Migration behavior and separation of benzenediamines, aminophenols and benzenediols by capillary zone electrophoresis

Lin, Chin‐Feng; Chen, Yu-Tai

doi:10.1016/s0021-9673(99)00937-1

Cited by 34 publications

(21 citation statements)

References 25 publications

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“…Several modifications have been suggested to these classical relationships, but in our opinion the classical formulations still providing the best results in the whole pH range [9,10,16]. In contrast to the extensive work with peptides in aqueous media, the literature for other compounds or solvents is scarce despite the assumptions made for the development of these relationships may be also applicable to other structures or solvents [19][20][21]. Table 2 shows the parameters resulting from the linear correlation of m e and M/q a (a 5 1/3, for the Stoke's law, a 5 1/2 for the classical polymer model and a 5 2/3 for Offord's law) for the studied quinolones between pH 2 and 11 in water and ACN-water mixtures.…”

Section: Resultsmentioning

confidence: 99%

“…It has been demonstrated that if accurate acidity constant values are available, the electrophoretic separations can be easily predicted taking into account the classical semiempirical relationships between electrophoretic mobility and charge-to-mass ratio (m e versus q/M a ) [9,10,16]. The literature about this topic for analytes different from peptides and proteins is scarce and to the best of our knowledge no papers are reporting the study of these relationships in hydroorganic media [19][20][21]. The addition of appropriate organic solvents to the background electrolytes it is specially useful to increase solubility of sparingly water-soluble drugs and, in some cases, leads to an improvement of detection sensitivity and separation resolution [22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

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Modeling the electrophoretic behavior of quinolones in aqueous and hydroorganic media

et al. 2010

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The applicability of the classical semiempirical relationships between the electrophoretic mobility and charge-to-mass ratio (m e versus q/M a ) has been investigated to describe migration behavior of a series of quinolones in aqueous and hydroorganic media. The Stoke's law (a 5 1/3), the classical polymer model (a 5 1/2) and the Offord's surface law (a 5 2/3) were evaluated in the pH range comprised between 2 and 11 in ACN-water mixtures of 0, 10 and 30% m/m ACN. As accurate acidity constant values were available for charge calculations, correlations were excellent. The results obtained using the Offord's surface law were slightly better and the plots of q/M 2/3 against the separation electrolyte pH were used to select the optimum pH values for separation of a quinolone mixture. The electropherograms simulated considering the accurate charge-to-mass ratio of each quinolone at the selected pH values were in good agreement with the experimental results. The ability of these relationships to predict the separation of the studied quinolones at a certain pH value in methanol-water and THF-water mixtures was also demonstrated.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modeling the electrophoretic behavior of quinolones in aqueous and hydroorganic media

et al. 2010

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“…CEMD can obtain electric flux (Q) in addition to the common electric current. With a laboratory-edited workstation, CEMD produces diffusivity spectrum and, if [38][39][40]), Q-or t-electropherogram. In this study, the diffusivity spectrum was plotted by peak area versus diffusivity, much the same as MS. Diffusivity value can thus directly be read from the spectrum.…”

Section: Introductionmentioning

confidence: 99%

Electromigration diffusivity spectrometry: A way for simultaneous determination of diffusion coefficients from mixed samples

et al. 2010

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A novel method was proposed for simultaneous measurement of diffusion coefficients, (D), from mixed samples by electrophoresis and termed electromigration-based diffusivity spectrometry. After theoretical treatment, D-equation for practical use has been deduced. With a modified CE system built in laboratory, electromigration-based diffusivity spectrometry has been realized and validated to suit for fast and accurate determination of diffusivities of mixed aromatic amino acids, phenols and aromatic organic acid, giving diffusivity spectra by peak area versus D, much similar to mass spectra. The precision of the measurement was found to critically depend on pH value of running buffer, which should be so selected that the analytes and internal standards could be charged at above 0.5e. The standards have to be selected at an electric flux far from each other and from analytes. In these cases, sample and running buffer concentrations, voltage and system temperature were found to have only negligible impact on the determination. In our test, the obtained measuring precision was generally kept within 1% for five runs, and the measured values of D agreed well with those from literature, with a deviation of less than 2.2% after the right use of calibration standards.

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“…Various methods are available for the determination of benzenediols or benzenetriols, including chromatographic [7][8][9][10][11], spectrophotometric analyses [12][13][14][15] and chemiluminescent (CL) detection [16][17][18]. Spectrophotometric methods are employed mainly for the determination of total amount of phenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

Post-column detection of benzenediols and 1,2,4-benzenetriol based on acidic potassium permanganate chemiluminescence

Fan

Zhang

Lin

2006

Talanta

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Migration behavior and separation of benzenediamines, aminophenols and benzenediols by capillary zone electrophoresis

Cited by 34 publications

References 25 publications

Modeling the electrophoretic behavior of quinolones in aqueous and hydroorganic media

Modeling the electrophoretic behavior of quinolones in aqueous and hydroorganic media

Electromigration diffusivity spectrometry: A way for simultaneous determination of diffusion coefficients from mixed samples

Post-column detection of benzenediols and 1,2,4-benzenetriol based on acidic potassium permanganate chemiluminescence

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