Microtomography on the ANATOMIX beamline at Synchrotron SOLEIL

Weitkamp, Timm; Scheel, Mario; Perrin, Jonathan; Daniel, Guillaume; King, Andrew; Roux, Vincent Le; Giorgetta, JL; Carcy, Alexandre; Langlois, Florent; Desjardins, Kewin; Menneglier, Claude; Cerato, Matthieu; Engblom, Christer; Cauchon, G.; Moreno, Thierry; Rivard, Camille; Gohon, Yann; Polack, F.

doi:10.1088/1742-6596/2380/1/012122

Cited by 19 publications

(10 citation statements)

References 13 publications

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“…Following overnight fixation, the specimens were placed in 2 mL screw-top tubes filled with PBS containing 100 U/mL penicillin and 1 μg/mL streptomycin (both Gibco). The specimens were scanned using Synchrotron radiation microcomputed tomography (SR-μCT) at the Anatomix beamline at the Synchrotron SOLEIL; the beamline and its instrumentation are described elsewhere. , In brief, the voxel size was 3.07 μm 3 , and the central energy of the filtered white X-ray beam was around 40 keV. Each scan involved a slight offset of the rotation axis in relation to the detector field of view, aiming to enlarge the diameter of the reconstructed volume.…”

Section: Methodsmentioning

confidence: 99%

Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection

Tölken,

Wassilew,

Grolimund

et al. 2023

ACS Biomater. Sci. Eng.

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Cobalt−chromium−molybdenum (CoCrMo) alloys are routinely used in arthroplasty. CoCrMo wear particles and ions derived from arthroplasty implants lead to macrophage-driven adverse local tissue reactions, which have been linked to an increased risk of periprosthetic joint infection after revision arthroplasty. While metal-induced cytotoxicity is well characterized in human macrophages, direct effects on their functionality have remained elusive. Synchrotron radiation X-ray microtomography and X-ray fluorescence mapping indicated that peri-implant tissues harvested during aseptic revision of different arthroplasty implants are exposed to Co and Cr in situ. Confocal laser scanning microscopy revealed that macrophage influx is predominant in patient tissue. While in vitro exposure to Cr 3+ had only minor effects on monocytes/macrophage phenotype, pathologic concentrations of Co 2+ significantly impaired both, monocyte/macrophage phenotype and functionality. High concentrations of Co 2+ led to a shift in macrophage subsets and loss of surface markers, including CD14 and CD16. Both Co 2+ and Cr 3+ impaired macrophage responses to Staphylococcus aureus infection, and particularly, Co 2+exposed macrophages showed decreased phagocytic activity. These findings demonstrate the immunosuppressive effects of locally elevated metal ions on the innate immune response and support further investigations, including studies exploring whether Co 2+ and Cr 3+ or CoCrMo alloys per se expose the patients to a higher risk of infections post-revision arthroplasty.

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Section: Methodsmentioning

confidence: 99%

Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection

Tölken,

Wassilew,

Grolimund

et al. 2023

ACS Biomater. Sci. Eng.

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“…Formaldehyde-fixed, ethanol-dehydrated samples were imaged with X-ray phase-contrast tomography (XPCT) at the ANATOMIX beamline [28] with a pink beam of mean energy 22 keV and a detector pixel size of 6.5 µm. The detector system consisted of a lutetium aluminum garnet (LuAG, Lu 3 Al 5 O 12 ) single-crystal scintillator (600 µm thick) coupled to a CMOS camera (Hamamatsu Orca Flash 4.0 V2, 2048 × 2048 pixels) via a 1:1 lens system consisting of two Hasselblad photo objectives ( f = 100 mm) in tandem geometry.…”

Section: Methodsmentioning

confidence: 99%

Section: X-ray Phase-contrast Tomography (Xpct)mentioning

confidence: 99%

Why are amyloid-β plaques detected by X-ray phase-contrast imaging? Role of metals revealed through combined synchrotron infrared and X-ray fluorescence microscopies

