Microvessel quantification in primary colorectal carcinoma: an immunohistochemical study

Vermeulen, PB; Verhoeven, Didier; Fierens, H.; Hubens, Guy; Goovaerts, G; Marck, E. Van; Bruijn, Ernst de; Oosterom, A. Van; Dirix, L.

doi:10.1038/bjc.1995.68

Cited by 71 publications

(46 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For patients with and without invasive disease, vWF + vessels were at lower density in DCIS than in the normal breast. This is in keeping with observations on invasive tumours (colorectal carcinoma) where vWF immunostaining has been found to be absent from some of the capillaries in the tumour (Vermeulen et al, 1995). Anti-vWF stains large vessels more strongly than small ones (Vermeulen et al, 1996) and consequently our findings could reflect the immaturity of newly formed tumour-associated vessels.…”

Section: Mvd (Vessels MMsupporting

confidence: 92%

Vascular density and phenotype around ductal carcinoma in situ (DCIS) of the breast

et al. 2002

View full text Add to dashboard Cite

Up to 50% of recurrences of ductal carcinoma in situ of the breast are associated with invasive carcinoma but no pathological or molecular features have yet been found to predict for the development of invasive disease. For a tumour to invade, it requires the formation of new blood vessels. Previous studies have described a vascular rim around ducts involved by ductal carcinoma in situ, raising the possibility that the characteristics of periductal vascularisation may be important in determining transformation from in situ to invasive disease. Periductal vascular density and phenotype were determined using morphometry and a panel of anti-endothelial antibodies (von Willebrand factor, CD31, CD141 and CD34) and related to the presence of invasive carcinoma and other histological features. Compared to normal lobules, pure ductal carcinoma in situ exhibited a greater density of CD34 + and CD31 + vessels but a decrease in those that were immunopositive for vWF, indicating a difference in phenotype and in density. Ductal carcinoma in situ associated with invasive carcinoma showed a profile of vascular immunostaining similar to that of pure ductal carcinoma in situ but there were significantly greater numbers of CD34 + and CD141 + vessels and fewer staining for vWF. There was a significant negative correlation between vascular density and both the cross-sectional areas of the ducts involved and the extent of the necrosis of the tumour they contained. A correlation between vascular density and nuclear grade was also noted, being highest in the intermediate grade. The greater density of CD34 + and CD141 + vessels around ductal carcinoma in situ associated with invasive carcinoma could reflect a greater predisposition to invade but a direct effect of co-existent invasive carcinoma cannot entirely be ruled out in the present study. The relationship between vascular density, grade, duct size and nuclear grade suggests that periductal angiogenesis increases with tumour growth rate but is unable to keep pace with the most rapidly growing lesions.

show abstract

Section: Mvd (Vessels MMsupporting

confidence: 92%

Vascular density and phenotype around ductal carcinoma in situ (DCIS) of the breast

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Staining for CD3 and CD20 was performed as well. We chose CD34 as endothelial marker as it does not cross-react with lymphoid cells on frozen tissue, as CD31, and stains a wider range of intratumour vessels than anti-Factor VIII antibodies (Vermeulen et al, 1995).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Vascular patterns in reactive lymphoid tissue and in non-Hodgkin's lymphoma

et al. 2003

View full text Add to dashboard Cite

The few studies published on angiogenesis in lymphoma have raised the question of whether or not microvessel density (MVD) is associated with more aggressive disease and have reported the observation that in follicular lymphomas, vessels are mature rather than immature. We investigated MVD and the vascular phenotype within follicular or diffuse large B-cell lymphomas, reactive nodes and tonsils. Vascular phenotype was defined by the expression or loss of reactivity to the antibody LH39 (detecting the LH39 laminin epitope of the basement membrane in mature vessels) and by detection of aVb3 (expressed on immature vessels). In reactive nodes and in follicular lymphomas, MVD was higher in the paracortex than in germinal centres or in neoplastic follicles. However, in neoplastic follicles an increase in aVb3-positive endothelium suggested the activation of an angiogenic pathway different from that present in the reactive follicles. In large B-cell lymphomas, MVD was higher than in reactive and neoplastic follicles but lower than in the reactive paracortex. The number of immature vessels (LH39 negative) and of aVb3-positive vessels was higher than in reactive lymph nodes and follicular lymphoma suggesting that a switch to a different angiogenic pathway has occurred. Finally, we have demonstrated that within reactive and neoplastic follicles vascular regression is occurring, perhaps constraining the growth of reactive follicles alongside other phenomena such as apoptosis. Vascular regression was previously believed to occur in adults only in ovarian and endometrial tissue. We conclude that different types of angiogenesis are present in follicular lymphomas and large B-cell lymphomas. This has implications for possible future therapies.

show abstract

“…14,17 Selection of hot spot for the study of angiogenesis has been adopted as Leukemia a standard procedure in solid neoplasms, but also in some hematological oncology studies. 14,23,34 It is also worthy of note, that according to Padro et al 14 and our findings, microvessel counting using hot spots is truly representative of the total degree of angiogenesis in the bone marrow samples.…”

Section: Figurementioning

confidence: 99%

Prognostic evaluation of the microvascular network in myelodysplastic syndromes

et al. 2001

View full text Add to dashboard Cite

Considering the recently stated suggestion of neovascularization being implicated in myelodysplastic syndromes (MDS) pathogenesis, we evaluated multiple morphometric microvascular characteristics in MDS, in relation to clinicopathologic factors and prognosis. Trephines from 50 newly diagnosed MDS patients were immunostained for factor VIII and compared to those from 20 controls, 10 chronic myelomonocytic leukemia (CMML) and 12 acute myeloid leukemia (AML) patients. Quantitation of microvessel density (MVD), area, total vascular area (TVA), major and minor axis length, perimeter, compactness, shape factor, Feret diameter, and the number of branching vessels was performed by image analysis. Overall, the MDS group had significantly higher MVD, TVA, minor axis and shape factor values and significantly lower compactness than the control group. AML was characterized by increased vascularity compared to MDS and CMML, as well as by the presence of flattened microvessels (lower values of shape factor). Hypercellular MDS showed higher MVD. RA/RARS displayed larger caliber vessels than RAEB, which explains the favorable prognostic effect of increased size-related parameters on progression and/or survival. Moreover, decreased compactness and MVD were independent predictors of longer progression-free survival. It is concluded that angiogenesis is involved in the conversion of normal marrow to MDS and ultimately to AML and that disease progression within MDS is accompanied by qualitative alterations of the microvascular network. Furthermore, size-related parameters affect survival, while shape-related parameters and MVD are more influential with regard to progression-free survival. Leukemia (2001Leukemia ( ) 15, 1369Leukemia ( -1376

show abstract

Microvessel quantification in primary colorectal carcinoma: an immunohistochemical study

Cited by 71 publications

References 11 publications

Vascular density and phenotype around ductal carcinoma in situ (DCIS) of the breast

Vascular density and phenotype around ductal carcinoma in situ (DCIS) of the breast

Vascular patterns in reactive lymphoid tissue and in non-Hodgkin's lymphoma

Prognostic evaluation of the microvascular network in myelodysplastic syndromes

Contact Info

Product

Resources

About