“Mid-G” Region Sequences of the Glycoprotein Gene of Austrian Infectious Hematopoietic Necrosis Virus Isolates Form Two Lineages within European Isolates and Are Distinct from American and Asian Lineages

Kolodziejek, Jolanta; Schachner, Oskar; Dürrwald, Ralf; Latif, Muna; Nowotny, Norbert

doi:10.1128/jcm.00566-07

Cited by 15 publications

(8 citation statements)

References 38 publications

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“…In our mid-G tree, where M genogroup isolates from Idaho and isolates from Columbia River basin were included, the European isolates did not cluster in any specific subgroup. Thus, in phylogenetic studies to date, European isolates of IHNV have been shown to cluster in the M genogroup with isolates from Idaho and Columbia River basin (the present study, Cozien & Thiéry 2003, Enzmann et al 2005, Kolodziejek et al 2008) and when full-length gene sequences are analysed, the European isolates form a distinct virus lineage (N gene in the present study, G and NV genes in Enzmann et al 2005). The finding that the Italian, Austrian and French isolates appear monophyletic with the German isolates suggests that there is a monophyletic origin for all IHNVs in Europe.…”

Section: Discussionsupporting

confidence: 52%

“…In 1987 IHNV was isolated from rainbow trout yolk sac fry in Italy (Bovo et al 1987) and from rainbow trout fry and fingerlings in France (Laurencin 1987). Since then isolation of IHNV in Europe has been reported from Austria (Kolodziejek et al 2008), Germany (Fichtner et al 2000, Enzmann et al 2005, Belgium (World Organisation for Animal Health 2007) and Switzerland (Knuesel et al 2003). The first outbreak of IHN in rainbow trout fry in Russia was in 2000 in Moscow province (Schelkunov et al 2001).…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

“…An earlier phylogenetic analysis of the partial G gene sequences (303 nucleotides [nt]) of IHNV isolates from North America included one index case isolate from France and one from Italy . In later studies the complete G and NV gene sequences were determined for 9 German isolates and the French index isolate (Enzmann et al 2005) and for a 615 bp region of the G gene of Austrian isolates (Kolodziejek et al 2008). Phylogenetic analysis in all these reports showed that the European isolates fell within the M genogroup.…”

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Genetic and serological typing of European infectious haematopoietic necrosis virus (IHNV) isolates

Johansson¹,

Einer-Jensen²,

Batts³

et al. 2009

Dis. Aquat. Org.

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Infectious haematopoietic necrosis virus (IHNV) causes the lethal disease infectious haematopoietic necrosis (IHN) in juvenile salmon and trout. The nucleocapsid (N) protein gene and partial glycoprotein (G) gene (nucleotides 457 to 1061) of the European isolates IT-217A, FR-32/87, DE-DF 13/98 11621, DE-DF 4/99-8/99, AU-9695338 and RU-FR1 were sequenced and compared with IHNV isolates from the North American genogroups U, M and L. In phylogenetic studies the N gene of the Italian, French, German and Austrian isolates clustered in the M genogroup, though in a different subgroup than the isolates from the USA. Analyses of the partial G gene of these European isolates clustered them in the M genogroup close to the root while the Russian isolate clustered in the U genogroup. The European isolates together with US-WRAC and US-Col-80 were also tested in an enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies (MAbs) against the N protein. MAbs 136-1 and 136-3 reacted equally at all concentrations with the isolates tested, indicating that these antibodies identify a common epitope. MAb 34D3 separated the M and L genogroup isolates from the U genogroup isolate. MAb 1DW14D divided the European isolates into 2 groups. MAb 1DW14D reacted more strongly with DE-DF 13/98 11621 and RU-FR1 than with IT-217A, FR-32/87, DE-DF 4/99-8/99 and AU-9695338. In the phylogenetic studies, the Italian, French, German and Austrian isolates clustered in the M genogroup, whereas in the serological studies using MAbs, the European M genogroup isolates could not be placed in the same specific group. These results indicate that genotypic and serotypic classification do not correlate.KEY WORDS: Infectious haematopoietic necrosis virus · IHNV · Europe · Nucleocapsid protein · Glycoprotein · Phylogeny · Enzyme-linked immunosorbent assay · ELISA Resale or republication not permitted without written consent of the publisherDis Aquat Org 86: [213][214][215][216][217][218][219][220][221] 2009 phosphoprotein (P), matrix protein (M), glycoprotein (G) and the polymerase (L); and one non-virion (NV) protein (Fauquet et al. 2005).IHNV was first observed in cultured sockeye salmon on the west coast of North America (Bootland & Leong 1999). Currently IHNV in North America is found from Alaska to northern California along the Pacific coast and inland to Idaho (Kurath et al. 2003). In Asia IHNV has been isolated from fry and fingerlings of Pacific salmon Oncorhynchus spp. and rainbow trout in Japan (Nishizawa et al. 2006) and from juvenile rainbow trout and masu salmon Oncorhynchus masou in Korea (Kim et al. 2007). In 1987 IHNV was isolated from rainbow trout yolk sac fry in Italy (Bovo et al. 1987) and from rainbow trout fry and fingerlings in France (Laurencin 1987). Since then isolation of IHNV in Europe has been reported from Austria (Kolodziejek et al. 2008), Germany (Fichtner et al. 2000, Enzmann et al. 2005, Belgium (World Organisation for Animal Health 2007) and Switzerland (Knuesel et al. 2003). The first outbreak of IHN in...

