2007
DOI: 10.1016/j.idairyj.2006.05.007
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Microwave-assisted digestion of β-lactoglobulin by pronase, α-chymotrypsin and pepsin

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Cited by 23 publications
(15 citation statements)
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“…Our finding of the enhancement of the enzymatic proteolysis under MWI is in good agreement with data previously described by our group for kinetic parameters for Pronase and a-Chymotrypsin hydrolysis of bovine b-Lg (Izquierdo, Alli, Yaylayan, & Gomez, 2007). Higher catalytic effectiveness (K cat K m À1 ) values were obtained in both enzymatic digestions of the protein performed under MWI in comparison with the values in the respective CH digestions.…”
Section: Proteolysis Of Bovine Whey Protein Concentratesupporting
confidence: 92%
See 1 more Smart Citation
“…Our finding of the enhancement of the enzymatic proteolysis under MWI is in good agreement with data previously described by our group for kinetic parameters for Pronase and a-Chymotrypsin hydrolysis of bovine b-Lg (Izquierdo, Alli, Yaylayan, & Gomez, 2007). Higher catalytic effectiveness (K cat K m À1 ) values were obtained in both enzymatic digestions of the protein performed under MWI in comparison with the values in the respective CH digestions.…”
Section: Proteolysis Of Bovine Whey Protein Concentratesupporting
confidence: 92%
“…Higher catalytic effectiveness (K cat K m À1 ) values were obtained in both enzymatic digestions of the protein performed under MWI in comparison with the values in the respective CH digestions. The Michaelis-Menten constant (K m ) for either enzyme was reduced under MWI, which suggests the highest substrate-enzyme affinity at these conditions (Izquierdo et al, 2007). Furthermore, Pramanik et al (2002) reported acceleration of enzymatic processes for protein mapping by trypsin digestion.…”
Section: Proteolysis Of Bovine Whey Protein Concentratementioning
confidence: 98%
“…This result can be explained by the possible exposure of cleavage sites resulting from thermal denaturation and the increase in the susceptibility of protein to proteolysis. In addition, application of microwaves combined with enzymatic hydrolysis has been reported in several studies (El Mecherfi et al 2011; Izquierdo et al 2007, 2008). El Mecherfi et al (2011) investigated the effect of combined microwave and enzymatic hydrolysis on the human immunoglobulin E (IgE)-binding properties of β-lactoglobulin and bovine whey proteins.…”
Section: Enzymatic Hydrolysismentioning
confidence: 97%
“…As an example, these effects are used in biocatalysis discipline (Réjasse et al, 2004;2006;Yadav & Borkar, 2009;Souza et al, 2009), DNA hybridization (Edwards, Young, & Deiters, 2009) and the studies that evaluate enzyme stability with the use of microwave irradiation (Rejasse et al, 2007;Young et al, 2008;Izquierdo et al, 2007;Porcelli et al, 1997;La Cara et al, 1999). Recently, Laurence et al (2000), H. Bohr and J. Bohr (2000), Pomerai et al (2003) reported unfolding or structural changes in proteins with microwave irradiations and suggested the inclusion of www.ccsenet.org/ijc…”
Section: Applications In Proteomicsmentioning
confidence: 99%