Microtiter method to monitor Corynespora cassiicola and sensitivity of the pathogen to carbendazim, prothioconazole and pyraclostrobin

Xavier, Sheila Ariana; Mello, Flávia Elis de; Silva, Helen Prudente da; Canteri, Marcelo Giovanetti; Koga, Lucimara Junko; Lopes, I. de O. N.; Godoy, C. V.

doi:10.1016/j.cropro.2021.105554

Cited by 10 publications

(6 citation statements)

References 17 publications

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“…The EC 50 assay is one of the microbiological methods for the indication of the chemical agent concentration, which was applied to an in vitro culture of pathogens that reduced their density to 50% of the control produced without applying the chemical agent (Alexander et al 1999;Huber and Koella 1993). Several studies used an EC 50 assay to explore the substance e cacy of the inhibitory effect on the fungal pathogen growth (Alu et al 2020;Tunsagool, et al 2021;Xavier et al 2021). In this study, a 50% growth rate reduction of P. palmivora in vitro by the applications of the CLP extracts, fengycin, iturin A, and surfactin was conducted using the EC 50 assay.…”

Section: )mentioning

confidence: 99%

WITHDRAWN: Global metabolic changes by Bacillus cyclic lipopeptide extracts on stress responses of para rubber leaf

Tunsagool

Kruaweangmol

Sunpapao

et al. 2022

Preprint

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The changing environmental conditions generate the abiotic stress like exposing of chemicals and biotic stress like Phytophthora palmivora infection which is causes leaf fall disease affecting the growth rate of para rubber seedlings resulting in economic loss. To prevent abiotic and biotic stresses, biocontrol agents like cyclic lipopeptides (CLPs) from Bacillus spp. have been introduced to reduce the usage of chemically synthesized fungicides and fertilizers. This study aimed to use Bacillus CLP extracts as a biological agent to stimulate the plant growth system in para rubber seedlings comparing with an exogenous plant hormone (salicylic acid; SA) using untargeted metabolomics. CLP extracts were obtained from B. subtilis PTKU12 and exogenous SA that were each applied on the leaves of para rubber seedlings. The expressed metabolic network responding to stress induced by exposing of different agents was analyzed using liquid chromatography-tandem mass spectrometry. There were 1,702 and 979 metabolites which were detected in positive and negative ion modes of electrospray ionization, respectively. The application of CLP extracts showed the greatest accumulation of up-regulated metabolite changes in the leaves belonging to 56 metabolic pathways. The analysis of metabolic pathways indicated that CLP extracts played an important role in alanine, aspartate and glutamate metabolism to stress response leading to plant growth promotion. These findings revealed the effect of Bacillus CLP extracts on plant growth promotion considering as a protective option for para rubber plantation.

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Section: )mentioning

confidence: 99%

WITHDRAWN: Global metabolic changes by Bacillus cyclic lipopeptide extracts on stress responses of para rubber leaf

Tunsagool

Kruaweangmol

Sunpapao

et al. 2022

Preprint

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“…The sensitivity of isolates was determined by microtitre plate assays (Xavier et al, 2021) using different concentrations of fungicides. Stock solutions of 600 μg/ml were prepared to obtain the following final concentrations of each fungicide: 0.0125, 0.05, 0.2, 4, 20, and 100 μg/ml in yeast Bacto extract medium (YBS; yeast extract 5g/L, Bacto peptone 5 g/L, sodium acetate 10 g/L).…”

Section: Ec 50 Of C Cassiicola Isolates To Pyraclostrobin and Carbendazim Fungicidesmentioning

confidence: 99%

“…The EC 50 values of the fungicides were estimated from the fungal growth by the regression probit model (Finney, 1971) Based on the sensitivity differences of the isolates to QoI and MBC, isolates were classified as highly resistant (HR), EC 50 ≥ 50 μg/ ml; moderately resistant (MR), 1 µg/ml < EC 50 < 50 µg/ml; or sensitive (S), EC 50 ≤ 1 μg/ml (Edgington et al, 1971). In this study, the EC 50 of C. cassiicola was estimated using a microtitre assay to optimize space, time, and material consumption (Xavier et al, 2021).…”

