Migration of Neural Stem Cells in Hippocampal Slices in Hypoxia Modeling

Stukach, Yulia; Gainutdinov, Khalil x; Dosina, Margarita; Pashkevich, S. G.; Андрианов, В. В.; Denisov, A. A.; Bogodvid, Tatiana; Yafarova, G. G.; Bushuk, Sergei; Tatiana, Kuznetsova; Kulchitsky, Vladimir A.

doi:10.28967/jscrt.2016.01.16002

Cited by 3 publications

(5 citation statements)

References 15 publications

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“…Technique of MSC implantation into nasal submucosa was chosen due to minimization of side effects and simplicity of accomplishment by neurosurgeon [8,12,[20][21][22]. Migratory abilities of SC were taken into account during technique development [5,6,8,9,11,12]. Moreover, the presence of endogenous SC in nasal mucosa says for MSC implantation namely in that receptive field [8][9][10][11].…”

Section: Methodsmentioning

confidence: 99%

“…Authors started conducting studies for assessment of SC perineural implantation significance in activation of reparative processes about 10 years ago [5][6][7][8][9][10][11][12]. Patterns of SC migration in brain tissue have been established in the experiments -depending on the area of SC injection at cranial nerve peripheral endings and localization of neurodestruction site in brain tissue [5,[10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Such principle of migration was determined as somatotopic distribution of SC in brain [12]. SC consistently move directly to the site of destruction in brain [5][6][7][8][9][10][11][12]. It is already proved nowadays that such aimed migration is determined by various signaling molecules which are expressed by nerve and glial cells in the area of neurodestruction [5][6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…SC consistently move directly to the site of destruction in brain [5][6][7][8][9][10][11][12]. It is already proved nowadays that such aimed migration is determined by various signaling molecules which are expressed by nerve and glial cells in the area of neurodestruction [5][6][7][8][9][10][11][12]. Somatotopic distribution of implanted SC in brain depending on the site of injection is another one regularity [12].…”

Section: Introductionmentioning

confidence: 99%

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Prospects of Perineural Implantation of Stem Cells in Recovery of Neural Network’s Functions in Brain Diseases

Vladimir

Alexandra

Yuri

et al. 2018

BJSTR

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Background:The question on prospects of additional use of cellular therapy in standard protocols of brain neural networks recovery after neurodestructive processes of various etiology was analyzed. Data on low efficiency of existing methods of neurodestructive processes treatment gave a boost to authors to conduct series of studies related to brain diseases and cellular technologies. Existing treatment principles now should be updated with new methods -in particular, cellular technologies. This statement was based on stem cells (SC) presence in human brain. Nature has formed basis for neural network formation in the process of training and for recovery therapy in case of pathology. Unfortunately, recovery potential of brain SC appeared to be ineffective in neurodestructive processes. Therefore, SC should be additionally injected into brain. It is advisable to use autologous SC in order to avoid possible side effects, such as malignization of allogeneic and other SC. But in certain cases, use of allogeneic SC can be approved.Methods: Autologous mesenchymal SC (MSC) are usually administered to patients in experimental and clinical conditions. Two ways of MSC delivery to brain are preferred: with cerebral blood flow after MSC injection into bloodstream and MSC implantation close to damaged area after additional skull trepanation. Authors experimentally developed and clinically evaluated new technique of perineural implantation of MSC. This method is based on natural ability of SC to migrate. MSC are implanted into the area of cranial nerve endings (mainly olfactory and trigeminal) and then they migrate along cranial nerves to cranial cavity.Results: The area of vomeronasal complex (VC) is preferred for MSC implantation because its mucosa has nerve endings of both olfactory and trigeminal nerves. Such technique of MSC implantation was substantiated by authors and was named perineural implantation of MSC when used in neurodestructive processes. Discussion and Conclusion:The article contains critical analysis and prospects of cellular technologies development for therapy of brain diseases. Special attention is paid in discussion to technology of perineural implantation of MSC in experimental and clinical conditions.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prospects of Perineural Implantation of Stem Cells in Recovery of Neural Network’s Functions in Brain Diseases

Vladimir

Alexandra

Yuri

et al. 2018

BJSTR

View full text Add to dashboard Cite

show abstract

“…Preservation of blood supply to the brainstem structures under model experimental conditions is a condition for the functioning of the neural and glial structures of the brainstem. It has been demonstrated [27] that MSCs migrate mainly to damaged areas of the nervous tissue and, therefore, the main migration of stem cells is directed to the cortical regions of the brain, which are supplied with blood from the region of clipped carotid arteries.…”

