Microstructure and characteristic properties of gelatin/chitosan scaffold prepared by the freeze-gelation method

Shamloo, Amir; Kamali, Ali; Fard, Mohammad Reza Bahrani

doi:10.1088/2053-1591/ab43ee

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…These, when sublimated give rise to interconnected pores that are geometrically similar to lyophilized crystals. Result also reported by D. Zhang et al (2014) and Shamloo, Kamali, and Fard (2019) when they produced scaffolds by the freeze-drying method. As seems in Figure 2, on the outside outer surface pores were formed that interconnect through axial multichannels that are formed parallel to the direction of solidification in the upper region and perpendicular to the direction of solidification in the lower region.…”

Section: Resultssupporting

confidence: 56%

Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair

Ponciano

Costa

Barbosa

et al. 2021

RSD

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The bone repair process, among other physiological mechanisms, occurs in a harmonious manner throughout the body. However, the presence of some deleterious factors such as surgery, trauma or pathology, can interfere with the physiology of bone remodeling. It´s Known that the synergistic antimicrobial action between drugs and biomaterials can help and favor osteogenesis after grafting surgery. Therefore, this study aims to develop chitosan / hydroxyapatite scaffolds with amoxicillin for bone repair in the oral cavity. For this, raw materials were selected and characterized and scaffolds were made by the processes of solubilization, dispersion and lyophilization. The characterizations were made by optical microscopy, scanning electron microscopy, eenergy dispersive spectrometer, swelling potential, degradation analysis, apparent porosity test and in vitro cell cytotoxicity. The scaffolds produced in this research showed to have not only adequate physical characteristics but also chemical and biological ones. It was as well as detected a reproducible methodology that resulted in a biomaterial with morphology that provided a high degree of swelling, porosity, satisfactory degradation and the presence of amoxicillin which was confirmed by the eenergy dispersive spectrometer. Nevertheless, the scaffolds with 30% hydroxyapatite showed the best results for in vitro cell viability. So, it is possible to conclude that the scaffolds produced show successful characteristics for bone repair in the oral cavity.

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Section: Resultssupporting

confidence: 56%

Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair

Ponciano

Costa

Barbosa

et al. 2021

RSD

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“…TE is a multidisciplinary scientific field that combines cells, biomaterial scaffolds, and physical/chemical cues with the aim of developing biological substitutes to overcome the significant shortage of tissues and organs for transplantation. Due to the scaffold's importance as structural support for cell growth and differentiation, a broad variety of biomaterials and conventional scaffold fabrication methods have been investigated [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

et al. 2022

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Understanding the mechano–biological coupling mechanisms of biomaterials for tissue engineering is of major importance to assure proper scaffold performance in situ. Therefore, it is of paramount importance to establish correlations between biomaterials, their processing conditions, and their mechanical behaviour, as well as their biological performance. With this work, it was possible to infer a correlation between the addition of graphene nanoparticles (GPN) in a concentration of 0.25, 0.5, and 0.75% (w/w) (GPN0.25, GPN0.5, and GPN0.75, respectively) in three-dimensional poly(ε-caprolactone) (PCL)-based scaffolds, the extrusion-based processing parameters, and the lamellar crystal orientation through small-angle X-ray scattering experiments of extruded samples of PCL and PCL/GPN. Results revealed a significant impact on the scaffold’s mechanical properties to a maximum of 0.5% of GPN content, with a significant improvement in the compressive modulus of 59 MPa to 93 MPa. In vitro cell culture experiments showed the scaffold’s ability to support the adhesion and proliferation of L929 fibroblasts (fold increase of 28, 22, 23, and 13 at day 13 (in relation to day 1) for PCL, GPN0.25, GPN0.5, and GPN0.75, respectively) and bone marrow mesenchymal stem/stromal cells (seven-fold increase for all sample groups at day 21 in relation to day 1). Moreover, the cells maintained high viability, regular morphology, and migration capacity in all the different experimental groups, assuring the potential of PCL/GPN scaffolds for tissue engineering (TE) applications.

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“…Within another trend, the microstructure of cryo-fixed samples is considered as near-real state. This point of view is often supported by correlation with optical or confocal laser scanning microscopy (LSM) images, as was shown, e.g., for foamed fracturing fluids, emulsions formed with apple sourced-pectin, nanoparticle dispersions, mineral clays 8 , 11 – 15 . Noticed differences in microstructure dimension (if compare LSM and cryo-EM results) were not interpreted and, thus, confusing 8 .…”

Section: Introductionmentioning

confidence: 86%

“…The problem is further complicated by obvious similarities between cryo-fixed structures of solutions and dispersions of different chemical nature of solutes (clay minerals, polysaccharides, colloidal gels, etc. ): honeycomb, spongy and lamellae microstructures have been commonly observed 10 , 11 , 14 , 15 . These similarities in microstructure were usually interpreted as a proof for freezing artefacts existence which do not represent reality 8 , 9 .…”

Section: Introductionmentioning

confidence: 99%

“…In comparison with biological objects, non-biological aqueous systems (e.g., solutions, dispersions, gels, clays) do not possess distinguished (dense) phase boundaries in water, and the interpretation of their cryo-EM results is still ambiguous and splitted into two contradictory trends. According to one trend, the microstructure observed at cryo-fixed samples is interpreted as a results of aqueous crystallites formation—as freezing artefact 5 – 11 . The latter appears when dissolved/dispersed substances diffuse towards ice crystal periphery leading to the formation of structure 7 .…”

Section: Introductionmentioning

confidence: 99%

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Cryo-SEM and confocal LSM studies of agar gel, nanoparticle hydrocolloid, mineral clays and saline solutions

Ivashchenko

2022

Sci Rep

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Cryogenic electron microscopy became a powerful tool to study biological objects. For non-biological objects (solutions, gels, dispersions, clays), the polemic about interpretation of cryogenic microscopy results is still in progress splitting on two contradictive trends: considering structure as a near-real state of the sample or as freezing artefacts. In this study, a microstructure of a range of stable aqueous solutions and dispersions (agar, kaolin, montmorillonite, nanoparticles) was investigated by means of cryo-SEM and confocal LSM in order to compare cryo-fixed and unfrozen structures. Noticed correlation between these two methods for studied systems (agar, kaolin, montmorillonite, NPs) allowed to state that ordered microstructure is an inherent feature of these systems. Some inconsistencies in microstructure dimensions were discussed and prescribed to the differences in the bulk and interface layers. Supposedly, NaCl solutions also possess dynamic (femtosecond level) microstructure of neat water clusters and solvated Na+ and Cl- ions that may have an impact on electrolyte abnormal properties.

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Microstructure and characteristic properties of gelatin/chitosan scaffold prepared by the freeze-gelation method

Cited by 8 publications

References 37 publications

Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair

Chitosan and hydroxyapatite scaffolds with amoxicillin for bone repair

Additive Manufactured Poly(ε-caprolactone)-graphene Scaffolds: Lamellar Crystal Orientation, Mechanical Properties and Biological Performance

Cryo-SEM and confocal LSM studies of agar gel, nanoparticle hydrocolloid, mineral clays and saline solutions

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