Section: Ec 50 Of C Cassiicola Isolates To Pyraclostrobin and Carbendazim Fungicidesmentioning

confidence: 99%

Resistance ofCorynespora cassiicolafrom soybean to QoI and MBC fungicides in Brazil

et al. 2021

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Target spot on soybean (Glycine max), caused by the fungus Corynespora cassiicola, was first reported in Brazil in 1976 (Almeida et al., 1976). The disease was considered of minor importance for many years. Recently, the severity of the disease has been increasing, and it has now been reported in all Brazilian soybean-growing regions. Management strategies include seed treatment, crop rotation, the use of tolerant/resistant cultivars, and chemical control (Godoy et al., 2016). The disease has a significant effect on yield,

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“…EC 50 was described as the chemical agent concentration which was added to an in vitro culture of pathogens that decreases their density to 50% of the control produced without adding the chemical agent [ 34 ]. The EC 50 method was conducted to investigate the effect of substances on the suppression of fungal pathogens in previous studies [ 35 , 36 ]. To determine the effective dose of CLPs that caused a 50% growth rate reduction of M. canis , EC 50 assay was carried out in vitro .…”

Section: Discussionmentioning

confidence: 99%

Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats

Tunsagool

Ploypetch

Jaresitthikunchai

et al. 2021

Heliyon

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Background and aim Microsporum canis ( M. canis ) is a dermatophyte fungal pathogen that causes ringworms. Cats are considered to be a dominant reservoir host enabling M. canis transmission to humans. The concerns of dermatophyte resistance were raised among the usage of antifungal drugs to treat the ringworm. This study aimed to evaluate the fungal activity of cyclic lipopeptides (CLPs) obtained from Bacillus subtilis ( B. subtilis ) as an alternative method for the inhibition of M. canis growth. Materials and methods The culture plate of M. canis from confirmed cats with ringworm infection was provided. The purification of CLP extract, fengycin, iturin A, and surfactin was carried out from B. subtilis by preparative thin-layer chromatography (PTLC) coupled with solid-phase extraction (SPE) methods. Half-maximal effective concentration (EC 50 ) and agar well diffusion assays were performed to determine the efficacy of Bacillus CLP extract, fengycin, iturin A, and surfactin to inhibit the growth of M. canis isolated from cats. Results All purified Bacillus substances displayed antifungal activity to control the growth of M. canis when compared with 80% ethanol (control). EC 50 values for CLP extract, fengycin, iturin A, and surfactin were 0.23, 0.05, 0.17, and 0.08 mg/mL, respectively. In agar well diffusion assay, the ability of CLP extract, fengycin, iturin A, and surfactin on fungal inhibition had no statistically significant difference at 24 and 48 h after treatment (p < 0.05). However, CLP extract showed a statistically significant difference on M. canis inhibition at 62.21% followed by surfactin with 59.04% at 72 h after treatment. Conclusion In vitro , Bacillus CLPs revealed an inhibitory effect on M. canis growth which is a zoonotic pathogen that causes ringworms. This study suggests an alternative approach to control the growth of M. canis using substances obtained from B. subtilis as a biomedicine agent with antifungal activity.

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Microtiter method to monitor Corynespora cassiicola and sensitivity of the pathogen to carbendazim, prothioconazole and pyraclostrobin

Cited by 10 publications

References 17 publications

WITHDRAWN: Global metabolic changes by Bacillus cyclic lipopeptide extracts on stress responses of para rubber leaf

WITHDRAWN: Global metabolic changes by Bacillus cyclic lipopeptide extracts on stress responses of para rubber leaf

Resistance ofCorynespora cassiicolafrom soybean to QoI and MBC fungicides in Brazil

Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats

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