Ink-Jet Printing of Gluconobacter oxydans: Micropatterned Coatings As High Surface-to-Volume Ratio Bio-Reactive Coatings

Abstract

Abstract:We formulated a latex ink for ink-jet deposition of viable Gram-negative bacterium Gluconobacter oxydans as a model adhesive, thin, highly bio-reactive microstructured microbial coating. Control of G. oxydans latex-based ink viscosity by dilution with water allowed ink-jet piezoelectric droplet deposition of 30 × 30 arrays of two or three droplets/dot microstructures on a polyester substrate. Profilometry analysis was used to study the resulting dry microstructures. Arrays of individual dots with base diameters of ~233-241 µm were obtained. Ring-shaped dots with dot edges higher than the center, 2.2 and 0.9 µm respectively, were obtained when a one-to-four diluted ink was used. With a less diluted ink (one-to-two diluted), the microstructure became more uniform with an average height of 3.0 µm , but the ink-jet printability was more difficult. Reactivity of the ink-jet deposited microstructures following drying and rehydration was studied in a non-growth medium by oxidation of 50 g/L D-sorbitol to L-sorbose, and a high dot volumetric reaction rate was measured (~435 g· L −1 · h −1 ). These results indicate that latex ink microstructures generated by ink-jet printing may hold considerable potential for 3D fabrication of high surface-to-volume ratio biocoatings for use as microbial biosensors with the aim of coating microbes as reactive biosensors on electronic devices and circuit chips. OPEN ACCESSCoatings 2014, 4 2

Biocatalytic coatings for air pollution control: A proof of concept study on VOC biodegradation

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A Paper‐Based Platform for Long‐Term Deposition of Nanoparticles with Exceptional Redispersibility, Stability, and Functionality

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Bioprocess intensification: A route to efficient and sustainable biocatalytic transformations for the future

“…While there is much development of bioinks and bioprinting, most of the current developments in bioinks are hydrogels or reactive polymers for printing 3D structures as tissue scaffolds and tissue/organ engineering [52][53][54]. Bioprinting methods for process intensification differ significantly because the goal is to print highly reactive very thin (<10 μm thick) flexible highly porous biocatalyst layers and multilayer microstructures using rapid drying low viscosity adhesive latex emulsion ink-jet bioinks for intensifying biocatalysis [55,56]. How biocoatings and biocomposites can intensify bioprocess are summarized in Table 1 and in the conceptual schematic in Fig.…”