Migration of the primordial germ cells and gonad formation in the early chicken embryo

Hong, Ying; Seo, Dong Sam; Jeong, Dong Kee; Choi, Kyunghee; Han, Jinnuo

doi:10.5713/ajas.1995.557

Cited by 9 publications

(8 citation statements)

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“…Starting on day 6 of embryonic development, the number of gonadal PGCs significantly increased (P<0.05) and reached the peak number of gonadal PGCs on the embryonic development on day 7, which was probably that all PGCs might already reach the embryonic gonad as reported by Hong et al (1995). Furthermore, this opinion was supported by Nakamura et al (1988) that the PGCs had mostly settled down in the gonadal ridge on the day 7 th of the incubation period.…”

Section: Number Of Gonadal Pgcssupporting

confidence: 70%

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Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken

Sopiyana¹,

Setiadi

Fahrudin

et al. 2017

Med Pet

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Primordial germ cells (PGCs) are cells that will differentiate themselves into spermatogonia in the testis or oogonia in the ovary. Primordial germ cells arise from epiblast and circulate through the bloodstream and finally entering gonadal anlage. The aim of this study was to determine the number of gonadal PGCs of KUB chicken at different development stages. Sixty KUB chicken fertile eggs were divided into four groups (6, 7, 8, and 9 days incubation periods), and incubated at 38 o C with a humidity of 60%. Harvesting was synchronized to the embryonic development at 6-9 d. Gonads were collected using sharp tweezers, and were placed in Eppendorf tube 1.5 mL containing 500 µL PBS [-]. Gonadal PGCs were purified using PBS [-]. The results showed that the average number of gonadal PGCs at 6, 7, 8, and 9 d were 113.7; 143.5; 92.9; and 85.7 cells per embryo, respectively. Number of gonadal PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P<0.05), which reached a peak at day 7 of incubation, so that the isolation and collection of PGCs from the gonads were recommended at day 7 of incubation. This information is useful in production of germline chimera of other Indonesian local chickens. Keywords

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Section: Number Of Gonadal Pgcssupporting

confidence: 70%

“…The reason of the decreased number of PGCs on the day 8th of incubation might be due to the start of PGCs to differentiate sexually to be a female embryo (Hong et al, 1995). Furthermore, PGCs began the meiotic division actively forming oogonia on days 8-9 of the incubation period (Van Krey, 1990).…”

Section: Number Of Gonadal Pgcsmentioning

confidence: 99%

Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken

Sopiyana¹,

Setiadi

Fahrudin

et al. 2017

Med Pet

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“…[6] The PGCs are founder cells of the germ line and their descendants will form the functional gametes of the adult animal. [7] The two basic roles of the ovary are to deliver the ova and to function as an endocrine organ by the production of ovarian steroids. [8] Although vertebrates including the chicken display a superficial bilateral symmetry, most of the internal organs including the ovaries develop and locate with a consistent Left: Right (L: R) gonadal asymmetry as only the left gonad and left oviduct fully developed.…”

Section: Introductionmentioning

confidence: 99%

Pre-Hatching Development of the Alexandria Chicken Ovaries

Alsafy¹,

Samir²,

Gendy³

et al. 2017

aanat

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Background: The aim of this study was to explore the ultrastructure changes of the left and right ovaries at the pre-hatching period of Alexandria strain of chicken. Methods: One hundred fertilized egg of Alexandria breed of chicken used. The whole embryo has undergone the light microscopic examination at HH20 (E.3), HH21 (E.3.5), HH23 (E.4), HH29 (E.6) and HH34 (E.8). The ovary has undergone the light microscopic examination at HH38 (E.12) and HH42 (E.16), SEM at HH26 (E.5), HH29 (E.6), HH36 (E.10), HH38 (E.12), HH39 (E.13) and HH42 (E.16), TEM at HH38 (E.12) and HH42 (E.16). The genital ridge appeared by a thickening of the coelomic epithelium medio-ventral surface of the developing mesonephroi at HH20 (E.3). The boundaries of the undifferentiating gonads defined clearly separated from the mesonephroi. The undifferentiated gonads bulged as a distinct organ in the coelomic cavity at HH23 (E.4). Results: At the initial stages of the gonadogenesis, the germinal epithelium was stratified squamous epithelium. The PGCs appeared at the genital ridge at HH21 (E.3.5). The PGCs viewed at the dorsal mesentery with few microvilli and showed positive PAS reaction because of the glycogen content in their cytoplasm. The left-right gonadal asymmetry firstly detected by the number of PGCs migrating toward the left gonadal ridge more than the right at HH20 (E.3) and the macroscopic examination of gonadal asymmetry began at HH34 (E.8). Conclusion: The left ovary appeared a smooth rodshape, its stroma showed lipid droplets and its parenchyma showed an extensive arrangement of interstitial cords at HH42 (E.16).

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“…On the other hand, the chicken primordial germ cells (PGCs) were also used as a vector. Since the PGCs are progenitor cells of ova or spermatozoa (Kuwdna, 1993;Han et al, 1994;Hong et al, 1995), it was considered to produce germ-line transgenic chickens more easily that may produce spermatozoa or ova carrying foreign gene. Judging from the above-mentioned, it has been thought that germline transgenic chicken may also produce transgenic offspring.…”

Section: Introductionmentioning

confidence: 99%

Successful Production of Transgenic Chicken by DNA Injection into Germinal Crescent Region (GCR) of Chicken Embryos

Morimoto

Arima

Furuta

et al. 2002

Journal of Applied Animal Research

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Migration of the primordial germ cells and gonad formation in the early chicken embryo

Cited by 9 publications

References 0 publications

Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken

Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken

Pre-Hatching Development of the Alexandria Chicken Ovaries

Successful Production of Transgenic Chicken by DNA Injection into Germinal Crescent Region (GCR) of Chicken Embryos

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