Chourrout

Sandt

Weitkamp

et al. 2022

Preprint

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Amyloid-β (Aβ) plaques from Alzheimer's Disease (AD) can be visualized ex vivo in label-free brain samples using synchrotron X-ray phase-contrast tomography (XPCT). However, for XPCT to be useful as a screening method for amyloid pathology, it is mandatory to understand which factors drive the detection of Aβ plaques. The current study was designed to test the hypothesis that the Aβ-related contrast in XPCT could be caused by the Aβ fibrils and/or by metal entrapment within the Aβ plaques. This study probed the fibrillar and elemental compositions of Aβ plaques in brain samples from different types of AD patients and different AD animal models to establish a relationship between XPCT contrast and Aβ plaque characteristics. XPCT, micro-Fourier-Transform Infrared (μFTIR) spectroscopy and micro-X-Ray Fluorescence (μXRF) spectroscopy were conducted under synchrotron radiation on human samples (genetic and sporadic cases) and on four transgenic rodent strains (mouse: APPPS1, ArcAβ, J20; rat: TgF344). Aβ plaques from the genetic AD patient were visible using XPCT, and had higher β-sheet content and higher metal levels than those from the sporadic AD patient, which remained undetected by XPCT. Aβ plaques in J20 mice and TgF344 rats appeared hyperintense on XPCT images, while they were hypointense in the case of APPPS1 and ArcAβ mice. In all four transgenic strains, β-sheet content was similar, while metal levels were highly variable: J20 mice (zinc and iron), and TgF344 (copper) showed greater metal accumulation than APPPS1 and ArcAβ mice. In humans, the greater and more diffuse metal accumulation led to a positive contrast in the genetic case of AD. Hence, contrast formation of Aβ plaques in XPCT images depended mostly on biometal entrapment.

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“…The X‐ray nano tomographic analysis of the calendered electrode under different compression pressures was performed at the beamline Anatomix of Synchrotron SOLEIL, France [45] where transmission X‐ray microscopy (TXM) instrument was used in absorption mode [46] . The scans were conducted with a monochromatic X‐ray beam of photon energy 8.327 keV using a TXM configuration.…”

Section: Experimental Analysismentioning

confidence: 99%

Computational Model for Predicting Particle Fracture During Electrode Calendering

Xu,

Paredes‐Goyes,

et al. 2023

Batteries & Supercaps

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In the context of calling for low carbon emissions, lithium‐ion batteries (LIBs) have been widely concerned as a power source for electric vehicles, so the fundamental science behind their manufacturing has attracted much attention in recent years. Calendering is an important step of the LIB electrode manufacturing, and associated changes in the electrode microstructure and mechanical properties are worthy of study. In this work, we report the observed cracking of active material (AM) particles due to calendering pressure under ex situ X‐ray nano tomography experiments. We developed an innovative 3D discrete element method (DEM) model with bonded connections to physically mimic the calendering process using real AM particle shapes derived from the tomography experiments. The DEM model can well predict the change of the morphology of the dry electrode under pressure, and the changes of the applied pressure and porosity are consistent with the experiments. The model is also able to simulate the secondary AM particles cracking by the fracture of the bond under force. Our model is the first of its kind that can predict the fracture of the secondary particles along the calendering process. This work provides a tool for guidance in the manufacturing of optimized LIB electrodes.

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Microtomography on the ANATOMIX beamline at Synchrotron SOLEIL

Cited by 19 publications

References 13 publications

Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection

Cobalt and Chromium Ions Impair Macrophage Response to Staphylococcus aureus Infection

Why are amyloid-β plaques detected by X-ray phase-contrast imaging? Role of metals revealed through combined synchrotron infrared and X-ray fluorescence microscopies

Computational Model for Predicting Particle Fracture During Electrode Calendering

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