Microwave Corneosurfametry and the Short-Duration Dansyl Chloride Extraction Test for Rating Concentrated Irritant Surfactants

Goffin, Veroniqué; Pierard, Gérald

doi:10.1159/000051585

Cited by 12 publications

(10 citation statements)

References 8 publications

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“…However, interpreting the data in terms of 'mature' and 'immature' corneocytes could be challenged. Indeed, the pattern of variations in staining closely resembles the variegation previously reported in the corneosurfametry and corneoxenometry bioassays [6,25,26] and squamometry as well [27,28]. All these methods basically rely on the relative access of dyes to particular binding sites on corneocytes after damaging them.…”

supporting

confidence: 76%

Skin Weathering: The Face at the Interface

Pierard

2003

Dermatology

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supporting

confidence: 76%

Skin Weathering: The Face at the Interface

Pierard

2003

Dermatology

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“…The ancillary CSM and its variant microwave CSM are predictive bioassays that have shown their relevance in various experiment studies (11, 13–19, 21,22). These are inexpensive and rapid procedures allowing simultaneous comparisons of many products or concentrations, up to 2 dozen, in a couple of hours.…”

Section: Discussionmentioning

confidence: 99%

Responsive corneosurfametry following in vivo skin preconditioning

2003

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Skin is subjected to many environmental threats, some of which altering the structure and function of the stratum corneum. Among them, surfactants are recognized factors that may influence irritant contact dermatitis. The present study was conducted to compare the variations in skin capacitance and corneosurfametry (CSM) reactivity before and after skin exposure to repeated subclinical injuries by 2 hand dishwashing liquids. A forearm immersion test was performed on 30 healthy volunteers. 2 daily soak sessions were performed for 5 days. At inclusion and the day following the last soak session, skin capacitance was measured and cyanoacrylate skin-surface strippings were harvested. The latter specimens were used for the ex vivo microwave CSM. Both types of assessments clearly differentiated the 2 hand dishwashing liquids. The forearm immersion test allowed the discriminant sensitivity of CSM to increase. Intact skin capacitance did not predict CSM data. By contrast, a significant correlation was found between the post-test conductance and the corresponding CSM data. In conclusion, a forearm immersion test under realistic conditions can discriminate the irritation potential between surfactant-based products by measuring skin conductance and performing CSM. In vivo skin preconditioning by surfactants increases CSM sensitivity to the same surfactants.

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“…Microwave CSM is a more rapid procedure than the regular CSM 46. In this procedure, CSSS are immersed in a flask containing the test surfactant solution, and they are placed in a microwave oven containing a 500 mL water load.…”

Section: Analytical Morphology Of Skin Strippingsmentioning

confidence: 99%

From observational to analytical morphology of the stratum corneum: progress avoiding hazardous animal and human testings

Pierard¹,

Courtois²,

Ritacco³

et al. 2015

CCID

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BackgroundIn cosmetic science, noninvasive sampling of the upper part of the stratum corneum is conveniently performed using strippings with adhesive-coated discs (SACD) and cyanoacrylate skin surface strippings (CSSSs).MethodsUnder controlled conditions, it is possible to scrutinize SACD and CSSS with objectivity using appropriate methods of analytical morphology. These procedures apply to a series of clinical conditions including xerosis grading, comedometry, corneodynamics, corneomelametry, corneosurfametry, corneoxenometry, and dandruff assessment.ResultsWith any of the analytical evaluations, SACD and CSSS provide specific salient information that is useful in the field of cosmetology. In particular, both methods appear valuable and complementary in assessing the human skin compatibility of personal skincare products.ConclusionA set of quantitative analytical methods applicable to the minimally invasive and low-cost SACD and CSSS procedures allow for a sound assessment of cosmetic effects on the stratum corneum. Under regular conditions, both methods are painless and do not induce adverse events. Globally, CSSS appears more precise and informative than the regular SACD stripping.

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Microwave Corneosurfametry and the Short-Duration Dansyl Chloride Extraction Test for Rating Concentrated Irritant Surfactants

Cited by 12 publications

References 8 publications

Skin Weathering: The Face at the Interface

Skin Weathering: The Face at the Interface

Responsive corneosurfametry following in vivo skin preconditioning

From observational to analytical morphology of the stratum corneum: progress avoiding hazardous animal and human testings

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