Microvascular hemodynamics in experimental arthritis: Disparity between the distribution of microspheres and plasma flow in bone

Hansen, Ebbe Stender; Søballe, Kjeld; Kjølseth, Dorthe; Henriksen, Tine Brink; He, Shu-Zheng

doi:10.1016/0026-2862(90)90020-r

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…Although 15-pm MSs were evenly distributed between the brachial artery and aorta, one could suspect skimming (Yen and Fung 1978) to influence the distribution of even these small microspheres at the microcirculatory level as suggested by in vitro (@fjord et al 1981,Ibfjord andClausen 1983) and in vivo observations in the heart (Bassingthwaighte et al 1988) and in bone (Hansen et al 1990a). Comparison between deposition of microspheres and the novel plasma soluble flow marker 2-iododesmethylimipramine (the so-called molecular microsphere; Bassingthwaighte 1983, Little et al 1986) may cast light on this problem in the future.…”

Section: Discussionmentioning

confidence: 99%

Arteriovenous shunting is not associated with venous congestion in bone: Knee tamponade studied with 15-μm and 50-μm microspheres in immature dogs

Hansen

Hjortdal

KjøSlseth

et al. 1991

Acta Orthopaedica Scandinavica

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Section: Discussionmentioning

confidence: 99%

Arteriovenous shunting is not associated with venous congestion in bone: Knee tamponade studied with 15-μm and 50-μm microspheres in immature dogs

Hansen

Hjortdal

KjøSlseth

et al. 1991

Acta Orthopaedica Scandinavica

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“…7,8,19,26,30,31,39,44,48,67 Even though fluorescent microspheres have been used sporadically for bone blood flow measurement, 41,59 the quantitative analysis of the number of microspheres in the tissue sample and the precision of the microsphere technique using fluorescent or radioactive microspheres have not been established in bone. To minimize the theoretical and methodologic errors, a systematic evaluation of the microsphere method for bone blood flow measurement is necessary.…”

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confidence: 99%

Microspheres Accurately Predict Regional Bone Blood Flow

Anetzberger¹,

Thein²,

Becker³

et al. 2004

Clinical Orthopaedics &Amp; Related Research

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Even though the microsphere method frequently is used to determinate bone blood flow, validation of this technique for bone blood flow measurement is incomplete. The method is based on the principle that injected microspheres are distributed with the arterial blood and trapped in the capillaries because of their diameter (15 µm). The number of spheres lodged in an organ is proportional to its blood flow. The number of radioactive or fluorescent microspheres in a specific organ is determined indirectly by measuring radioactivity or fluorescence intensity in the organ. In this study the reliability and precision of the microsphere method for determining bone blood flow was established using radioactive and fluorescent microspheres. Six female, anesthetized New Zealand rabbits received left ventricular injections of pairs of fluorescent and/or radioactive microspheres. The humerus, femur, and tibia were dissected in a standardized manner and blood flow was determined in each sample. Comparison of relative blood flow values showed an excellent correlation between radioactive and fluorescent microspheres. The percentage difference and variation between two simultaneously injected sets of microspheres was minimal for radioactive microspheres (0.8% ± 9.6%) and for fluorescent microspheres (0.2% ± 11.4%). Regional bone blood flow in different regions of the femur, tibia, or humerus ranged from 2.2-28.1 mL/minute/100 g, but there was no significant difference between right and left bone samples of the same region after repeated measurement. Radioactive and fluorescent microspheres allow precise determination of regional bone blood flow.

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[^99mTc]Diphosphonate uptake and hemodynamics in experimental arthritis: Effect of naproxen in the canine carrageenan injection model

Hansen

Søballe

et al. 1992

Journal Orthopaedic Research

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The impact of naproxen treatment on juxta-articular hemodynamics and bone metabolism in experimental juvenile arthritis was studied in the articular carrageenan injection model. Unilateral gonarthritis was induced for 12 weeks in eight dogs receiving naproxen (dosage, 2 mg/kg) and eight controls. Regional blood flow was assessed by the microsphere method, plasma volume by the distribution space of [125I]fibrinogen, and bone metabolism by the 2-h uptake of [99mTc]diphosphonate ([99mTc]DPD). Synovial effusion was less prominent with naproxen treatment as judged by joint fluid volume and pressure. Naproxen reduced the arthritic capsular hyperemia, almost normalized a severe blood flow increase in patella and both juxta-articular epiphyses, ameliorated an expansion of plasma volume in the patella and the distal femoral epiphysis, and normalized an increased [99mTc]DPD uptake in subchondral femoral bone and the tibial cortex. Significantly increased arteriovenous shunting in the arthritic extremity was unaffected by naproxen. The study suggests that long-term cyclooxygenase inhibition offers protection against hemodynamic and metabolic changes in juxta-articular bone secondary to synovial inflammation.

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Microvascular hemodynamics in experimental arthritis: Disparity between the distribution of microspheres and plasma flow in bone

Cited by 5 publications

References 18 publications

Arteriovenous shunting is not associated with venous congestion in bone: Knee tamponade studied with 15-μm and 50-μm microspheres in immature dogs

Arteriovenous shunting is not associated with venous congestion in bone: Knee tamponade studied with 15-μm and 50-μm microspheres in immature dogs

Microspheres Accurately Predict Regional Bone Blood Flow

[^99mTc]Diphosphonate uptake and hemodynamics in experimental arthritis: Effect of naproxen in the canine carrageenan injection model

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Microvascular hemodynamics in experimental arthritis: Disparity between the distribution of microspheres and plasma flow in bone

Cited by 5 publications

References 18 publications

Arteriovenous shunting is not associated with venous congestion in bone: Knee tamponade studied with 15-μm and 50-μm microspheres in immature dogs

Arteriovenous shunting is not associated with venous congestion in bone: Knee tamponade studied with 15-μm and 50-μm microspheres in immature dogs

Microspheres Accurately Predict Regional Bone Blood Flow

[99mTc]Diphosphonate uptake and hemodynamics in experimental arthritis: Effect of naproxen in the canine carrageenan injection model

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[^99mTc]Diphosphonate uptake and hemodynamics in experimental arthritis: Effect of naproxen in the canine carrageenan injection model