Mid-Term Clinical Results Using a Tissue-Engineered Pulmonary Valve to Reconstruct the Right Ventricular Outflow Tract During the Ross Procedure

Dohmen, Pascal M.; Lembcke, Alexander; Holinski, Sebastin; Kivelitz, Dietmar; Braun, J.; Pruß, Axel; Konertz, Wolfgang

doi:10.1016/j.athoracsur.2007.04.072

Cited by 110 publications

(78 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our group has already reported on clinical experience with in-vivo-developed TE pulmonary heart valves, based on decellularized xenograft matrices 3, 8 and the creation of decellularized hearts as potential neoscaffolds for whole heart TE. 9 The present investigation was based on total decellularization of human heart valve scaffolds, dissected from explanted hearts of heart transplant recipients.…”

Section: Discussionmentioning

confidence: 99%

“…11 Various models featuring polymer biodegradable, biological allogeneic, and xenogeneic decellularized scaffolds have been already developed. 5,12-15 Our group already reported the successful in vivo application of TE pulmonary heart valves based on decellularized xenograft matrices 3, 8 and generation of 3-dimensional myocardial neoscaffolds by decellularization of whole hearts. 9 In this paper, we will present the in vitro TE of human heart valve neoscaffolds using human umbilical cord endothelial cells (HUVECs) under simulated physiological conditions.…”

Section: Neering (Te)mentioning

confidence: 99%

See 1 more Smart Citation

Reendothelialization of Human Heart Valve Neoscaffolds Using Umbilical Cord-Derived Endothelial Cells

Weymann

Schmack

Okada

et al. 2013

Circ J

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Neering (Te)mentioning

confidence: 99%

Reendothelialization of Human Heart Valve Neoscaffolds Using Umbilical Cord-Derived Endothelial Cells

Weymann

Schmack

Okada

et al. 2013

Circ J

View full text Add to dashboard Cite

“…Natural tissuederived valve scaffolds possess desirable 3-dimensional architecture, mechanical properties, and potentially adhesion/ migration sites capable of promoting cell attachment and ingrowth. Decellularized tissue scaffolds derived from valve (in some cases with in vitro "reendothelialization" performed before implantation of the valve) have been used in clinical studies in the pulmonary position, 133,134 but decellularized porcine valves implanted in humans as AV replacements elicited a strong inflammatory response and suffered structural failure, which has inhibited further use. 135 Cell-free porcine small intestinal submucosa has been investigated experimentally as a valve cusp material.…”

Section: Schoenmentioning

confidence: 99%

Evolving Concepts of Cardiac Valve Dynamics

2008

View full text Add to dashboard Cite

Abstract-Considerable progress has been made in recent years toward elucidating a conceptual framework that integrates the dynamic functional structure, mechanical properties, and pathobiological behavior of the cardiac valves. This communication reviews the evolving paradigm of a continuum of heart valve structure, function, and pathobiology and explores its implications. Specifically, we discuss (1) the interactions of valve biology and biomechanics (eg, correlations of function with structure at the cell, tissue, and organ levels and mechanical considerations, development, endothelial cell and interstitial cell biology, extracellular matrix biology, homeostasis, and adaptation to environmental change); (2) Key Words: aortic valve Ⅲ mitral valve Ⅲ pathology Ⅲ prosthesis Ⅲ tissue engineering I mportant conceptual advances, new data, and evolution in our understanding and application of the principles underlying the dynamic functional, biological, and mechanical behavior of the cardiac valves have occurred in recent years. Research in heart valve biology and disease has been enabled by the availability of cultures of heart valve cells, computational methodology, the design and use of in vitro and in vivo experimental systems that model elements of valve biological and pathobiological activity, and targeted study of normally functioning and pathological native and substitute human valves. Collectively, these developments have facilitated a growing understanding of how shortand long-term biomechanical valve function at the organ level relates to tissue and cell structure and normal valve function, elucidated the pathological anatomy and mechanisms of valvular dysfunction, fostered improvements in tissue heart valve substitutes and surgical repairs, and informed innovative approaches to heart valve tissue engineering and regeneration. [1][2][3][4] In this communication, we highlight key recent insights into heart valve function and dysfunction and their implications for the prevention, diagnosis, and treatment of clinical heart valve disease, currently and in the future. The heart valves are tissue structures whose motions are driven by mechanical forces exerted by the surrounding blood and heart. The ability of the valves to permit unobstructed forward flow depends on the mobility, pliability, and structural integrity of their leaflets (in the TV and MV) and cusps (in the PV and AV).The individual AV cusps attach to the aortic wall in a crescentic (or semilunar) fashion, ascending to the commissures (where adjacent cusps come together at the aorta) and descending to the basal attachment of each cusp to the aortic wall. Behind the cusps are dilated pockets in the aortic root, called sinuses of Valsalva, which bulge with each ejection of blood. The AV cusps and their respective sinuses are named for their relationship to the coronary artery ostia that arise from them, normally a left, a right, and a noncoronary (cusp and associated sinus). In the middle of the free edge of each cusp on the ventricular surface is a ...

show abstract

“…26 Many previous studies have suggested that the ECM may provide a suitable environment for repopulation by host cells, which would provide the ECM with a regenerative capacity typical of native valves, thus improving xenograft durability and preventing thrombosis. 14,15,29,30 However, further studies are required to investigate the capacity of the matrix to be repopulated with host endothelial and interstitial cells.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Abdolghafoorian

Farnia²,

Rs³

et al. 2017

Exp Clin Transplant

View full text Add to dashboard Cite

Objectives: Endothelial cells harbor many antigenic determinants that may be targets for the immune system. The aim of this study was to determine the immunologic effects of decellularization, using 3 different methods, on xenograft rejection. Materials and Methods: In a sterile plate containing phosphate-buffered saline, fresh sheep aortic heart valves were decellularized using 3 different enzymatic methods: with 900 µg/mL of collagenase at 40°C (method A), with 450 µg/mL of collagenase at 4°C (method B), and with 900 µg/mL of collagenase at 4°C (method C). Intact and decellularized valves were implanted subdermally into inbred male albino rabbits and extracted after 21 days (extra valve pieces were also extracted after 60 days, as control samples, for assessing chronic rejection). Valves were histologically analyzed for inflammatory cell infiltration. Subendothelial structure integrity was determined using surface electron microscope. Results: No inflammatory cell infiltration was seen around the decellularized valve with method A, and no subendothelial structure change was observed by surface electron microscope. Infiltration of immune cells involved in rejection was not seen around valves decellularized with method B, although the subendothelial structure was relatively preserved and valve stiffness was increased. With method C, we observed a foreign body-type reaction around the intact valve and the decellularized valve. Conclusions: Method A is considered the optimal method of decellularization in our study, as this method significantly reduced the immune response to xenograft tissue, while maintaining subendothelial tissue.

show abstract

Mid-Term Clinical Results Using a Tissue-Engineered Pulmonary Valve to Reconstruct the Right Ventricular Outflow Tract During the Ross Procedure

Cited by 110 publications

References 25 publications

Reendothelialization of Human Heart Valve Neoscaffolds Using Umbilical Cord-Derived Endothelial Cells

Reendothelialization of Human Heart Valve Neoscaffolds Using Umbilical Cord-Derived Endothelial Cells

Evolving Concepts of Cardiac Valve Dynamics

Untitled

Contact Info

Product

Resources

About