Microstructure of a Biocatalytic Latex Coating Containing Viable Escherichia coli Cells

Method for Construction of a Simple Laboratory‐Scale Nonwoven Filament Biocatalytic Filter

“…The presence of the top coat decreases the amount of AZC released by 3-4-fold, resulting in <10% leaching of the entrapped AZC. The permeability of the top coat is greater for TC + gly as indicated by the more rapid AZC release compared to TCgly (6,7). This confims our previous observation of glycerol acting as a noncompatible plasticizer reducing the coalescence of the latex particles, which results in a polymeric film of greater permeability compared to when glycerol is absent (6,7).…”

“…The permeability of the top coat is greater for TC + gly as indicated by the more rapid AZC release compared to TCgly (6,7). This confims our previous observation of glycerol acting as a noncompatible plasticizer reducing the coalescence of the latex particles, which results in a polymeric film of greater permeability compared to when glycerol is absent (6,7). The behavior of the AZC release kinetics is similar to that observed in earlier studies performed at 30°C in a scintillation vial containing 20 mL of PBS and 4 m of coated thread in the form of a loose coil (4).…”

Microstructure Evolution in Polymer Latex Coatings for Whole-Cell Biocatalyst Application

“…Recent research with high cell densities of metabolically active but nongrowing Escherichia coli in coatings of a nontoxic latex of vinyl acetate/acrylic acid copolymer demonstrates that these issues can be dealt with successfully (1)(2)(3). A pastelike suspension of about equal volume fractions of cells and latex particles can be coated as a layer 30 -60 m thick on a polyester sheet or other flat substratum.…”

Ink-Jet Printing of Gluconobacter oxydans: Micropatterned Coatings As High Surface-to-Volume Ratio Bio-Reactive Coatings

“…Images of the top surface of rehydrated latex coatings of both E. coli and G. oxydans show that coatings maintain their polymer backbone after rehydration, but oblong holes appear in the polymer matrix at places from where the bacterial cells have left during rehydration [22,29]. The dimensions of G. oxydans in suspension and entrapped in latex coatings is <1 µm by ~2-4 µm, and therefore, the estimated average printed coating thickness over the entire dot is about one or three cells-thick for the two droplets or the three droplets dot microstructures, respectively.…”

“…Their thinness, engineered adhesion, nanoporous microstructure, mass-transfer properties [21][22][23], high-entrapped cell density and ability to be stored partially desiccated at ambient temperature differ significantly from the entrapment of viable microorganisms in crosslinked micro-porous carbohydrate gels, ceramic matrices, monoliths, soft synthetic polymer matrices (such as alginate or polyacrylamide) or behind membranes.…”