Microwave irradiated collagen tubes as a better matrix for peripheral nerve regeneration

Ahmed, M. Rafiuddin; Vairamuthu, S.; Shafiuzama, Md.; Basha, Sabiha Hayath; Jayakumar, R.

doi:10.1016/j.brainres.2005.03.022

Cited by 42 publications

(30 citation statements)

References 27 publications

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“…The presence of macrophages (Figs. 1b and 3) in the absence of Wallerian degeneration was previously shown to be reversible when the collagen conduit was crosslinked by microwave irradiation (Ahmed et al, 2005).…”

Section: Discussionmentioning

confidence: 89%

“…Specifically, a longer gap length at longer survival times (Cai et al, 2004;Zhang et al, 2005;Haastert et al, 2006) with a different cell carrier and a known amount/duration of growth factor delivery must be studied and compared with the gold standard nerve autograft (Lee et al, 2003;Ahmed et al, 2005;Bellamkonda, 2006;Keilhoff et al, 2006). Although the nerve autograft still provides the best support for nerve regeneration followed by a conduit seeded with Schwann cells (Stang et al, 2005), both differentiated and undifferentiated MSCs are more supportive than a cell-free conduit (Tohill et al, 2004;Keilhoff et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

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Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Kempton

Gonzalez

Leven

et al. 2008

The Anatomical Record

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The early events associated with axonal growth into 10-mm nerve gaps were studied histologically in the rat sciatic nerve model to determine if the outgrowth of blood vessels, Schwann cells, and axons could be enhanced. In the first two experimental groups, collagen nerve guides were filled with either saline or Matrigel. Marrow-derived mesenchymal stem cells (MSCs) were added to Matrigel in two other groups, one of which contained cells transfected with VEGF (MSC/VEGF). After 21 days, the injury site was exposed, fixed, sectioned, and volume fractions of the conduit contents were determined by point counting. The bioresorbable collagen conduits appropriately guided the axons and vessels in a longitudinal direction. The volume fraction of axons was significantly greater in the group with saline when compared with all three groups with Matrigel. This measure had a significant positive correlation with actual counts of myelinated axons. The blood vessel volume fraction in the Matrigel group decreased compared with the saline group, but was restored in the MSC/VEGF group. All Matrigel groups had comparable cellularity and showed a distribution of residual Matrigel in acellular zones. The saline group, by contrast, sustained a network of delicate fibroblastic processes that compartmentalized the nerve and its natural matrix as it became infiltrated by axons as minifascicles. In conclusion, the reduction of axonal outgrowth in the Matrigel groups, when compared with the saline group, suggests that Matrigel may impede the early regenerative process even when enriched by the addition of MSCs or VEGF-transfected cells.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Kempton

Gonzalez

Leven

et al. 2008

The Anatomical Record

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show abstract

“…In recent years, enormous efforts have been made to fabricate the synthetic nerve conduits for the repair of peripheral nerve defects, and different kinds of biomaterials have been studied, such as polyglycolic acid (PGA) [2], poly(L-lactide-coglycolide) (PLGA) [8,9], poly(3-hydroxybutyrate) (PHB) [10,11], gelatin [12][13][14], collagen [4,8,[15][16][17][18], chitosan [2,[19][20][21][22] and silk [23,24]. And it is still an active area of intense research to seek new biomaterials with more favourable properties and more flexible processing methods [24].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) conduits for peripheral nerve regeneration

et al. 2009

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“…The tubes were then removed and washed exhaustively with doubledistilled water for a period of 3 h under agitation. The tubes were cross-linked using glutaraldehyde (GTA) (0.03%) in PBS of pH 7.4 for 30 minute [15] and irradiated with microwave irradiation (MWI) (30 s each with intermittent cooling time of 2 min) [15]. Tubes were then exhaustively washed with double-distilled water and dried under sterile conditions.…”

Section: Preparation Of Collagen Tubementioning

confidence: 99%

The Effect of Chick Embryo Cerebro-Spinal Fluid in Microwave Irradiated Collagen Guide Channel on Sciatic Nerve Regeneration in Rat

Ghavami

Dolatkhah

Farjah

2016

MOJAP

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The aim of this research was to evaluate the effect of chick embryo cerebrospinal fluid (e-CSF) across the irradiated collagen guide channel on sciatic nerve regeneration in comparison with autograft. Forty adult male Sprague-Dawley rats (250-300g) were randomized into (1) collagen channel + E-CSF, (2) collagen channel + normal saline (NS), (3) autograft (AG), and (4) sham surgery groups. The left sciatic nerve was exposed and a 10mm nerve segment was cut and removed. In the collagen groups, the proximal and distal cuts ends of sciatic nerve were telescoped into the nerve guides and E-CSF or NS injected into collagen conduit. To the autograft group, the 10 mm nerve segment was turned backwards and used an autologous nerve graft. All animals were evaluated by sciatic functional index (SFI) and electrophysiology testing. The improvements in SFI since the beginning of the last evaluation in experimental groups were measured. On days 49 and 60 post-operation, the mean SFI of the collagen + E-CSF group was significantly greater than the autograft group (P< 0.05). On day 90, the mean nerve conduction velocity (NCV) of the collagen + CSF group was greater than autograft group (P< 0.05). These findings showed that RPS in collagen nerve guide channel effectively enhances nerve regeneration and promotes functional recovery in injured sciatic nerve of rats.

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Microwave irradiated collagen tubes as a better matrix for peripheral nerve regeneration

Cited by 42 publications

References 27 publications

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Evaluation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) conduits for peripheral nerve regeneration

The Effect of Chick Embryo Cerebro-Spinal Fluid in Microwave Irradiated Collagen Guide Channel on Sciatic Nerve Regeneration in Rat

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