Mid-term effect of stem cells combined with transmyocardial degradable stent on swine model of acute myocardial infarction

Luan, Yun; Liu, Xiaocheng; Zhang, Guangwei; Shi, Rui; Zhao, Xia; Chun-hua, Zhao; Tian-jun, Liu; Lu, Feng Hwa; Yang, Qin; He, Guo‐Wei

doi:10.1097/mca.0b013e328338cc94

Cited by 8 publications

(11 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Following primary culture for 4 days, the mini-swine BMSCs appeared as colonies of large flat cells or spindle-like cells, and the cells were attached to the culture dish tightly and proliferated rapidly in the culture medium (10). FACS analysis indicated that the cells did not express the endothelial marker CD31.…”

Section: Resultsmentioning

confidence: 99%

“…A mini-swine model of AMI was generated by ligating the left anterior descending artery (LAD) using the method of a previous study (10): Under general anesthesia, animals were intubated and positive pressure ventilation was maintained. The middle third of the LAD was ligated following three intermittent brief preconditioning occlusions, each for 5 min.…”

Section: Acute Myocardial Infarction Model and Stem Cell Transplantatmentioning

confidence: 99%

“…Glyceraldehyde-3-phosphate dehydrogenase was used as an internal control for RNA input level. The sequences of primers and reaction conditions are described in our previous study (10). Following RT-PCR, 1% agarose gel electrophoresis was performed and the UVI pro gel documentation system (Uvitec, Cambridge, UK) was used for semi-quantification analysis of each PCR product.…”

Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

“…RT-PCR analysis was performed to detect the relative pulmonary expression levels of vascular endothelial growth factor (VEGF), vWF, transforming growth factor-β3 (TGF-β3) and interleukin-1β (IL-1β) as previously described (10). Glyceraldehyde-3-phosphate dehydrogenase was used as an internal control for RNA input level.…”

Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

See 3 more Smart Citations

Bone marrow mesenchymal stem cells improve myocardial function in a swine model of acute myocardial infarction

Zhao

Liu

Kong

et al. 2014

Molecular Medicine Reports

Self Cite

View full text Add to dashboard Cite

Abstract.The aim of the current study was to confirm the effect and elucidate the mechanism of bone marrow mesenchymal stem cells (BMSCs) in acute myocardial infarction (AMI). AMI was induced in mini-swine by ligating the left anterior descending coronary artery, and BMSCs (1x10 7 ) were injected via a sterile microinjection into the ischemic area. Six months postoperatively, electrocardiograph-gated single photon emission computed tomography revealed that the myocardial filling defect was reduced and the left ventricular ejection fraction was improved in the BMSC group compared with the control group (P<0.05). Histopathological examination indicated that, in the BMSC treatment group, the percentage of survived myocardial tissue and the vessel density were increased, and the percentage of apoptosis was decreased compared with controls (P<0.05). Reverse transcription-polymerase chain reaction results indicated that the expression levels of multiple inflammatory factors were significantly upregulated in the BMSC group compared with levels in the control group (P<0.05). In conclusion, the present study demonstrated that BMSC injection significantly improved cardiac function and reduced infarct size in six months, indicating that this method may be valuable for future study in clinical trials. IntroductionAccording to 2011 statistics, acute myocardial infarction (AMI) is one of the most prevalent causes of death and morbidity (1,2). Adult cardiomyocytes lack the capacity for regeneration, so scar tissue replaces lost myocardium following myocardial infarction, and this leads to cardiac remodeling with reduced left ventricular (LV) function (3). Cell therapy is a potential method for the regeneration and repair of myocardium and the improvement of myocardial function following ischemic injury (4). A number of cell types, including foetal Flk1 + CD34 + CD31 -bone marrow stem cells (BMSCs) have demonstrated cardiac regenerative capacity (5), however, the mechanism that stem cell transplantation utilizes in order to improve cardiac function following ischemic heart disease is unclear and there is little information available regarding the medium-and long-term effects. On the other hand, there exist a variety of transplantation methods, including local intramyocardial injection, intravenous injection, and intracoronary injection to the infarct zone (6). Intramyocardial injection of BMSCs has been demonstrated to be safe, and to lead to reverse remodeling and improved contractile ability of scarred areas (3,7). The mechanisms by which BMSCs reduce infarct size and improve cardiac function in animal models are complicated, involving engraftment, differentiation into functional cardiomyocytes and paracrine signaling (8,9). In the present study, fetal Flk1 + CD34 + CD31 -BMSCs were transplanted via intramyocardial injection of the mini-swine, then the myocardial function, stem cell migration and extent of survival in the myocardium was evaluated. The current study was therefore designed to determine the long-term (6-month) e...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Acute Myocardial Infarction Model and Stem Cell Transplantatmentioning

confidence: 99%

Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

See 2 more Smart Citations

Bone marrow mesenchymal stem cells improve myocardial function in a swine model of acute myocardial infarction

