Milk-clotting protease production by Nocardiopsis sp. in an inexpensive medium

Abstract

The milk-clotting and proteolytic activities of extracellular enzyme preparations from Nocardiopsis sp. were investigated under different culture conditions. A soybean flour medium was used, with concentrations of soybean flour and of glucose varying from 0.25 to 1% w/v and from 0 to 1% w/v, respectively. Growth was monitored with 2 ml samples withdrawn from the culture medium at 8-h intervals, for determination of total protein, proteolytic activity, milk-clotting activity and sugar reduction. The best milk-clotting protease production, with a specific activity of 24.49 U/mg at 40 h, was obtained in the glucose-free medium containing soybean flour 1% w/v.

Optimization of the production and characterization of milk clotting enzymes by Bacillus subtilis natto

“…subtilis (natto) Takahashi. However, the requirement of carbon source for MCE production varied depending on the microorganism used (Abdel-Fattah & Saleh 1979 ; Cavalcanti et al 2005 ; Hashem 1999 ; Shieh et al 2009 ). Therefore, starch (X 1 ), corn steep powder (X 2 ), soybean meal (X 3 ) and dry milk (X 4 ) were the major variables in M1 affecting the performance of the culture in terms of MCE production, and they were chosen for optimization by RSM; the inorganic nitrogen sources were omitted for further study.…”

Role of casein on induction and enhancement of production of a bacterial milk clotting protease from an indigenously isolated Bacillus subtilis

“…1964; D’Souza and Pereira 1982) and others like Myxococcus (Poza et al. 2003) and Nocardiopsis (Cavalcanti et al. 2005) have also been reported as producers.…”

Production of Milk-Clotting Protease from Bacillus subtilis

“…Though, rice bran has been reported by Thakur et al [14] for mcp production from Mucor, but maximum yield was obtained only with wheat bran. Soybean meal has been reported to be a supporter of MCA by Cavalcanti et al [1] but in submerged fermentation only. Replacing phosphate buffer with a salt solution increased mcp production in both rice bran and soybean meal by 2.06-and 2.17-folds, respectively (Fig.…”

“…Shortage of animal rennet supply has led to tremendous efforts in seeking out alternatives to calf rennet [1]. Enzymes from microorganisms like Mucor miehei, Mucor pusillus, Endothia parasitica, Irpex lacteus, and Aspergillus niger have replaced calf chymosin in commercial cheese making [2].…”