications

References 98 publications

Exosome Derived from Mesenchymal Stem Cells Alleviates Pathological Scars by Inhibiting the Proliferation, Migration and Protein Expression of Fibroblasts via Delivering miR-138-5p to Target SIRT1

Exosome Derived from Mesenchymal Stem Cells Alleviates Pathological Scars by Inhibiting the Proliferation, Migration and Protein Expression of Fibroblasts via Delivering miR-138-5p to Target SIRT1

A Bibliometric Analysis of the Hotspots Concerning Stem Cell Extracellular Vesicles for Diabetes in the Last 5 Years

Current perspectives on clinical use of exosomes as novel biomarkers for cancer diagnosis

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