Microwave Assisted Extraction and Pressurized Liquid Extraction of Sulfated Polysaccharides from Fucus virsoides and Cystoseira barbata

Dobrinčić, Ana; Pedišić, Sandra; Zorić, Zoran; Jurin, Mislav; Roje, Marin; Čož‐Rakovac, Rozelindra; Dragović–Uzelac, Verica

doi:10.3390/foods10071481

Cited by 23 publications

(10 citation statements)

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“…The sulfate content had a significant (P < 0.01) strong positive correlation with the DPPH values; r=0.94 (R 2 =89.4), in accordance with Dobrincic et al [59] . Sulfated polysaccharide may effectively donate an electron to DPPH free radicals, although the antioxidant properties of sulfated polysaccharides may be related to their hydroxyl groups.…”

Section: Chemical Composition Of Purified Sps and Their Antioxidation...supporting

confidence: 89%

Characteristics and Antioxidant Potential of Sulfated Polysaccharides from Cladophora crispata

2022

JJC

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Cladophora crispata, a diffusive green freshwater algum in Syria, forms a rich source of nutrients, especially sulfated polysaccharides (SPs). The SPs were extracted from Cladophora crispata by ultrasonic-assisted extraction using different types of solvents (methanol-70%, ethanol-70% and acetone-70%) at various temperatures (40, 60 and 80 °C) and for different extraction times (30, 75 and 120 min). Maximum yield of SPs (13%) and highest total soluble carbohydrate (74.36%) were achieved using acetone-70% as a solvent at 40 °C for 120 min. Purification of crude SPs was performed using Sephadex gel G-100. The purification process afforded two fractions (F1 and F2) for all treatments. The monosaccharides for the crude and for the F1 and F2 fractions were identified by thin-layer chromatography to consist of rhamnose, galactose, xylose and ribose. The chemical composition and antioxidant activity (DPPH and ATBS assays) of the crude and fractions were determined. FT-IR analysis revealed that the fractions consist of sugar units, protein units, sulfate groups and uronic acids, the content of which was determined following standard methods. Highest sulfate content was obtained upon extraction at low temperature (40 oC). The sulfate content had a significant strong positive correlation with the DPPH scavenging activity, but a weak negative one with the ABTS values. On the other hand, the uronic-acid content had a significant negative correlation with the DPPH values and a positive one with the ATBS values. Highest scavenging activity on DPPH radical (35.82%) was observed for the F2 fraction from the acetone-70% extract which had the highest sulfate content, while the highest scavenging effect on ABTS (39.71%) was found for the F1 fraction from the ethanol-70% extract which had the highest uronic-acid content.

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Section: Chemical Composition Of Purified Sps and Their Antioxidation...supporting

confidence: 89%

Characteristics and Antioxidant Potential of Sulfated Polysaccharides from Cladophora crispata

2022

JJC

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“…Table 2 demonstrates that there were six portions for the Mw of SHF: 3374.86 kDa (7.68%), 1166.48 kDa (44.06%), 152.37 kDa (14.50%), 121.93 kDa (13.43%), 44.94 kDa (5.91%), and 22.40 kDa (14.42%). Mn represents the statistical average molecular weight of a polymer, which is affected more by lower molecular weight chains [ 31 ]. Mp represents the molecular weight distribution mode [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Chemical Characterization and Immunomodulatory Activity of Fucoidan from Sargassum hemiphyllum

Zhou

Chen

et al. 2022

Marine Drugs

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Fucoidan is a sulfated algal polyanionic polysaccharide that possesses many biological activities. In this paper, a fucoidan (SHF) polysaccharide was extracted from Sargassum hemiphyllum collected in the South China Sea. The SHF, with a molecular weight of 1166.48 kDa (44.06%, w/w), consisted of glucose (32.68%, w/w), galactose (24.81%, w/w), fucose (20.75%, w/w), xylose (6.98%, w/w), mannose (2.76%, w/w), other neutral monosaccharides, and three uronic acids, including glucuronic acid (5.39%, w/w), mannuronic acid (1.76%, w/w), and guronuronic acid (1.76%, w/w). The SHF exhibited excellent immunostimulatory activity. An immunostimulating assay showed that SHF could significantly increase NO secretion in macrophage RAW 264.7 cells via upregulation of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) levels based on both gene expression and protein abundance. These results suggest that SHF isolated from Sargassum hemiphyllum has great potential to act as a health-boosting ingredient in the pharmaceutical and functional-food fields.

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“…To the best of our knowledge, MAE has been applied for fucoidans extraction from small number of brown seaweeds, as in case of Fucus vesiculosus [ 33 ], Fucus virsoides and Cystoseira barbata [ 37 ], and Ascophyllum nodosum [ 38 ]. However, it has not been reported for F. spiralis and L. saccharina , which have been extracted by classical solvent extraction containing CaCl 2 followed by ethanol precipitation [ 8 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown Seaweeds

et al. 2023

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Microwave-assisted extraction (MAE) is recognized as a green method for extraction of natural products. The current research aimed to explore the MAE for fucoidans extraction from different brown seaweeds, including Fucus vesiculosus, F. spiralis, and Laminaria saccharina. Following several solvent-extraction pre-treatment steps and MAE optimization, the algal biomasses were extracted in a ratio of 1:25 in 0.1 M HCl containing 2 M CaCl2 for 1.0 min. The results showed that L. saccharina’s extract was different from the others, regarding the highest sugar content reached 0.47 mg glucose equivalent/mg extract being confirmed by monosaccharide composition analysis and the lowest fucoidan content and sulfation degree at 0.09 mg/mg extract and 0.13, respectively. Moreover, these findings were confirmed by tentative structural elucidation based on Fourier-transform infrared spectrometry which also showed a different spectrum. However, the MAE enhanced melanoidins formation in products, which was confirmed by the intense band at 1420 cm−1. Interestingly, the results of monomeric composition showed that fucoidan extract by MAE from F. vesiculosus belonged to sulfated galactofucans which are known for their potential bioactivities. Furthermore, the cytotoxic activity of the four fucoidans in concentrations ranging from 4.9 µg/mL to 2500 µg/mL was investigated and correlated with the chemical characterization showing that F. vesiculosus_MAE fucoidan was the most potent and safest. The current research revealed the chemical heterogeneity of fucoidans regarding taxonomical class and used greener extraction method of fucoidans toward the achievement of the UN sustainability goals.

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Microwave Assisted Extraction and Pressurized Liquid Extraction of Sulfated Polysaccharides from Fucus virsoides and Cystoseira barbata

Cited by 23 publications

References 53 publications

Characteristics and Antioxidant Potential of Sulfated Polysaccharides from Cladophora crispata

Characteristics and Antioxidant Potential of Sulfated Polysaccharides from Cladophora crispata

Chemical Characterization and Immunomodulatory Activity of Fucoidan from Sargassum hemiphyllum

Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown Seaweeds

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