Mikrokinematographische Studien �ber die Entstehung von Riesenzellen durch Herpes-B-Virus in Zellkulturen

Falke, D.; Richter, I. E.

doi:10.1007/bf01241249

Cited by 26 publications

(2 citation statements)

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“…Intermediate fiber systems are still surrounding the original nuclei after fusion, as it is suggested by earlier observations (3). During infection with fusion or rounding producing strains of HSV the keratin fiber system seems to become condensed, thickened and deposited at the nuclear membrane.…”

Section: Arrangement Of Keratin Vimentin and Tubulin In Cells Whim Rmentioning

confidence: 73%

Microtubules and intermediate filaments of herpes simplex virus infected cells

Dienes

Müller²,

Falke³

1987

Archives of Virology

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The fate of microtubules and of vimentin or keratin containing intermediate filaments during infection with fusion or rounding producing strains of herpes simplex virus (HSV) was investigated. Microtubules polymerize early after fusion of cells. However, they do not reconstitute 6-7 hours post infection (p.i.) after release of a colcemid block. Keratin and vimentin are maintained around the original nucleus still inside of recruited cells in the polykaryocyte. Cells of fibroblastic and epithelial origin fuse. Inside of polykaryocytes keratin or vimentin containing fibers seem to polymerize. Keratin is to be found in invaginations in the nuclei surrounded by the inner layer of the nuclear membrane. Anti-keratin antibodies specifically label HSV envelopes located in the cytoplasm or outside of the cell. Controls of the procedure allowed to exclude labelling of HSV envelopes via gpE, which represents HSV induced Fc receptors. Late stages of infected cells contain thickened and condensed keratin fibers. Conversely, vimentin fibers late after infection appear to be evenly distributed and to be thin. Microtubules decay late after infection with rounding producing strains of HSV, whereas keratin and vimentin fibers are still present late after infection.

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Section: Arrangement Of Keratin Vimentin and Tubulin In Cells Whim Rmentioning

confidence: 73%

Microtubules and intermediate filaments of herpes simplex virus infected cells

Dienes

Müller²,

Falke³

1987

Archives of Virology

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“…Amitofic nuclear division has not been observed. Time-lapse studies of cells infected with parainfluenza virus 3 (19,20), measles virus (21), and herpes B virus (51,52), and autoradiographic studies of measles-infected amnion cells (53) have indicated that giant cells are formed by fusion. Herpes simplex virus also induces cell fusion, but the course of giant cell formation may differ from that found with SVS.…”

Section: Discussionmentioning

confidence: 99%

On the Role of the Response of the Cell Membrane in Determining Virus Virulence

Holmes

Choppin

1966

The Journal of Experimental Medicine

161

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The simian myxovirus SV5 multiplies in a continuous line of baby hamster kidney (BHK21-F) cells causing extensive cell fusion, followed by cell death. After inoculation of 15 PFU/cell, the latent period was 7 hr, the doubling time approximately 60 min, and the yield 7 PFU per cell. Giant cell formation began about 6 hr after infection and rapidly progressed to the formation by 14 to 18 hr of a single syncytium which disintegrated by 24 to 36 hr. In contrast, SV5 multiplies in primary rhesus monkey kidney cells for long periods of time producing high yields of virus with little cytopathic effect. High multiplicities of SV5 induced cell fusion in BHK21-F cells within 1 hr in the absence of virus multiplication but had no visible effect on monkey kidney cells. Time-lapse photomicrography has demonstrated that giant cells form by fusion of infected cells, and that some polykaryocytes divide. During aberrant division of polykaryocytes giant nuclei are formed from the nuclear material of several parent nuclei. The cytoplasmic development of viral antigens as demonstrated by immunofluorescence is similar in BHK21-F and monkey kidney cells. Synthesis of cellular DNA, RNA, and protein in monkey kidney cells is not shut off by SV5-infection, and in BHK21-F cells synthesis of these macromolecules is not inhibited until after extensive cell fusion has occurred 12 to 15 hr after infection. Persistently infected BHK21-F and monkey kidney cells have been serially carried through 11 and 28 cell passages, respectively. The results suggest that whether SV5 acts as a moderate virus, as in monkey kidney cells, or a virulent virus, as in BHK21-F cells, depends on the response of the cell membrane to the virus.

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Makrophagen: Herkunft, Entwicklung und Funktion

Roos¹

1970

Immunreaktionen / Immune Reactions

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Mikrokinematographische Studien �ber die Entstehung von Riesenzellen durch Herpes-B-Virus in Zellkulturen

Cited by 26 publications

References 0 publications

Microtubules and intermediate filaments of herpes simplex virus infected cells

Microtubules and intermediate filaments of herpes simplex virus infected cells

On the Role of the Response of the Cell Membrane in Determining Virus Virulence

Makrophagen: Herkunft, Entwicklung und Funktion

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