Zhao

Liu

Kong

et al. 2014

Molecular Medicine Reports

Self Cite

View full text Add to dashboard Cite

show abstract

“…Для заселения in vitro тканеинженерных конструкций и стентов часто используют MSCs костного мозга благо-даря их доступности, способности к стабильной пролиферации [46][47][48][49] и сохранению мультипо-тентности даже после многократных пассажей [50]. Результаты экспериментов по имплантации стентов, предварительно заселенных MSCs кост-ного мозга, демонстрируют ускорение реэндоте-лизации, ингибирование гиперплазии интимы и снижение риска рестенозов [49].…”

Section: эндотелизация поверхности материала In Vitrounclassified

Surface Modification of Bare-Metal Stents for Preventing Restenosis (Part 1)

Лотков¹,

Матвеева²,

Антонова³

et al. 2017

Kompleks. probl. serdečno-sosud. zabol.

View full text Add to dashboard Cite

Рестенозы металлических стентов, возникающие после имплантации, остаются нерешенной проблемой и существенно ограничивают их терапевтическую эффективность. В обзоре освещены наиболее перспектив-ные с точки зрения физиологии и клеточной биологии варианты модификации металлических поверхностей, представлены преимущества и недостатки каждого из методов. В первую часть обзора включены направ-ления, касающиеся разработки стентов с антитромботическим и антипролиферативным покрытием, так же представлены наиболее значимые и интересные исследования, направленные на формирование на поверх-ности стентов эндотелиального слоя in vitro.Ключевые слова: металлические стенты, рестеноз, модификация поверхности, эндотелизацияThe therapeutic efficacy of bare-metal stents is significantly limited due to frequent restenosis. Here we review the approaches for surface modification to prevent restenosis, presented the advantages and disadvantages of each method. In the first part of the review, we consider antithrombotic and antiproliferative stents coating. The review presents the most significant and interesting research aimed forming endothelial layer on the stents surface in vitro.Keywords: bare-metal stents, restenosis, surface modification, endothelialization НИИ КПССЗ имеет большой опыт в разработке изделий для сердечно-сосудистой хирургии, в частности биопротезов кла-нов сердца и сосудов. С 2010 года под руководством академика РАН л. С. Барбараша начаты исследования по созданию био-зорбируемых сосудистых протезов малого диаметра. Основная цель -создание полимерных конструкций с бионаправленным йствием, способных заменить отдельные структуры живого организма, в частности сердечно-сосудистой системы. Научная но-зна поставленной задачи заключается в использовании нового подхода создания органа непосредственно в организме пациента счет биофункциональности и биорезорбируемости полимерных конструкций. доказана долгосрочная проходимость полимерных судистых графтов на основе поликапролактона и композиции полигидроксибутирата/валерата и поликапролактона. В экспери-нтах in vitro доказано, что ростовые факторы, инкорпорируемые в состав биодеградируемых графтов, сохраняют свою био-гическую активность. В долгосрочных экспериментах in vivo доказано, что сосудистый эндотелиальный фактор роста ускоряет дотелизацию и улучшает проходимость биодеградируемых полимерных сосудистых графтов.Ключевые слова: биодеградируемые полимеры, сосудистый графт, ростовые факторы. DEVELOPMENT OF TISSUE ENGINEERED SMALL DIAMETER VASCULAR GRAFT FOR THE CARDIOVASCULAR SURGERY NEEDS L. V. ANTONOVA, YU. A. KUDRYAVTSEVA Federal State Budgetary Scientific Institution Research Institute for Complex Issues of Cardiovascular Diseases. Kemerovo, RussiaRICICd has large experience in the development of products for cardiovascular surgery, in particular, the bioprosthesis heart valves d blood vessels. Since 2010, under the leadership of the academician of RAS L. S. Barbarash researches to create bioresorbable scular prostheses of small diameter began. the primary purpose -to cre...

show abstract

Mesenchymal stem cell prevention of vascular remodeling in high flow-induced pulmonary hypertension through a paracrine mechanism

Luan

Zhang

Kong

et al. 2012

International Immunopharmacology

Self Cite

View full text Add to dashboard Cite

Mid-term effect of stem cells combined with transmyocardial degradable stent on swine model of acute myocardial infarction

Cited by 8 publications

References 44 publications

Bone marrow mesenchymal stem cells improve myocardial function in a swine model of acute myocardial infarction

Bone marrow mesenchymal stem cells improve myocardial function in a swine model of acute myocardial infarction

Surface Modification of Bare-Metal Stents for Preventing Restenosis (Part 1)

Mesenchymal stem cell prevention of vascular remodeling in high flow-induced pulmonary hypertension through a paracrine mechanism

Contact Info

Product

Resources

About