Microtubule‐mediated Peroxisomal Saltations<sup>a</sup>

Rapp, S.; Saffrich, Rainer; Jäkle, Ursula; Ansorge, Wilhelm; Gorgas, Karin; Just, Wilhelm W.

doi:10.1111/j.1749-6632.1996.tb18659.x

Cited by 49 publications

(87 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rapid movement of some GLUT4 vesicles was reminiscent of the microtubule-based movements of peroxisomes in CV1 cells [21,22]. In these studies, peroxisomes were shown to move along microtubules at approx.…”

Section: Time-lapse Confocal Microscopy Of Gfp-glut4 Movementsmentioning

confidence: 93%

GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein

Oatey

Weering

Dobson

et al. 1997

Biochemical Journal

View full text Add to dashboard Cite

Insulin stimulates glucose uptake into its target cells by a process which involves the translocation of the GLUT4 isoform of glucose transporter from an intracellular vesicular compartment(s) to the plasma membrane. The step(s) at which insulin acts in the vesicle trafficking pathway (e.g. vesicle movement or fusion with the plasma membrane) is not known. We expressed a green-fluorescent protein-GLUT4 (GFP-GLUT4) chimaera in 3T3 L1 adipocytes. The chimaera was expressed in vesicles located throughout the cytoplasm and also close to the plasma membrane. Insulin promoted a substantial translocation of GFP-GLUT4 to the plasma membrane. Time-lapse confocal microscopy demonstrated that the majority of GFP-GLUT4-containing vesicles in the basal state were relatively static, as if tethered (or attached) to an intracellular structure. A proportion (approx. 5%) of the vesicles spontaneously lost their tether, and were observed to move rapidly within the cell. Other vesicles appear to be tethered only on one edge and were observed in a rapid stretching motion. The data support a model in which GLUT4-containing vesicles are tightly tethered to an intracellular structure(s), and indicate that a primary site of insulin action must be to release these vesicles, allowing them to then translocate to and fuse with the plasma membrane.

show abstract

Section: Time-lapse Confocal Microscopy Of Gfp-glut4 Movementsmentioning

confidence: 93%

GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein

Oatey

Weering

Dobson

et al. 1997

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…It was unclear whether this CD-induced effect pointed to indirect or direct interaction with the actin cytoskeleton. As a consequence, Rapp et al (1996) suggested that the actin-dependent organelle motility that seemed to be coordinated with the microtubule system possibly fulfilled a subservient role in organelle transport. In view of the more recent reports of cooperative interaction between subcellular organelles and actin and microtubule motors, one is inclined to believe that peroxisome movement in animal cells may also favor a cooperative rather than an exclusive track system.…”

Section: Actin Microfilaments and Not Microtubules Are Involved In Thmentioning

confidence: 99%

“…Studies on peroxisomal motility in animal cells have demonstrated microtubules as the preferred tracks for peroxisome movement (Rapp et al, 1996;Schrader et al, 1996;Wiemer et al, 1997). Observations leading to this conclusion in chicken hamster ovary cells (Rapp et al, 1996;Huber et al, 2000) included evidence from transmission electron microscopy, which revealed a close association between peroxisomes and microtubules.…”

Section: Actin Microfilaments and Not Microtubules Are Involved In Thmentioning

confidence: 99%

“…Further evidence involved a complete cessation of saltations in response to the antimicrotubule drugs nocodazole and colcemid. However, Rapp et al (1996) conceded that the intracellular movement of peroxisomes was influenced not only by nocodazole treatment but also, to a lesser extent, by the actin polymerization inhibitor CD. Thus, saltations were still observed in CDtreated cultures, but their velocity was reduced by nearly 25%.…”

Section: Actin Microfilaments and Not Microtubules Are Involved In Thmentioning

confidence: 99%

“…Observations leading to this conclusion in chicken hamster ovary cells (Rapp et al, 1996;Huber et al, 2000) included evidence from transmission electron microscopy, which revealed a close association between peroxisomes and microtubules. Further evidence involved a complete cessation of saltations in response to the antimicrotubule drugs nocodazole and colcemid.…”

Section: Actin Microfilaments and Not Microtubules Are Involved In Thmentioning

confidence: 99%

See 2 more Smart Citations

Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants,

2002

View full text Add to dashboard Cite

Peroxisomes were visualized in living plant cells using a yellow fluorescent protein tagged with a peroxisomal targeting signal consisting of the SKL motif. Simultaneous visualization of peroxisomes and microfilaments/microtubules was accomplished in onion (Allium cepa) epidermal cells transiently expressing the yellow fluorescent protein-peroxi construct, a green fluorescent protein-mTalin construct that labels filamentous-actin filaments, and a green fluorescent proteinmicrotubule-binding domain construct that labels microtubules. The covisualization of peroxisomes and cytoskeletal elements revealed that, contrary to the reports from animal cells, peroxisomes in plants appear to associate with actin filaments and not microtubules. That peroxisome movement is actin based was shown by pharmacological studies. For this analysis we used onion epidermal cells and various cell types of Arabidopsis including trichomes, root hairs, and root cortex cells exhibiting different modes of growth. In transient onion epidermis assay and in transgenic Arabidopsis plants, an interference with the actin cytoskeleton resulted in progressive loss of saltatory movement followed by the aggregation and a complete cessation of peroxisome motility within 30 min of drug application. Microtubule depolymerization or stabilization had no effect.

show abstract

Biogenesis of peroxisomes in fetal liver

Espeel

Depreter

Nardacci

et al. 1997

Microsc. Res. Tech.

View full text Add to dashboard Cite

Microtubule‐mediated Peroxisomal Saltations^a

Cited by 49 publications

References 6 publications

GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein

GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein

Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants,

Biogenesis of peroxisomes in fetal liver

Contact Info

Product

Resources

About

Microtubule‐mediated Peroxisomal Saltationsa

Cited by 49 publications

References 6 publications

GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein

GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein

Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants,

Biogenesis of peroxisomes in fetal liver

Contact Info

Product

Resources

About

Microtubule‐mediated Peroxisomal Saltations^a