Section: Migration Of Mscs After Its Intranasal Perineural Administrationmentioning

confidence: 99%

Effects of Perineural Stem Cell Implantation on Motor Activity and Content of NO and Copper in the Olfactory System After Brain Ischemia

Андрианов

Yafarova

Tokalchik

et al. 2021

Advances in Cognitive Research, Artificial Intelligence and Neuroinformatics

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It was shown by electron paramagnetic resonance (EPR) spectroscopy that one day after bilateral occlusion of the common carotid arteries (to simulate ischemia) in Wistar rats (group 1, n = 15) under ketamine-xylazine-acepromazine anesthesia (55.6, 5.5, 1.1 mg/kg, respectively, intraperitoneally), the content of nitric monoxide (NO) in the olfactory bulbs decreased, and recovered after two days. A similar trend was observed in Wistar rats (group 2, n = 15), which, simultaneously with ischemia modeling, were implanted intranasally with mesenchymal stem cells (MSCs). An intact group of rats (group 3, n = 15) was kept in the usual conditions of the vivarium and did not undergo surgical interventions. The content of copper in the olfactory bulbs of rats (for assessing the activity of superoxide dismutases 1 and 3) after ischemia modeling (group 1) tended to increase and maintain the increase for two days of observation. Intranasal administration of MSCs during the ischemia modeling was accompanied by a more significant increase in copper content on the first day after brain ischemia modeling, but after two days, a tendency towards restoration of the initial copper level was noted.Intranasal administrating of MSC simultaneously with ischemia modeling (group 2) was accompanied by a more rapid recovery of orientational-motor activity in experimental animals compared to rats in which only modeling of ischemic stroke was performed (group 1).

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Content of nitric oxide and copper in the olfactory bulbs of the rats brain after modeling of cerebral stroke and intranasal administration of mesenchymal stem cells

Андрианов

Кульчицкий

Яфарова

et al. 2021

Regional blood circulation and microcirculation

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Introduction. With a decrease in the oxygen content in the inhaled air, violations of the cerebral blood flow, brain ischemia occurs, which can end in an ischemic stroke. Aim. Comparative analysis of the intensity of nitric oxide (NO) production and the copper content in the olfactory bulb tissues of the brain of male Wistar rats after modeling an ischemic stroke. Materials and methods. Modeling of ischemic stroke by ligation at the bifurcation level of both common carotid arteries and measuring the content of NO and copper by EPR spectroscopy. Results. The relative changes in the number of NO-containing complexes and the copper content were estimated from the integrated signal intensity of the complexes (DETC)2-Fe2+-NO and (DETC)2- Cu. A significant decrease by 47 % after 1 and 57 % after 2 days, respectively, in the NO content in the olfactory bulb of the rat brain was found after the ischemia modeling. The level of NO production in rats that underwent ischemia simulation with simultaneous intranasal administration of mesenchymal stem cells (MSCs) was also reduced by 51 % after 1 and 70 % after 2 days, respectively, after ischemia modeling. There was no significant difference in the NO content in the rats after ischemia modeling with simultaneous intranasal administration of MSCs compared to the ischemic rats. The copper content, which corresponds to the level of superoxide dismutase 1 and 3, in the rat’s olfactory bulb tended to increase after ischemia modeling and it persisted for two days of observation (an increase of 50 % in both cases). Intranasal administration of MSCs was accompanied by a significant increase in the Cu content (by 89 %) 1 day after the ischemia modeling, and 2 days later – by a decrease in its content by 36 % (compared to the control). In the control animals that were not subjected to surgical operations, no changes in the content of NO or copper were observed. Conclusion. The experiments showed a 2-fold decrease in the NO content in the olfactory bulb of the rat brain 1 and 2 days after the ischemia modeling, and demonstrated that the intranasal administration of MSCs did not affect the intensity of NO production on the 1st and 2nd days after the brain ischemia modeling, but was accompanied by an increase in the antioxidant protection of the nervous tissue one day after ischemia.

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Migration of Neural Stem Cells in Hippocampal Slices in Hypoxia Modeling

Cited by 3 publications

References 15 publications

Prospects of Perineural Implantation of Stem Cells in Recovery of Neural Network’s Functions in Brain Diseases

Prospects of Perineural Implantation of Stem Cells in Recovery of Neural Network’s Functions in Brain Diseases

Effects of Perineural Stem Cell Implantation on Motor Activity and Content of NO and Copper in the Olfactory System After Brain Ischemia

Content of nitric oxide and copper in the olfactory bulbs of the rats brain after modeling of cerebral stroke and intranasal administration of mesenchymal stem cells

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