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Section: Discussionsupporting

confidence: 52%

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

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confidence: 99%

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Genetic and serological typing of European infectious haematopoietic necrosis virus (IHNV) isolates

Johansson¹,

Einer-Jensen²,

Batts³

et al. 2009

Dis. Aquat. Org.

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“…Through age calculations on six genes, the first bifurcation event of the isolates they analyzed was estimated to have occurred within the last 300 years. Additionally, comprehensive phylogenetic analyses have been performed by comparing the corresponding gene sequences of worldwide VHSV or IHNV isolates [114,119,120].…”

Section: Rhabdovirus Genomesmentioning

confidence: 99%

Virus genomes and virus-host interactions in aquaculture animals

Zhang

Gui

2015

Sci. China Life Sci.

170

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Over the last 30 years, aquaculture has become the fastest growing form of agriculture production in the world, but its development has been hampered by a diverse range of pathogenic viruses. During the last decade, a large number of viruses from aquatic animals have been identified, and more than 100 viral genomes have been sequenced and genetically characterized. These advances are leading to better understanding about antiviral mechanisms and the types of interaction occurring between aquatic viruses and their hosts. Here, based on our research experience of more than 20 years, we review the wealth of genetic and genomic information from studies on a diverse range of aquatic viruses, including iridoviruses, herpesviruses, reoviruses, and rhabdoviruses, and outline some major advances in our understanding of virus-host interactions in animals used in aquaculture.

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“…The infectious hematopoietic necrosis virus (IHNV) is one of the most important rhabdoviral fish pathogens of both wild and cultured salmon and trout and causes extensive economic losses in fish culture facilities (1,16,49). The first reported IHNV epidemics occurred during the 1950s in the United States at fish hatcheries in Washington and Oregon (20,40).…”

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confidence: 99%

Clathrin-Mediated Endocytosis in Living Host Cells Visualized through Quantum Dot Labeling of Infectious Hematopoietic Necrosis Virus

Liu

et al. 2011

J Virol

100

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Infectious hematopoietic necrosis virus (IHNV)is an important fish pathogen that infects both wild and cultured salmonids. As a species of the genus Novirhabdovirus, IHNV is a valuable model system for exploring the host entry mechanisms of rhabdoviruses. In this study, quantum dots (QDs) were used as fluorescent labels for sensitive, long-term tracking of IHNV entry. Using live-cell fluorescence microscopy, we found that IHNV is internalized through clathrin-coated pits after the virus binds to host cell membranes. Pretreatment of host cells with chlorpromazine, a drug that blocks clathrin-mediated endocytosis, and clathrin light chain (LCa) depletion using RNA interference both resulted in a marked reduction in viral entry. We also visualized transport of the virus via the cytoskeleton (i.e., actin filaments and microtubules) in real time. Actin polymerization is involved in the transport of endocytic vesicles into the cytosol, whereas microtubules are required for the trafficking of clathrin-coated vesicles to early endosomes, late endosomes, and lysosomes. Disrupting the host cell cytoskeleton with cytochalasin D or nocodazole significantly impaired IHNV infectivity. Furthermore, infection was significantly affected by pretreating the host cells with bafilomycin A1, a compound that inhibits the acidification of endosomes and lysosomes. Strong colocalizations of IHNV with endosomes indicated that the virus is internalized into these membrane-bound compartments. This is the first report in which QD labeling is used to visualize the dynamic interactions between viruses and endocytic structures; the results presented demonstrate that IHNV enters host cells via clathrin-mediated endocytic, cytoskeleton-dependent, and low-pH-dependent pathways.

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“Mid-G” Region Sequences of the Glycoprotein Gene of Austrian Infectious Hematopoietic Necrosis Virus Isolates Form Two Lineages within European Isolates and Are Distinct from American and Asian Lineages

Abstract: Infectious hematopoietic necrosis virus (IHNV) is

Cited by 15 publications

References 38 publications

Genetic and serological typing of European infectious haematopoietic necrosis virus (IHNV) isolates

Genetic and serological typing of European infectious haematopoietic necrosis virus (IHNV) isolates

Virus genomes and virus-host interactions in aquaculture animals

Clathrin-Mediated Endocytosis in Living Host Cells Visualized through Quantum Dot Labeling of Infectious Hematopoietic Necrosis Virus

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