Microvessel scaffold with circular microchannels by photoresist melting

Abstract

In this research, a new process that integrates the photoresist melting and soft lithography techniques to fabricate microvessel scaffolds with circular microchannels is proposed. The commercial software COMSOL Multiphysics (formerly known as FEMLAB) is the sought after procedure to optimize the structure of the microvessel scaffold. The photolithographic technique is applied to fabricate the negative photoresist THB-120N (JSR Inc.) based microstructure that is followed by melting to the final replica mold with its structure having convex semicircle cross-section. The replica mold is hence used to replicate PDMS to the top and bottom plates of a microvessel scaffold. These two half plates are bonded after having surface treatment by inductive coupled plasma (ICP) to form the complete scaffold with circular microchannels. Finally, the bovine endothelial cells (BEC) are cultured into the scaffold. Encouraging results by semi-dynamic seeding have been observed in this context, depicting the survival of the cells in the scaffold for up to 4 weeks.

Fabrication and experiment of a hydrophilic micromixer using optically clear adhesive tape

“…In Table 1, we list and calculate the mixing efficiency of the OCA tape-glass micromixer in which the mixing efficiency at entrance of baffled structure is 21 % while at exit of the baffled structure is obtained at 71 %. We can conclude that the mixing occurred in the type of 90 × 150 m baffled tape-glass microchannel is acceptable due to its easy fabrication method and time-saving, efficient manufacturing process, compared to those of PMMA chips (Bubendorfer et al 2007;Zimmermann et al 2008;Wang et al 2006Wang et al , 2007 and PDMS chips (Bhagat et al 2007;Chen et al 2006). In part, this is because smaller baffle spaces will increase the interfacial contact areas and decrease the diffusive distances in length.…”

“…In addition, a report using the negative photoresist JSR, lithography and PMMA polymer as a substrate is being employed in Wang et al (2006) for the formation of microchannel structures. In recent years, an approach that integrates the melting of photoresist and soft lithography technique was proposed to fabricate the microvessel scaffolds with circular microchannels (Wang et al 2007).…”

Fabrication of Pillared PLGA Microvessel Scaffold Using Femtosecond Laser Ablation

“…To solve the dead volume problem, the same group developed microvessel scaffolds with circular microchannels on both polydimethylsiloxane and PLGA substrates using the photoresist melting approach. 11,12 Borenstein et al have recently reported on the fabrication of another circular cross-sectional microvascular scaffold structure based on the polystyrene substrate. Successful seeding of primary human umbilical vein endothelial cells was observed.…”

Simple and low-cost fabrication of PDMS microfluidic round channels by surface-wetting parameters optimization

“…A conventional way is photolithography: Futai et al (2004) used a backside diffused light photography strategy combined with small aperture sizes on their photomask to produce 200 lm wide semiround channels. Wang et al (2007) have fabricated 500 lm wide and 70°TCA channels using a negative photoresist (THB 120N from JSR Inc.) with a reflow after development. A positive photoresist (AZ9260, Hoechst) combined to a plasma polymerization technique of tetramethyldisiloxane (TMDS) was employed to create 25 lm wide straight rounded channels (Abbas et al 2009).…”