Microwave Irradiation of PC3 Cells at Constant Culture Temperature Alters the Incorporation of BODIPY into Cells and Reduction of MTT

Abstract

The present study investigated the impact of 10 W microwave irradiation at 2.45 GHz on the cell viability and membrane properties of human prostate cancer PC3 cells, while the temperature of the cell cultures was maintained at 37±2 °C through simultaneous cooling. Microwave irradiation under these conditions did not induce apoptosis, nor otherwise lead to loss of cell viability, as indicated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), Annexin–V, and propidium iodide assays. Interestingly however, incorporation of BODIPY (4,4‐difluoro‐5‐methyl‐4‐bora‐3a,4a‐diaza‐s‐indacene) 3‐dodecanoic acid into the cell membrane was facilitated by microwave treatment at constant temperature. Also, constant temperature microwave treatment significantly increased the rate of reduction of MTT. Taken together, these results suggest that constant temperature microwave, while non‐lethal to PC3 cells, may alter membrane properties.

Microwave-assisted delivery of an anticancer drug to cancer cells

“…S1 † for representative temperature and microwave power proles for cultures in the microwave reactor). As demonstrated previously, [3][4][5] we were able to maintain the temperature of the culture within AE1-2 C of setpoint values.…”

“…3,4 Low power microwaves also appear to slightly perturb membrane properties in PC-3 prostate cancer cells, without causing apoptosis or necrosis. 5 Escherichia coli growth is also affected by exposure to non-lethal microwaves, though the effect is transient (unpublished results). Taken together, our ndings suggest that exposure of cells to low power microwave irradiation at constant temperatures causes subtle perturbations to cells without toxicity.…”

Screening of Cucumber Fusarium Wilt Bio-Inhibitor: High Sporulation Trichoderma harzianum Mutant Cultured on Moso Bamboo Medium

“…Existing studies have shown that microwave irradiation can cause damage or weakening of hydrogen bonding and base stacking chemical forces of microbial DNA molecules and change the three-dimensional structure of DNA, which leads to the occurrence of mutations (Mazinani et al, 2017). It can be seen from Figure 1A that the lethal rate of T. harzianum increased with the increase of irradiation power and the length of irradiation time; when the irradiation power was 200 W, the lethal rate was less than 20% within 100 s; when the irradiation was 400 W, the lethal rate within 100 s was less than 50%.…”

Exposure to microwave irradiation at constant culture temperature slows the growth ofEscherichia coliDE3 cells, leading to modified proteomic profiles

“…47 A Welch's t-test was carried out for all protein spots across the control and treatment groups using the following parameters: p-value based on all permutations, critical p-value of 0.01, and false discovery rate from permutations to exceed no more than 5 proteins. From the resulting list of signicantly altered spots (18), any spot with a relative standard deviation (RSD) of >40% of the normalized volume in either experimental group was excluded. The remaining spots, the majority of which exhibited #30% normalised volume RSD across all replicates, were considered genuine aer again assessing quality by manual comparison to ensure the highest possible reliability.…”

“…17 We further demonstrated that exposure of PC-3 human prostate cancer cells to non-lethal microwave irradiation (10 W) at constant culture temperature (37 C) likely led to perturbation of cell membrane properties, while no necrosis nor apoptosis was induced. 18 Most recently, we showed that uptake of the anticancer compound doxorubicin by PC-3 and human breast cancer MCF-7 cells was signicantly enhanced when the cells were exposed to nonlethal microwave irradiation. 19 In the present study, we extended our investigation to probe the inuence of microwave exposure on bacterial growth while the culture temperature was maintained constant.…”